Data of self-made Taq DNA polymerase prepared for screening purposes.

Data of self-made Taq DNA polymerase prepared for screening purposes.

DNA analysis is a key procedure in genetic engineering. Nowadays, the analysis is often made by PCR with the Taq DNA polymerase. Although the last enzyme price is quite low, the demand for many analyzes leads to many money expenses that are not affordable for many laboratories. In another, many screening tasks do not require highly purified enzyme. Given the unique properties of enzyme that it simplifies its production slightly without resorting to expensive or long techniques such as column chromatography and / or dialysis. Here, the data of the routine use of Taq DNA polymerase prepared according to the protocol developed in our laboratory are presented.

The protocol only takes several hours and does not need qualified personnel or expensive equipment. Yet it gives the preparation of the enzyme adapted to most screening objectives. The isolated Taq DNA polymerase stock can be stored as a suspension of ammonium sulphate in a refrigerator for a long time, at least 6 months. The work enzyme solution is prepared from the on-demand stock suspension, no more than once a month and can also be stored in a refrigerator. The new generation sequencing technology has allowed the detection of rare genetic or somatic mutations and contributed to our understanding of the progression and evolution of the disease.

However, many new generation sequencing technologies first rely on the amplification of DNA, via the reaction of the polymerase chain (PCR), as part of the sample preparation workflow. The errors performed during PCR appear in sequencing data and contribute to false mutations that can define a genetic analysis. In this ratio, a sequencing test at a molecule at a molecule was used to completely catalog the different types of errors introduced during the PCR, including a polymerase interporation, a model switching induced by the structure, a structure. recombination mediated by PCR and DNA damage. In addition to the well-characterized polymerase basic substitution errors, other sources of error have been considered widespread. PCR-mediated recombination per TAQ polymerase has been observed at the single molecule, and surprising would occur as often as basic polymerase substitution errors suggesting that it may be a source of underestimated error for multiplex amplification reactions.

 

A new method based on fast and fast PCR for genotype mice with a mutation of the leptin receptors (DB / DB mouse).

DB / DB mice are one of the most widely used animal models to study cellular and molecular mechanisms of metabolic disorders, such as diabetes, hyperlipidemia and obesity. The mice carry spontaneous punctual mutations in the gene coding the leptin receptor, resulting in the inactivation of the receptors of the leptin. Since homozygous DB / DB mice are sterile, dB / dB mouse maintenance requires reproduction between heterozygous pairs, which makes genotyping essential to the identification of the offspring. The purpose of this study was to develop a fast and very repeatable method for the genotyping of DB / DB mice, which included only three simple steps: genomic DNA is extracted from third tail or ear notches via a alkaline lysis (approximately20 min);

The samples are then subjected to the reaction of the chain of the chain of the refractory mutation of the tetra-primer-amplification amplification (PCR) using specially designed and validated primer assemblies (~ 17 h); Finally, genotypes are determined by solving PCR products on regular DNA electrophoresis (~ 10 min). The entire DB / DB mouse genotyping procedure can be performed using a regular amplification of the Taq polymerase and PCR within 2 h. The other advantages of this method include high sensitivity and reproducibility. Minimum amounts of mouse tissue are required and genomic samples of the DNA can be stably stored at room temperature for a maximum month. In conclusion, the method is simple, cost-effective, sensitive and reliable, which will greatly facilitate studies using DB / DB mice.

 Data of self-made Taq DNA polymerase prepared for screening purposes.
Data of self-made Taq DNA polymerase prepared for screening purposes.

Association of polymorphism of vitamin D receptor genes in patients with type 2 diabetes in the Kashmir Valley.

GOAL
About 1 billion people in various ethnic and elderly groups have vitamin D deficiency. The high prevalence of such a disability is an imperative public health problem, because hypovitaminosis D is an autonomous risk factor for mortality in the mortality. population in general. Beyond bone integrity and calcium homeostasis, it is involved in many physiological and pathological processes. The role of vitamin D in the pathogenesis and prevention of type 2 diabetes mellitus aroused universal interest.

Methods
This case study at the hospital has been designed to study the combination between 25-hydroxy vitamin D (25 [oh] d) levels and polymorphism of vitamin D genes (VDR) with diabetes and to evaluate their roles As factors of risk of diabetes. 100 cases and controls have been taken. 25 (OH) D levels were analyzed by the chemioleneescence method using a Siemens Advia Centaur analyzer. The genomic DNA has been extracted and the Taq-1 and BSM-1 genotyping in the VDR gene has been performed using the reaction of the polymerization chain followed by a restriction fragment length polymorphism (PCR-RFLP) .

