Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Trial chain tests of the three duplex transcription of the three duplex transcription (real-time RT-PCR) transcription based on Taqman chemistry, have been developed for simultaneous detection and the specific quantization of the virus Apple chlorotic leaves (ACLSV), plum pox virus (PPV), Prunus NecRotic Ringspot Virus (PNRSV), DNARE Virus (PDV), Latent Mosaic Virus (PLMVD) (PLMVD) and Fruit Phytoplasma European stone (Esfy), which are considered among the most important pathogens affecting stone fruit trees. The RT-PCR (RT-QPCR) quantitative analyzes have been optimized using RNA transcripts (plasmid linearized has been used for optimizing the determination of the esfy phytoplasm) of known concentrations. No difference in sensitivity was recorded between the duplex and RT-QPCR analyzes of RT-QPCR. The amplification efficiency of the recto-95.8% tests is 91.1 to 95.8%, while the linear quantization range was between 20 and 2 x 107 transcripts of linearized plasmid for PLMVD and phytoplasma esfy, 40 to 4 x 107 RNA transcripts for ACLSV, PPV and PDV and 102 to 108 RNA transcripts for PUNSV, respectively

Duplex RT-QPCR assays, which have been validated using all the two isolates characterized by all pathogens and field samples of Prunus species in northern Greece, have presented a wide range of detection . Overall, developed methods include useful tools that can be applied to simultaneous and reliable detection of transmissible transmissible pathogens in Prunus spp certification programs. Diarrheal diseases account for more than 50% of foodborne diseases around the world, the majority of which occur in infants and young children. The traditional bacterial detection method is complex and takes a long time; Therefore, it is necessary to establish a fast and practical detection method that can detect multiple pathogens simultaneously.

In this study, we have developed a set of five PCR dosages SYBR GREEN I Time-time multiplex to simultaneously detect 15 common pathical agents based on the non-dimeric system assisted by homo-tag. These analyzes efficiently reduce the formation of dimeric primer and improve the stability, uniformity and efficiency of the amplification of multiplex PCR.

The role of polymorphisms Genes of the vitamin D receptor in the risk of colorectal cancer

Vitamin D deficiency has been associated with an increase in the risk of incidence and mortality of colorectal cancer (CRC). The median vitamin D its action through the binding of the vitamin D receiver (VDR) and the polymorphisms of the VDR could explain these reverse associations. The objective of the study was the Survey of the relevance of RS731236; Thermus Aquaticus I (Taqi), ; Acetobacter Pasteurianus Sub. Pasteurianus I, Bacillus Stearothermophilus I (BSMI) Polymorphisms of the VDR gene with colorectal carcinogenesis (CRC) and progression. The peripheral blood was obtained from 397 patients with staging II / III (n = 202) and step IV (n = 195) and IV (n = 195). In addition, samples of 100 healthy donors and 40 patients with adenomatic polyps were also included as control groups.

 

 

Genotyping in patient and control samples was carried out using polymorphisms of fragmentation length of the reaction of the polymerase reaction (PCR-RFLP). An important association has been revealed between the four polymorphisms and cancer. Individuals with homozygous mutant genotypes (TT, AA, FF or BB) were more sensitive to the disease (p <0.001). All detected mutant genotypes have also been significantly associated with Phase IV (p <0.001), resulting in a significant decrease in survival (p <0.001). In addition, the four polymorphisms have been significantly associated with Krasten mutations (Kirsten Ras oncogene) and genetic variants of the toll receptor (TLR2, TLR4 and TLR9).

 Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees
Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Evaluation of seven African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples.

 

The African porcelain fever virus (ASFV) is a complex dual-stranded DNA virus, responsible for a highly infectious and mortal disease in pigs and shores and for a significant deterioration in animal welfare. Over the past decade, the disease has expanded to several European and Asian countries, causing unprecedented dramatic economic losses in the pork industry. In the absence of vaccine, affected countries are based on confidence diagnostic tests and test policies adapted to setting up control programs to combat disease.

