Data of self-made Taq DNA polymerase prepared for screening purposes.

Data of self-made Taq DNA polymerase prepared for screening purposes.

DNA analysis is a key procedure in genetic engineering. Nowadays, the analysis is often made by PCR with the Taq DNA polymerase. Although the last enzyme price is quite low, the demand for many analyzes leads to many money expenses that are not affordable for many laboratories. In another, many screening tasks do not require highly purified enzyme. Given the unique properties of enzyme that it simplifies its production slightly without resorting to expensive or long techniques such as column chromatography and / or dialysis. Here, the data of the routine use of Taq DNA polymerase prepared according to the protocol developed in our laboratory are presented.

The protocol only takes several hours and does not need qualified personnel or expensive equipment. Yet it gives the preparation of the enzyme adapted to most screening objectives. The isolated Taq DNA polymerase stock can be stored as a suspension of ammonium sulphate in a refrigerator for a long time, at least 6 months. The work enzyme solution is prepared from the on-demand stock suspension, no more than once a month and can also be stored in a refrigerator. The new generation sequencing technology has allowed the detection of rare genetic or somatic mutations and contributed to our understanding of the progression and evolution of the disease.

However, many new generation sequencing technologies first rely on the amplification of DNA, via the reaction of the polymerase chain (PCR), as part of the sample preparation workflow. The errors performed during PCR appear in sequencing data and contribute to false mutations that can define a genetic analysis. In this ratio, a sequencing test at a molecule at a molecule was used to completely catalog the different types of errors introduced during the PCR, including a polymerase interporation, a model switching induced by the structure, a structure. recombination mediated by PCR and DNA damage. In addition to the well-characterized polymerase basic substitution errors, other sources of error have been considered widespread. PCR-mediated recombination per TAQ polymerase has been observed at the single molecule, and surprising would occur as often as basic polymerase substitution errors suggesting that it may be a source of underestimated error for multiplex amplification reactions.

 

A new method based on fast and fast PCR for genotype mice with a mutation of the leptin receptors (DB / DB mouse).

DB / DB mice are one of the most widely used animal models to study cellular and molecular mechanisms of metabolic disorders, such as diabetes, hyperlipidemia and obesity. The mice carry spontaneous punctual mutations in the gene coding the leptin receptor, resulting in the inactivation of the receptors of the leptin. Since homozygous DB / DB mice are sterile, dB / dB mouse maintenance requires reproduction between heterozygous pairs, which makes genotyping essential to the identification of the offspring. The purpose of this study was to develop a fast and very repeatable method for the genotyping of DB / DB mice, which included only three simple steps: genomic DNA is extracted from third tail or ear notches via a alkaline lysis (approximately20 min);

The samples are then subjected to the reaction of the chain of the chain of the refractory mutation of the tetra-primer-amplification amplification (PCR) using specially designed and validated primer assemblies (~ 17 h); Finally, genotypes are determined by solving PCR products on regular DNA electrophoresis (~ 10 min). The entire DB / DB mouse genotyping procedure can be performed using a regular amplification of the Taq polymerase and PCR within 2 h. The other advantages of this method include high sensitivity and reproducibility. Minimum amounts of mouse tissue are required and genomic samples of the DNA can be stably stored at room temperature for a maximum month. In conclusion, the method is simple, cost-effective, sensitive and reliable, which will greatly facilitate studies using DB / DB mice.

 Data of self-made Taq DNA polymerase prepared for screening purposes.
Data of self-made Taq DNA polymerase prepared for screening purposes.

Association of polymorphism of vitamin D receptor genes in patients with type 2 diabetes in the Kashmir Valley.

GOAL
About 1 billion people in various ethnic and elderly groups have vitamin D deficiency. The high prevalence of such a disability is an imperative public health problem, because hypovitaminosis D is an autonomous risk factor for mortality in the mortality. population in general. Beyond bone integrity and calcium homeostasis, it is involved in many physiological and pathological processes. The role of vitamin D in the pathogenesis and prevention of type 2 diabetes mellitus aroused universal interest.

Methods
This case study at the hospital has been designed to study the combination between 25-hydroxy vitamin D (25 [oh] d) levels and polymorphism of vitamin D genes (VDR) with diabetes and to evaluate their roles As factors of risk of diabetes. 100 cases and controls have been taken. 25 (OH) D levels were analyzed by the chemioleneescence method using a Siemens Advia Centaur analyzer. The genomic DNA has been extracted and the Taq-1 and BSM-1 genotyping in the VDR gene has been performed using the reaction of the polymerization chain followed by a restriction fragment length polymorphism (PCR-RFLP) .

RESULTS
(Oh) The levels of patients with diabetes were significantly lower than those of the controls (19.26 ± 0.95 ng / ml against 25.49 ± 1.02 ng / ml; p = 0.001). (OH) The levels of have been found inversely associated with percentages of glyceated hemoglobin in cases (R2 = 0.74). The results suggest that BSM-1 allele and B (G ALLLE) simple nucleotide polymorphisms could be a susceptibility allele for the diabetes of the Cashmiri population.

