Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Trial chain tests of the three duplex transcription of the three duplex transcription (real-time RT-PCR) transcription based on Taqman chemistry, have been developed for simultaneous detection and the specific quantization of the virus Apple chlorotic leaves (ACLSV), plum pox virus (PPV), Prunus NecRotic Ringspot Virus (PNRSV), DNARE Virus (PDV), Latent Mosaic Virus (PLMVD) (PLMVD) and Fruit Phytoplasma European stone (Esfy), which are considered among the most important pathogens affecting stone fruit trees. The RT-PCR (RT-QPCR) quantitative analyzes have been optimized using RNA transcripts (plasmid linearized has been used for optimizing the determination of the esfy phytoplasm) of known concentrations. No difference in sensitivity was recorded between the duplex and RT-QPCR analyzes of RT-QPCR. The amplification efficiency of the recto-95.8% tests is 91.1 to 95.8%, while the linear quantization range was between 20 and 2 x 107 transcripts of linearized plasmid for PLMVD and phytoplasma esfy, 40 to 4 x 107 RNA transcripts for ACLSV, PPV and PDV and 102 to 108 RNA transcripts for PUNSV, respectively

Duplex RT-QPCR assays, which have been validated using all the two isolates characterized by all pathogens and field samples of Prunus species in northern Greece, have presented a wide range of detection . Overall, developed methods include useful tools that can be applied to simultaneous and reliable detection of transmissible transmissible pathogens in Prunus spp certification programs. Diarrheal diseases account for more than 50% of foodborne diseases around the world, the majority of which occur in infants and young children. The traditional bacterial detection method is complex and takes a long time; Therefore, it is necessary to establish a fast and practical detection method that can detect multiple pathogens simultaneously.

In this study, we have developed a set of five PCR dosages SYBR GREEN I Time-time multiplex to simultaneously detect 15 common pathical agents based on the non-dimeric system assisted by homo-tag. These analyzes efficiently reduce the formation of dimeric primer and improve the stability, uniformity and efficiency of the amplification of multiplex PCR.

The role of polymorphisms Genes of the vitamin D receptor in the risk of colorectal cancer

Vitamin D deficiency has been associated with an increase in the risk of incidence and mortality of colorectal cancer (CRC). The median vitamin D its action through the binding of the vitamin D receiver (VDR) and the polymorphisms of the VDR could explain these reverse associations. The objective of the study was the Survey of the relevance of RS731236; Thermus Aquaticus I (Taqi), ; Acetobacter Pasteurianus Sub. Pasteurianus I, Bacillus Stearothermophilus I (BSMI) Polymorphisms of the VDR gene with colorectal carcinogenesis (CRC) and progression. The peripheral blood was obtained from 397 patients with staging II / III (n = 202) and step IV (n = 195) and IV (n = 195). In addition, samples of 100 healthy donors and 40 patients with adenomatic polyps were also included as control groups.

 

 

Genotyping in patient and control samples was carried out using polymorphisms of fragmentation length of the reaction of the polymerase reaction (PCR-RFLP). An important association has been revealed between the four polymorphisms and cancer. Individuals with homozygous mutant genotypes (TT, AA, FF or BB) were more sensitive to the disease (p <0.001). All detected mutant genotypes have also been significantly associated with Phase IV (p <0.001), resulting in a significant decrease in survival (p <0.001). In addition, the four polymorphisms have been significantly associated with Krasten mutations (Kirsten Ras oncogene) and genetic variants of the toll receptor (TLR2, TLR4 and TLR9).

 Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees
Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Evaluation of seven African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples.

 

The African porcelain fever virus (ASFV) is a complex dual-stranded DNA virus, responsible for a highly infectious and mortal disease in pigs and shores and for a significant deterioration in animal welfare. Over the past decade, the disease has expanded to several European and Asian countries, causing unprecedented dramatic economic losses in the pork industry. In the absence of vaccine, affected countries are based on confidence diagnostic tests and test policies adapted to setting up control programs to combat disease.

In this study, we evaluated the sensitivity and specificity of seven real-time real-time PCR detection kits in the trade and three Taq polymerases on 300 samples of well-characterized boars collected in Belgium during the 2018 epidemic -2019. This study confirms that all commercial kits and two TAQ polymerases are suitable for ASFV detection in diagnostic laboratories. In addition, the use of endogenous controls is underlined when using field samples harvested on carcasses in an advanced decomposition step, in order to avoid falsely negative results. The functional efficacy of the expression cassettes integrated in a plasmid and a PCR-amplified fragment was relatively analyzed after the transitional transition transition in vitro or introduction into the development embryo of Danio Rerio.