In this study, we evaluated the sensitivity and specificity of seven real-time real-time PCR detection kits in the trade and three Taq polymerases on 300 samples of well-characterized boars collected in Belgium during the 2018 epidemic -2019. This study confirms that all commercial kits and two TAQ polymerases are suitable for ASFV detection in diagnostic laboratories. In addition, the use of endogenous controls is underlined when using field samples harvested on carcasses in an advanced decomposition step, in order to avoid falsely negative results. The functional efficacy of the expression cassettes integrated in a plasmid and a PCR-amplified fragment was relatively analyzed after the transitional transition transition in vitro or introduction into the development embryo of Danio Rerio.

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

20-abx338574
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

20-abx330112
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx331383-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

20-abx333842
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx114590
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx004069
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon Catalytic Subunit A (POLE1) Antibody

20-abx014425
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx327785
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (HRP)

20-abx335400
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (FITC)

20-abx335401
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (Biotin)

20-abx335402
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (HRP)

20-abx335409
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (FITC)

20-abx335410
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (Biotin)

20-abx335411
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx334340
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx236606-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (HRP)

20-abx335391
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (FITC)

20-abx335392
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (Biotin)

20-abx335393
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Epstein-Barr virus DNA polymerase catalytic subunit (BALF5)

1-CSB-EP355978EFA
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 39.2 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Epstein-Barr virus DNA polymerase catalytic subunit (BALF5) ,partial expressed in E.coli

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx011465-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

20-abx325215
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx331357-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

Human DNA polymerase delta catalytic subunit, POLD1 ELISA KIT

ELI-08845h 96 Tests
EUR 824

Mouse DNA polymerase delta catalytic subunit, Pold1 ELISA KIT

ELI-08846m 96 Tests
EUR 865

Mouse DNA polymerase zeta catalytic subunit, Rev3l ELISA KIT

ELI-09399m 96 Tests
EUR 865

Mouse DNA polymerase alpha catalytic subunit, Pola1 ELISA KIT

ELI-47649m 96 Tests
EUR 865

Human DNA polymerase zeta catalytic subunit, REV3L ELISA KIT

ELI-47651h 96 Tests
EUR 824

Human DNA polymerase alpha catalytic subunit, POLA1 ELISA KIT

ELI-32247h 96 Tests
EUR 824

Bovine DNA polymerase delta catalytic subunit, POLD1 ELISA KIT

ELI-47805b 96 Tests
EUR 928

Mouse DNA polymerase epsilon catalytic subunit A, Pole ELISA KIT

ELI-08849m 96 Tests
EUR 865

Human DNA polymerase epsilon catalytic subunit A, POLE ELISA KIT

ELI-27683h 96 Tests
EUR 824

Human DNA Polymerase Delta 1, Catalytic Subunit (POLD1) ELISA Kit

abx382340-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-48T 48T
EUR 332
  • The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-5platesof96wells 5 plates of 96 wells
EUR 2115
  • The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-96T 96T
EUR 539
  • The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-48T 48T
EUR 332
  • The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-5platesof96wells 5 plates of 96 wells
EUR 2115
  • The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-96T 96T
EUR 539
  • The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human DNA Polymerase Delta, catalytic subunit (Pol D) Control/blocking peptide

DNPD11-P 100 ug
EUR 164

Rabbit Anti-Human DNA Polymerase Delta, catalytic subunit (Pol D) antiserum

DNPD11-S 100 ul
EUR 457

DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PKcs) Antibody

20-abx008825
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PK) Antibody

20-abx013061
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PKcs) Antibody

abx232474-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-100ug

QP7276-ec-100ug 100ug
EUR 707

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-10ug

QP7276-ec-10ug 10ug
EUR 326

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-1mg

QP7276-ec-1mg 1mg
EUR 2303

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-200ug

QP7276-ec-200ug 200ug
EUR 1115

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-500ug

QP7276-ec-500ug 500ug
EUR 1514

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-50ug

QP7276-ec-50ug 50ug
EUR 435

Recombinant Leishmania Donovani DNA polymerase alpha catalytic subunit Protein (aa 1-1339)

VAng-Wyb7419-inquire inquire Ask for price
Description: Leishmania Donovani DNA polymerase alpha catalytic subunit, partial, recombinant protein.