Measles Virus Large Polymerase (58-149) (Recombinant)

22060553-1 100 µg
EUR 264.47

Recombinant Measles Virus Large Polymerase (58-149)

7-07837 100µg Ask for price

Recombinant Measles Virus Large Polymerase (58-149)

7-07838 500µg Ask for price

Recombinant Measles Virus Large Polymerase (58-149)

7-07839 1000µg Ask for price

OPPA01109-100UG - MV Large Polymerase 2059-2183 a.a Protein

OPPA01109-100UG 100ug
EUR 278

Recombinant (E.Coli) Measles Virus Large Polymerase (58-149)

RP-651 100 ug
EUR 343.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-1mg

QP12758-1mg 1mg
EUR 1513.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-100ug

QP12758-100ug 100ug
EUR 261.6

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-500ug

QP12758-500ug 500ug
EUR 795.6

Recombinant (E.Coli) purified Measles virus Large Polymerase (58-149)

RP-1613 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 58-149)

VAng-Lsx0397-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 58-149), recombinant protein from E. coli.

Recombinant MeV Large Polymerase Protein

VAng-Lsx0385-inquire inquire Ask for price
Description: Recombinant MeV Large Polymerase containing the large polymerase immunodominant regions, 58-149 amino acids was expressed in E. coli and purified by proprietary chromatographic technique.

Bst DNA Polymerase, Large Fragment

M1213-200 each
EUR 346.8

Bsu DNA Polymerase Large Fragment

M1214-200 each
EUR 477.6

Bst DNA Polymerase Large Fragment

P701-01 800 U
EUR 146.4

Bst DNA Polymerase Large Fragment

P701-02 8000 U
EUR 325.2

Bsu DNA Polymerase Large Fragment

3587 2000Units(5units/µl)
EUR 316

Measles Virus Large Polymerase (2059-2183) Protein

20-abx260297
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  • 0.5 mg
  • 100 ug
  • 1 mg

Measles Virus Large Polymerase (2059-2183) (Recombinant)

22060555-1 100 µg
EUR 264.47

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07843 100µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07844 500µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07845 1000µg Ask for price

Bst II DNA Polymerase Large Fragment

P702-01 1,600 U
EUR 27.5

DNA Polymerase I Large (Klenow) Fragment

M1212-100 each
EUR 333.6

DNA Polymerase I, Large (Klenow) Fragment

RK20525 200U
EUR 29

Recombinant (E.Coli) Measles Virus Large Polymerase (2059-2183)

RP-653 100 ug
EUR 343.2

Bst II Pro DNA Polymerase Large Fragment

P703-01 1,600 U
EUR 36.73

Bst II Pro DNA Polymerase Large Fragment

P703-02 8,000 U
EUR 145

Bsu DNA Polymerase Large Fragment - 200 units

3582 200units
EUR 65
Description: Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity (1)Product Includes:Bsu DNA Polymerase I, Large fragment10x Bsu DNA Polymerase I reaction buffer

Sau DNA Polymerase Large Fragment - 200 units

3592 200units
EUR 115
Description: Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.Product Includes:Sau DNA Polymerase I, Large fragment10x Sau DNA Polymerase I reaction buffer

Glycerol Free Bsu DNA Polymerase Large Fragment

3582GF 200Units(5units/µl)
EUR 124

Glycerol Free Bsu DNA Polymerase Large Fragment

3585GF 1000Units(5units/µl
EUR 360

Bst II LAMP DNA Polymerase (Large Fragment) 800U

DP003-0100 100 μl
EUR 15

Recombinant (E.Coli) purified Measles virus Large Polymerase (2059-2183)

RP-1612 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 2059-2183)

VAng-Lsx0399-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 2059-2183), recombinant protein from E. coli.

DNA Polymerase I, Large (Klenow) Fragment - 1,000 units

3262 1/EA
EUR 346.8

Recombinant MeV Polymerase Protein (aa 58-149)

VAng-Lsx0394-inquire inquire Ask for price
Description: MeV Polymerase (aa 58-149), recombinant protein from E. coli.

Cenarchaeum symbiosum DNA polymerase II large subunit (polC)

1-CSB-EP370095DRA
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial expressed in E.coli

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC), partial

CSB-EP370095DRA 3498 mg Ask for price

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial

AP77127 1mg
EUR 3015

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-100ug 100ug
EUR 735.9

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-1mg 1mg
EUR 3078.4

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-20ug 20ug
EUR 519.2

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

Taq Polymerase

PCR-211L 5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-211S 200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-420-10KU 10kilo units
EUR 1043.5

Taq Polymerase

PCR-420-1KU 1kilo unit
EUR 222.3

Taq Polymerase

MBT060-1000U 1 unit
EUR 52.73
Description: Taq Polymerase

Taq Polymerase

MBT060-5000U 1 unit
EUR 204.76
Description: Taq Polymerase

Taq Polymerase

MBT060-500U 1 unit
EUR 35.36
Description: Taq Polymerase

DNA Polymerase

abx461001-1096tests 10 × 96 tests Ask for price

DNA Polymerase

abx461001-596tests 5 × 96 tests Ask for price

DNA Polymerase

abx461001-96tests 96 tests
EUR 425

mtRNA polymerase

E8ER1913-34 100ul
EUR 275
Description: Available in various conjugation types.

