Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Trial chain tests of the three duplex transcription of the three duplex transcription (real-time RT-PCR) transcription based on Taqman chemistry, have been developed for simultaneous detection and the specific quantization of the virus Apple chlorotic leaves (ACLSV), plum pox virus (PPV), Prunus NecRotic Ringspot Virus (PNRSV), DNARE Virus (PDV), Latent Mosaic Virus (PLMVD) (PLMVD) and Fruit Phytoplasma European stone (Esfy), which are considered among the most important pathogens affecting stone fruit trees. The RT-PCR (RT-QPCR) quantitative analyzes have been optimized using RNA transcripts (plasmid linearized has been used for optimizing the determination of the esfy phytoplasm) of known concentrations. No difference in sensitivity was recorded between the duplex and RT-QPCR analyzes of RT-QPCR. The amplification efficiency of the recto-95.8% tests is 91.1 to 95.8%, while the linear quantization range was between 20 and 2 x 107 transcripts of linearized plasmid for PLMVD and phytoplasma esfy, 40 to 4 x 107 RNA transcripts for ACLSV, PPV and PDV and 102 to 108 RNA transcripts for PUNSV, respectively

Duplex RT-QPCR assays, which have been validated using all the two isolates characterized by all pathogens and field samples of Prunus species in northern Greece, have presented a wide range of detection . Overall, developed methods include useful tools that can be applied to simultaneous and reliable detection of transmissible transmissible pathogens in Prunus spp certification programs. Diarrheal diseases account for more than 50% of foodborne diseases around the world, the majority of which occur in infants and young children. The traditional bacterial detection method is complex and takes a long time; Therefore, it is necessary to establish a fast and practical detection method that can detect multiple pathogens simultaneously.

In this study, we have developed a set of five PCR dosages SYBR GREEN I Time-time multiplex to simultaneously detect 15 common pathical agents based on the non-dimeric system assisted by homo-tag. These analyzes efficiently reduce the formation of dimeric primer and improve the stability, uniformity and efficiency of the amplification of multiplex PCR.

The role of polymorphisms Genes of the vitamin D receptor in the risk of colorectal cancer

Vitamin D deficiency has been associated with an increase in the risk of incidence and mortality of colorectal cancer (CRC). The median vitamin D its action through the binding of the vitamin D receiver (VDR) and the polymorphisms of the VDR could explain these reverse associations. The objective of the study was the Survey of the relevance of RS731236; Thermus Aquaticus I (Taqi), ; Acetobacter Pasteurianus Sub. Pasteurianus I, Bacillus Stearothermophilus I (BSMI) Polymorphisms of the VDR gene with colorectal carcinogenesis (CRC) and progression. The peripheral blood was obtained from 397 patients with staging II / III (n = 202) and step IV (n = 195) and IV (n = 195). In addition, samples of 100 healthy donors and 40 patients with adenomatic polyps were also included as control groups.

 

 

Genotyping in patient and control samples was carried out using polymorphisms of fragmentation length of the reaction of the polymerase reaction (PCR-RFLP). An important association has been revealed between the four polymorphisms and cancer. Individuals with homozygous mutant genotypes (TT, AA, FF or BB) were more sensitive to the disease (p <0.001). All detected mutant genotypes have also been significantly associated with Phase IV (p <0.001), resulting in a significant decrease in survival (p <0.001). In addition, the four polymorphisms have been significantly associated with Krasten mutations (Kirsten Ras oncogene) and genetic variants of the toll receptor (TLR2, TLR4 and TLR9).

 Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees
Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees

Evaluation of seven African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples.

 

The African porcelain fever virus (ASFV) is a complex dual-stranded DNA virus, responsible for a highly infectious and mortal disease in pigs and shores and for a significant deterioration in animal welfare. Over the past decade, the disease has expanded to several European and Asian countries, causing unprecedented dramatic economic losses in the pork industry. In the absence of vaccine, affected countries are based on confidence diagnostic tests and test policies adapted to setting up control programs to combat disease.

In this study, we evaluated the sensitivity and specificity of seven real-time real-time PCR detection kits in the trade and three Taq polymerases on 300 samples of well-characterized boars collected in Belgium during the 2018 epidemic -2019. This study confirms that all commercial kits and two TAQ polymerases are suitable for ASFV detection in diagnostic laboratories. In addition, the use of endogenous controls is underlined when using field samples harvested on carcasses in an advanced decomposition step, in order to avoid falsely negative results. The functional efficacy of the expression cassettes integrated in a plasmid and a PCR-amplified fragment was relatively analyzed after the transitional transition transition in vitro or introduction into the development embryo of Danio Rerio.