RESULTS
(Oh) The levels of patients with diabetes were significantly lower than those of the controls (19.26 ± 0.95 ng / ml against 25.49 ± 1.02 ng / ml; p = 0.001). (OH) The levels of have been found inversely associated with percentages of glyceated hemoglobin in cases (R2 = 0.74). The results suggest that BSM-1 allele and B (G ALLLE) simple nucleotide polymorphisms could be a susceptibility allele for the diabetes of the Cashmiri population.

Recombinant Measles Virus Large Polymerase (58-149)

7-07838 500µg Ask for price

Recombinant Measles Virus Large Polymerase (58-149)

7-07839 1000µg Ask for price

Recombinant (E.Coli) Measles Virus Large Polymerase (58-149)

RP-651 100 ug
EUR 343.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-1mg

QP12758-1mg 1mg
EUR 1513.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-100ug

QP12758-100ug 100ug
EUR 261.6

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-500ug

QP12758-500ug 500ug
EUR 795.6

Recombinant (E.Coli) purified Measles virus Large Polymerase (58-149)

RP-1613 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 58-149)

VAng-Lsx0397-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 58-149), recombinant protein from E. coli.

Recombinant MeV Large Polymerase Protein

VAng-Lsx0385-inquire inquire Ask for price
Description: Recombinant MeV Large Polymerase containing the large polymerase immunodominant regions, 58-149 amino acids was expressed in E. coli and purified by proprietary chromatographic technique.

Bst DNA Polymerase Large Fragment

P701-01 800 U
EUR 146.4

Bst DNA Polymerase Large Fragment

P701-02 8000 U
EUR 325.2

Bst DNA Polymerase, Large Fragment

M1213-200 each
EUR 346.8

Bsu DNA Polymerase Large Fragment

M1214-200 each
EUR 477.6

Measles Virus Large Polymerase (2059-2183) Protein

20-abx260297
  • EUR 1062.00
  • EUR 410.40
  • EUR 1930.80
  • 0.5 mg
  • 100 ug
  • 1 mg

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07843 100µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07844 500µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07845 1000µg Ask for price

Bst II DNA Polymerase Large Fragment

P702-01 1,600 U
EUR 22

DNA Polymerase I Large (Klenow) Fragment

M1212-100 each
EUR 333.6

Recombinant (E.Coli) Measles Virus Large Polymerase (2059-2183)

RP-653 100 ug
EUR 343.2

Bst II Pro DNA Polymerase Large Fragment

P703-01 1,600 U
EUR 29.39

Bst II Pro DNA Polymerase Large Fragment

P703-02 8,000 U
EUR 116

Recombinant (E.Coli) purified Measles virus Large Polymerase (2059-2183)

RP-1612 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 2059-2183)

VAng-Lsx0399-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 2059-2183), recombinant protein from E. coli.

DNA Polymerase I, Large (Klenow) Fragment - 1,000 units

3262 1/EA
EUR 346.8

Recombinant MeV Polymerase Protein (aa 58-149)

VAng-Lsx0394-inquire inquire Ask for price
Description: MeV Polymerase (aa 58-149), recombinant protein from E. coli.

Cenarchaeum symbiosum DNA polymerase II large subunit (polC)

1-CSB-EP370095DRA
  • EUR 745.20
  • EUR 457.20
  • EUR 2331.60
  • EUR 1058.40
  • EUR 1602.00
  • EUR 541.20
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial expressed in E.coli

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

HBV polymerase (455 - 463)

5-01273 4 x 5mg Ask for price

T7 RNA Polymerase (5000U)

9K-005-0004 5000U
EUR 316.82

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

T4 DNA Polymerase

M1211-100 each
EUR 385.2

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

SP6 RNA Polymerase (5000U)