HBV polymerase (455 - 463)

5-01273 4 x 5mg Ask for price

(untagged)-Human cDNA FLJ30579 fis, clone BRAWH2006989, weakly similar to DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT (EC 2.7.7.6)

SC312687 10 µg Ask for price

T7 RNA Polymerase (5000U)

9K-005-0004 5000U
EUR 316.82

T7 RNA Polymerase

E041 100 μl (5000 U)
EUR 65
Description: T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences.

DNA polymerase mu

E8ER1907-58 100ul
EUR 275
Description: Available in various conjugation types.

T4 DNA Polymerase

M1211-100 each
EUR 385.2

T7 RNA Polymerase

K1083-100000 100000U
EUR 512
Description: DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences

T7 RNA Polymerase

K1083-25000 25000U
EUR 160
Description: DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences

T7 RNA Polymerase

K1083-5000 5000U
EUR 40
Description: DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences

T7 RNA Polymerase

PCR-603L 5 x 4000 units
EUR 251.6

T7 RNA Polymerase

PCR-603S 4000units
EUR 63

T4 DNA polymerase

N101-01 2000 U
EUR 223

T7 RNA Polymerase

3292 5,000Units
EUR 57.6

T7 RNA Polymerase

3295 25,000Units
EUR 220

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

SP6 RNA Polymerase (5000U)

9K-005-0003 5000U
EUR 462.47

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Pfu DNA Polymerase

EQ008 50μl
EUR 68

Pfu DNA Polymerase

enz-265 100U
EUR 145
Description: Recombinant Pfu DNA Polymerase

Taq DNA Polymerase

G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

PFU DNA Polymerase

9003-2500 each
EUR 614.4

PFU DNA Polymerase

9003-500 each
EUR 216

Laq? DNA Polymerase

9004-2500 each
EUR 732

Laq? DNA Polymerase

9004-500 each
EUR 261.6

Taq DNA Polymerase

L7051001 1000 unit
EUR 167

Taq DNA Polymerase

L7051200 200 unit
EUR 43

SP6 RNA Polymerase

K1096-10000 10000U
EUR 72
Description: SP6 RNA Polymerase, highly specific identification of SP6 promoter sequences

SP6 RNA Polymerase

K1096-2000 2000U
EUR 28
Description: SP6 RNA Polymerase, highly specific identification of SP6 promoter sequences

SP6 RNA Polymerase

K1096-5000 5000U
EUR 40
Description: SP6 RNA Polymerase, highly specific identification of SP6 promoter sequences

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

Pfu-X Polymerase

PCR-207L 500units
EUR 362
Description: Pyrococcus furiosus, recombinant, E. coli

Pfu-X Polymerase

PCR-207S 100units
EUR 90.4
Description: Pyrococcus furiosus, recombinant, E. coli

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

DNA Polymerase Mix

abx461002-1096tests 10 × 96 tests Ask for price

DNA Polymerase Mix

abx461002-596tests 5 × 96 tests Ask for price

DNA Polymerase Mix

abx461002-96tests 96 tests
EUR 1262.5

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

DNA Polymerase beta

E8ET1705-12 100ul
EUR 275
Description: Available in various conjugation types.

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Phi29 DNA Polymerase

E014 1000 U (100 μl)
EUR 145
Description: Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.

DNA polymerase delta

E8ER1907-56 100ul
EUR 275
Description: Available in various conjugation types.

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 104.4

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 189.6

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

Ready? DNA Polymerase

M1146-1000 each
EUR 398.4

Ready? DNA Polymerase

M1146-10000 each
EUR 1279.2

Ready? DNA Polymerase

M1146-5000 each
EUR 960

Phi29 DNA Polymerase

M1239-1000 each
EUR 373.2

phi29 DNA Polymerase

RK21002 1250U
EUR 4

Phi29 DNA Polymerase

PCR-381L 5 x 200units
EUR 255
Description: Bacillus subtilis phage phi29, recombinant, E. coli

Phi29 DNA Polymerase

PCR-381S 200units
EUR 63.8
Description: Bacillus subtilis phage phi29, recombinant, E. coli

Ultra DNA Polymerase

PCR-391L 5 x 100units
EUR 486.7
Description: also known as Phusion High-Fidelity DNA Polymerase

Ultra DNA Polymerase

PCR-391S 100units
EUR 121.7
Description: also known as Phusion High-Fidelity DNA Polymerase

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

Breeze? DNA Polymerase

M1148-1000 each
EUR 1246.8

Breeze? DNA Polymerase

M1148-250 each
EUR 464.4

Conclusions
The gene polymorphisms of the VDR appear to be an important genetic determinant in the progression of diabetes. Considering the important predisposition risk factor, we observed that Taq-1 and BSM-1 were strongly associated with Northern Indian diabetes. But requires a subsequent study as a probable genetic risk marker for diabetes.

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