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx331383-100ul 100 ul
EUR 510

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

20-abx330112
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx236606-100ug 100 ug
EUR 577.2

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (HRP)

20-abx335409
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx114590
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx327785
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx334340
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx004069
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (FITC)

20-abx335410
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (HRP)

20-abx335400
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Epsilon Catalytic Subunit A (POLE1) Antibody

20-abx014425
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (FITC)

20-abx335401
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (Biotin)

20-abx335411
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (Biotin)

20-abx335402
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (HRP)

20-abx335391
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (FITC)

20-abx335392
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (Biotin)

20-abx335393
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Epstein-Barr virus DNA polymerase catalytic subunit (BALF5)

1-CSB-EP355978EFA
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Epstein-Barr virus DNA polymerase catalytic subunit (BALF5) ,partial expressed in E.coli

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

20-abx325215
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx331357-100ul 100 ul
EUR 510

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx011465-100ug 100 ug
EUR 526.8

Human DNA polymerase zeta catalytic subunit, REV3L ELISA KIT

ELI-47651h 96 Tests
EUR 988.8

Mouse DNA polymerase zeta catalytic subunit, Rev3l ELISA KIT

ELI-09399m 96 Tests
EUR 1038

Mouse DNA polymerase alpha catalytic subunit, Pola1 ELISA KIT

ELI-47649m 96 Tests
EUR 1038

Human DNA polymerase delta catalytic subunit, POLD1 ELISA KIT

ELI-08845h 96 Tests
EUR 988.8

Mouse DNA polymerase delta catalytic subunit, Pold1 ELISA KIT

ELI-08846m 96 Tests
EUR 1038

Human DNA polymerase alpha catalytic subunit, POLA1 ELISA KIT

ELI-32247h 96 Tests
EUR 988.8

Bovine DNA polymerase delta catalytic subunit, POLD1 ELISA KIT

ELI-47805b 96 Tests
EUR 1113.6

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human DNA Polymerase Delta 1, Catalytic Subunit (POLD1) ELISA Kit

abx382340-96tests 96 tests
EUR 1093.2

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Mouse DNA polymerase epsilon catalytic subunit A, Pole ELISA KIT

ELI-08849m 96 Tests
EUR 1038

Human DNA polymerase epsilon catalytic subunit A, POLE ELISA KIT

ELI-27683h 96 Tests
EUR 988.8

Rabbit Anti-Human DNA Polymerase Delta, catalytic subunit (Pol D) antiserum

DNPD11-S 100 ul
EUR 548.4

Human DNA Polymerase Delta, catalytic subunit (Pol D) Control/blocking peptide

DNPD11-P 100 ug
EUR 196.8

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-10ug

QP7276-ec-10ug 10ug
EUR 391.2

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-1mg

QP7276-ec-1mg 1mg
EUR 2763.6

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-50ug

QP7276-ec-50ug 50ug
EUR 522

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-100ug

QP7276-ec-100ug 100ug
EUR 848.4

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-200ug

QP7276-ec-200ug 200ug
EUR 1338

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-500ug

QP7276-ec-500ug 500ug
EUR 1816.8

Recombinant Trypanosoma Brucei DNA polymerase alpha catalytic subunit Protein (aa 1-1339)

VAng-Wyb8056-inquire inquire Ask for price
Description: Trypanosoma Brucei brucei DNA polymerase alpha catalytic subunit protein, partial, recombinant protein.

Recombinant Leishmania Donovani DNA polymerase alpha catalytic subunit Protein (aa 1-1339)

VAng-Wyb7419-inquire inquire Ask for price
Description: Leishmania Donovani DNA polymerase alpha catalytic subunit, partial, recombinant protein.

DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PK) Antibody

20-abx013061
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PKcs) Antibody

abx232474-100ug 100 ug
EUR 610.8

DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PKcs) Antibody

20-abx008825
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase Delta Subunit 4 (POLD4) Antibody

20-abx217846
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA Polymerase Delta Subunit 4 (POLD4) Antibody

20-abx005858
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

POLA2 Antibody / DNA polymerase alpha subunit B

RQ6788 100ug
EUR 356.15
Description: DNA polymerase alpha subunit 2/B is an enzyme that in humans is encoded by the POLA2 gene. POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).

Polymerase (Dna Directed), alpha 2 (70Kd Subunit) Antibody

20-abx114588
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Polymerase (Dna Directed), epsilon 3 (P17 Subunit) Antibody

20-abx114592
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx236607-100ug 100 ug
EUR 577.2

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

abx236608-100ug 100 ug
EUR 610.8

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

20-abx327451
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

20-abx114591
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx034852-400ul 400 ul
EUR 627.6

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx034852-80l 80 µl
EUR 343.2

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx036036-100ug 100 ug
EUR 469.2

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

abx028767-400ul 400 ul
EUR 627.6

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

abx028767-80l 80 µl
EUR 343.2

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

20-abx334271
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

20-abx338731
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

20-abx005465
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

20-abx006001
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Epsilon 4, Accessory Subunit (POLE4) Antibody

20-abx135826
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

abx236610-100ug 100 ug
EUR 577.2

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

20-abx217847
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA Polymerase Epsilon 4, Accessory Subunit (POLE4) Antibody

20-abx325247
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

abx034739-400ul 400 ul
EUR 627.6

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

abx034739-80l 80 µl
EUR 343.2

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

abx033322-400ul 400 ul
EUR 627.6

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

abx033322-80l 80 µl
EUR 343.2

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

20-abx323922
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

20-abx004960
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody (HRP)

20-abx335394
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody (HRP)