9K-005-0003 5000U
EUR 462.47

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

PFU DNA Polymerase

9003-2500 each
EUR 614.4

PFU DNA Polymerase

9003-500 each
EUR 216

Laq? DNA Polymerase

9004-2500 each
EUR 732

Laq? DNA Polymerase

9004-500 each
EUR 261.6

Taq DNA Polymerase

L7051001 1000 unit
EUR 159

Taq DNA Polymerase

L7051200 200 unit
EUR 41

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

Ready? DNA Polymerase

M1146-1000 each
EUR 398.4

Ready? DNA Polymerase

M1146-10000 each
EUR 1279.2

Ready? DNA Polymerase

M1146-5000 each
EUR 960

Phi29 DNA Polymerase

M1239-1000 each
EUR 373.2

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 104.4

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 189.6

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

AceTaq DNA Polymerase

P401-d1 250 U
EUR 163.2

AceTaq DNA Polymerase

P401-d2 1000 U
EUR 272.4

AceTaq DNA Polymerase

P401-d3 3000 U
EUR 536.4

Breeze? DNA Polymerase

M1148-1000 each
EUR 1246.8

Breeze? DNA Polymerase

M1148-250 each
EUR 464.4

AceTaq DNA Polymerase

P401-MD1 250 U
EUR 46.4

Distant? DNA Polymerase

M1150-1000 each
EUR 823.2

Distant? DNA Polymerase

M1150-250 each
EUR 333.6

Advance? DNA Polymerase

M1151-1000 each
EUR 895.2

Advance? DNA Polymerase

M1151-250 each
EUR 373.2

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

anti-mtRNA polymerase

YF-PA24420 50 ul
EUR 400.8
Description: Mouse polyclonal to mtRNA polymerase

anti-mtRNA polymerase

YF-PA13878 50 ug
EUR 435.6
Description: Mouse polyclonal to mtRNA polymerase

E. coli RNA polymerase Assay Kit Plus (E. coli RNA polymerase included)

RPA100KE 100 assays
EUR 467.15
Description: This product includes all the reagents in E. coli RNA Polymerase Assay Kit (Catalog number RPA100K) plus the enzyme, 33 ul of 100 x E. coli RNAP. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.

Taq HS DNA Polymerase

P132-d1 500 U
EUR 38.28

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 71.34

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 324.8

DNA polymerase ? (C-His)

10-105 50ug
EUR 637.2
Description: The DNA polymerase ? (C-His) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 104.4

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

T4 DNA Polymerase - 500 units

3222 1/EA
EUR 247.2

HBV polymerase (455 463) Peptide

20-abx266100
  • EUR 493.20
  • EUR 794.40
  • EUR 376.80
  • 10 mg
  • 25 mg
  • 5 mg

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Poly(A) Polymerase Yeast

M1215-100 each
EUR 333.6

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 620.4

MyTaq HS DNA Polymerase

BIO-21111 250 units Ask for price

MyTaq HS DNA Polymerase

BIO-21111/S Sample Ask for price

MyTaq HS DNA Polymerase

BIO-21112 1000 units Ask for price

MyTaq HS DNA Polymerase

BIO-21113 2500 units Ask for price

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

ACTaq? Long DNA Polymerase, 1000U

E2200-1000U each
EUR 282

T4 DNA Polymerase (5,000 U/ml)

RK20539 25 U Ask for price

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (+dNTPs)

02-021 200U
EUR 214.8
Description: The Pfu DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (+dNTPs)

02-021-5 5×200U
EUR 402
Description: The Pfu DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (-dNTPs)

02-031 200U
EUR 199.2
Description: The Pfu DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (-dNTPs)

02-031-5 5×200U
EUR 339.6
Description: The Pfu DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

T DNA Polymerase Enzyme

abx071008-250U 250 U
EUR 226.8

T DNA Polymerase Enzyme

abx071008-3kU 3 kU
EUR 543.6

T DNA Polymerase Enzyme

abx071008-500U 500 U
EUR 260.4

MyTaq Red DNA Polymerase

BIO-21108 500 units Ask for price

MyTaq Red DNA Polymerase

BIO-21108/S Sample Ask for price

MyTaq Red DNA Polymerase

BIO-21109 2500 units Ask for price

MyTaq Red DNA Polymerase

BIO-21110 5000 units Ask for price

HBVpol502, HBV polymerase (502 - 510)

5-01274 4 x 5mg Ask for price

HBVpol655, HBV polymerase (655 - 663)

5-01275 4 x 5mg Ask for price

REV1 Polymerase (Recombinant)

20-abx073440
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Outstretched? DNA Polymerase

M1152-1000 each
EUR 960

Outstretched? DNA Polymerase

M1152-250 each
EUR 398.4

T4 DNA Polymerase, 5u/ul

BEP0061 100U
EUR 153.97

Conclusions
The gene polymorphisms of the VDR appear to be an important genetic determinant in the progression of diabetes. Considering the important predisposition risk factor, we observed that Taq-1 and BSM-1 were strongly associated with Northern Indian diabetes. But requires a subsequent study as a probable genetic risk marker for diabetes.

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