Recombinant Trypanosoma Brucei DNA polymerase alpha catalytic subunit Protein (aa 1-1339)

VAng-Wyb8056-inquire inquire Ask for price
Description: Trypanosoma Brucei brucei DNA polymerase alpha catalytic subunit protein, partial, recombinant protein.

DNA Polymerase Delta Subunit 4 (POLD4) Antibody

20-abx005858
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Delta Subunit 4 (POLD4) Antibody

20-abx217846
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

Polymerase (Dna Directed), alpha 2 (70Kd Subunit) Antibody

20-abx114588
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

20-abx114591
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Polymerase (Dna Directed), epsilon 3 (P17 Subunit) Antibody

20-abx114592
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 4, Accessory Subunit (POLE4) Antibody

20-abx135826
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

abx034739-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

abx034739-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx034852-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx034852-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx036036-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

abx033322-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

abx033322-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

20-abx006001
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

20-abx004960
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

20-abx005465
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

abx028767-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

abx028767-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

20-abx323922
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 4, Accessory Subunit (POLE4) Antibody

20-abx325247
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

20-abx327451
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Epsilon 2, Accessory Subunit (POLE2) Antibody

20-abx217847
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

20-abx338731
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

20-abx334271
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Delta 2, Accessory Subunit (POLD2) Antibody

abx236607-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

DNA Polymerase Delta 3, Accessory Subunit (POLD3) Antibody

abx236608-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

DNA Polymerase Epsilon 3, Accessory Subunit (POLE3) Antibody

abx236610-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Anti-DNA polymerase? p66 subunit antibody monoclonal (2A1C11)

70-056 250ug Ask for price
Description: The Anti-DNA polymerase? p66 subunit antibody monoclonal (2A1C11) is available in Europe and for worldwide shipping via Gentaur.

Recombinant human DNA polymerase delta subunit 2

P2733 100ug Ask for price
  • Uniprot ID: P49005
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human DNA polymerase delta subunit 2

Recombinant human DNA polymerase subunit gamma-1

P2838 100ug Ask for price
  • Uniprot ID: P54098
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human DNA polymerase subunit gamma-1

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

AF0148 200ul
EUR 304
Description: DNA Polymerase beta antibody detects endogenous levels of total DNA Polymerase beta.

DNA Polymerase beta Antibody

20-abx008852
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

DNA Polymerase beta Antibody

20-abx013060
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 438

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 244
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 390

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 252

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 187

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 252

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 187

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 252

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 187

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 252

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 187

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 333

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 239

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 252

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 187

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 390

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

Mouse DNA-dependent protein kinase catalytic subunit (Prkdc)

1-CSB-EP018714MO
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 34.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse DNA-dependent protein kinase catalytic subunit(Prkdc),partial expressed in E.coli

DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody

20-abx126389
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody

abx117209-100ug 100 ug
EUR 467
  • Shipped within 5-10 working days.

DNA-Directed RNA Polymerase III Subunit RPC5 (RPC5) Antibody

20-abx119440
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA-Directed RNA Polymerase III Subunit RPC10 (POLR3K) Antibody

20-abx109277
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA-Directed RNA Polymerase III Subunit RPC3 (POLR3C) Antibody

20-abx109278
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA-directed RNA polymerase III subunit RPC10 Antibody (HRP)

20-abx108189
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA-directed RNA polymerase III subunit RPC3 Antibody (HRP)

20-abx108190
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody

20-abx001719
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA-Directed RNA Polymerase I Subunit RPA43 (TWISTNB) Antibody

abx145950-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

The cassettes contained the journalist genes, the luciferase of photine pyral (LUC) or an improved green fluorescent protein, under the control of the human cytomegalovirus promoter, immediate and early genes. In the in vitro system, the efficiency of the circular plasmid was 2.5 times greater than that of the PCR-amplified fragment. The effect of mutations in the expression cassette on the efficiency of the transgene expression in the PCR-amplified fragment was evaluated quantitatively. The mutations generated after 25 amplification cycles with the TAQ DNA the polymerase decreased the activity of the luciferase in cells transfected from 65 to 85%.

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