20-abx335397
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody (FITC)

20-abx335395
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody (FITC)

20-abx335398
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein Phosphatase 5, Catalytic Subunit Antibody

20-abx114857
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Calpain-1 Catalytic Subunit (CAPN1) Antibody

20-abx321431
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

Calpain-2 Catalytic Subunit (CAPN2) Antibody

20-abx125015
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul

Calpain-1 Catalytic Subunit (CAPN1) Antibody

20-abx320148
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

Calpain-2 Catalytic Subunit (CAPN2) Antibody

20-abx319449
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Calpain-1 Catalytic Subunit (CAPN1) Antibody

abx340186-100ug 100 ug
EUR 469.2

Calpain-1 Catalytic Subunit (CAPN1) Antibody

abx340242-100ug 100 ug
EUR 469.2

Calpain-1 Catalytic Subunit (CAPN1) Antibody

abx432444-200ul 200 ul
EUR 460.8

Calpain-1 Catalytic Subunit (CAPN1) Antibody

abx432445-200ul 200 ul
EUR 460.8

Calpain-2 Catalytic Subunit (CAPN2) Antibody

20-abx005965
  • EUR 493.20
  • EUR 710.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 50 ul

Calpain-2 Catalytic Subunit (CAPN2) Antibody

20-abx006701
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Calpain-1 Catalytic Subunit (CAPN1) Antibody

20-abx001086
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Calpain-1 Catalytic Subunit (CAPN1) Antibody

20-abx302543
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Calpain-1 Catalytic Subunit (CAPN1) Antibody

20-abx302697
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody (Biotin)

20-abx335396
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody (Biotin)

20-abx335399
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA-directed RNA polymerase III subunit RPC3 Antibody (HRP)

20-abx108190
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA-Directed RNA Polymerase III Subunit RPC1 (RPC1) Antibody

20-abx249450
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA-Directed RNA Polymerase III Subunit RPC6 (RPC6) Antibody

20-abx217860
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA-Directed RNA Polymerase III Subunit RPC7 (RPC7) Antibody

20-abx217861
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA-directed RNA polymerase III subunit RPC3 Antibody (FITC)

20-abx106771
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA-Directed RNA Polymerase III Subunit RPC3 (RPC3) Antibody

abx149879-100ug 100 ug
EUR 526.8

DNA-Directed RNA Polymerase III Subunit RPC5 (RPC5) Antibody

20-abx119440
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA-Directed RNA Polymerase III Subunit RPC9 (RPC9) Antibody

20-abx148121
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA-Directed RNA Polymerase III Subunit RPC9 (CRCP) Antibody

20-abx333844
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA-directed RNA polymerase III subunit RPC10 Antibody (HRP)

20-abx108189
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA-Directed RNA Polymerase III Subunit RPC8 (RPC8) Antibody

abx032349-400ul 400 ul
EUR 627.6

DNA-Directed RNA Polymerase III Subunit RPC8 (RPC8) Antibody

abx032349-80l 80 µl
EUR 343.2

DNA-Directed RNA Polymerase III Subunit RPC2 (RPC2) Antibody

20-abx014433
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA-Directed RNA Polymerase III Subunit RPC4 (RPC4) Antibody

20-abx014435
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA-Directed RNA Polymerase III Subunit RPC8 (RPC8) Antibody

20-abx014436
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

PI 3 Kinase catalytic subunit alpha Antibody

48751-100ul 100ul
EUR 399.6

PI 3 Kinase catalytic subunit alpha Antibody

48751-50ul 50ul
EUR 286.8

DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody

abx117209-100ug 100 ug
EUR 560.4

DNA-Directed RNA Polymerase II Subunit RPB7 (POLR2G) Antibody

abx026827-400ul 400 ul
EUR 627.6

DNA-Directed RNA Polymerase II Subunit RPB7 (POLR2G) Antibody

abx026827-80l 80 µl
EUR 343.2

DNA-Directed RNA Polymerase II Subunit RPB7 (POLR2G) Antibody

abx031454-400ul 400 ul
EUR 627.6

DNA-Directed RNA Polymerase II Subunit RPB7 (POLR2G) Antibody

abx031454-80l 80 µl
EUR 343.2

DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody

abx038099-100ug 100 ug
EUR 469.2

DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody

20-abx126389
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA-Directed RNA Polymerase II Subunit RPB7 (POLR2G) Antibody

abx433156-200ul 200 ul
EUR 460.8

DNA-Directed RNA Polymerase II Subunit RPB7 (POLR2G) Antibody

20-abx006002
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

The cassettes contained the journalist genes, the luciferase of photine pyral (LUC) or an improved green fluorescent protein, under the control of the human cytomegalovirus promoter, immediate and early genes. In the in vitro system, the efficiency of the circular plasmid was 2.5 times greater than that of the PCR-amplified fragment. The effect of mutations in the expression cassette on the efficiency of the transgene expression in the PCR-amplified fragment was evaluated quantitatively. The mutations generated after 25 amplification cycles with the TAQ DNA the polymerase decreased the activity of the luciferase in cells transfected from 65 to 85%.

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