DNA Polymerase Catalytic Subunit (BALF5) Antibody (HRP)

abx319918-50l 50 µl
EUR 162.5

DNA Polymerase Catalytic Subunit (BALF5) Antibody (FITC)

abx319919-100l 100 µl
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DNA Polymerase Catalytic Subunit (BALF5) Antibody (FITC)

abx319919-50l 50 µl
EUR 162.5

DNA Polymerase Catalytic Subunit (BALF5) Antibody (Biotin)

abx319920-100l 100 µl
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DNA Polymerase Catalytic Subunit (BALF5) Antibody (Biotin)

abx319920-50l 50 µl
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DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

20-abx338574
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DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

20-abx005838
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DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

abx005838-100g 100 µg
EUR 175

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

abx028437-400l 400 µl
EUR 518.75

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

abx028437-80l 80 µl
EUR 281.25

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

abx338574-100l 100 µl
EUR 250

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody

abx338574-50l 50 µl
EUR 162.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

20-abx333842
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DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

20-abx330112
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DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx331383-100ul 100 ul
EUR 510

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx330112-100g 100 µg Ask for price

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx330112-20g 20 µg
EUR 187.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx330112-50g 50 µg
EUR 250

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx333842-100g 100 µg
EUR 362.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx333842-20g 20 µg
EUR 162.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody

abx333842-50g 50 µg
EUR 250

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx327785
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DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx334340
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DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (HRP)

20-abx335409
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DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx236606-100ug 100 ug
EUR 577.2

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx114590
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DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

20-abx004069
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DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx236606-100g 100 µg
EUR 350

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx114590-100l 100 µl
EUR 612.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx327785-100g 100 µg
EUR 250

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx327785-50g 50 µg
EUR 187.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx334340-100g 100 µg
EUR 362.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx334340-20g 20 µg
EUR 162.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody

abx334340-50g 50 µg
EUR 250

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (HRP)

abx335409-100g 100 µg
EUR 362.5

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (HRP)

abx335409-20g 20 µg
EUR 162.5

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (HRP)

abx335409-50g 50 µg
EUR 250

DNA polymerase epsilon catalytic subunit A (POL2) Antibody

abx344147-100l 100 µl
EUR 350

DNA polymerase epsilon catalytic subunit A (POL2) Antibody

abx344147-50l 50 µl
EUR 250

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (FITC)

20-abx335410
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  • 100 ug
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DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (FITC)

abx335410-100g 100 µg
EUR 362.5

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (FITC)

abx335410-20g 20 µg
EUR 162.5

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (FITC)

abx335410-50g 50 µg
EUR 250

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (HRP)

20-abx335400
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DNA Polymerase Epsilon Catalytic Subunit A (POLE1) Antibody

20-abx014425
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DNA Polymerase Epsilon Catalytic Subunit A (POLE1) Antibody

abx014425-100g 100 µg
EUR 43.75

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (HRP)

abx335400-100g 100 µg
EUR 362.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (HRP)

abx335400-20g 20 µg
EUR 162.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (HRP)

abx335400-50g 50 µg
EUR 250

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (FITC)

20-abx335401
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DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (Biotin)

20-abx335411
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DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (FITC)

abx335401-100g 100 µg
EUR 362.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (FITC)

abx335401-20g 20 µg
EUR 162.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (FITC)

abx335401-50g 50 µg
EUR 250

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (Biotin)

abx335411-100g 100 µg
EUR 362.5

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (Biotin)

abx335411-20g 20 µg
EUR 162.5

DNA Polymerase Gamma, Catalytic Subunit (POLG) Antibody (Biotin)

abx335411-50g 50 µg
EUR 250

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (Biotin)

20-abx335402
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  • 100 ug
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DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (Biotin)

abx335402-100g 100 µg
EUR 362.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (Biotin)

abx335402-20g 20 µg
EUR 162.5

DNA Polymerase Epsilon, Catalytic Subunit (POLE) Antibody (Biotin)

abx335402-50g 50 µg
EUR 250

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (HRP)

20-abx335391
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DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (HRP)

abx335391-100g 100 µg
EUR 362.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (HRP)

abx335391-20g 20 µg
EUR 162.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (HRP)

abx335391-50g 50 µg
EUR 250

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (FITC)

20-abx335392
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  • 100 ug
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DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (FITC)

abx335392-100g 100 µg
EUR 362.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (FITC)

abx335392-20g 20 µg
EUR 162.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (FITC)

abx335392-50g 50 µg
EUR 250

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (Biotin)

20-abx335393
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  • 100 ug
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DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (Biotin)

abx335393-100g 100 µg
EUR 362.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (Biotin)

abx335393-20g 20 µg
EUR 162.5

DNA Polymerase Delta 1, Catalytic Subunit (POLD1) Antibody (Biotin)

abx335393-50g 50 µg
EUR 250

DNA polymerase δ Catalytic Subunit/POLD1 Rabbit pAb

E2382673 100ul
EUR 225
Description: Available in various conjugation types.

Epstein-Barr virus DNA polymerase catalytic subunit (BALF5)

1-CSB-EP355978EFA
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Description: Recombinant Epstein-Barr virus DNA polymerase catalytic subunit (BALF5) ,partial expressed in E.coli

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

20-abx325215
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REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx331357-100ul 100 ul
EUR 510

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx011465-100ug 100 ug
EUR 526.8

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx325215-100g 100 µg
EUR 250

REV3 Like, DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) Antibody

abx325215-50g 50 µg
EUR 187.5

Rat DNA Polymerase Gamma, Catalytic Subunit (POLG) ELISA Kit

abx510939-96tests 96 tests
EUR 687.5

Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

EK8395 96Т
EUR 799

Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

AE26731RA-48Tests 48 Tests
EUR 325
Description: Rat (Rattus norvegicus)

Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

AE26731RA-96Tests 96 Tests
EUR 610
Description: Rat (Rattus norvegicus)

Human DNA polymerase zeta catalytic subunit, REV3L ELISA KIT

ELI-47651h 96 Tests
EUR 988.8

Mouse DNA polymerase zeta catalytic subunit, Rev3l ELISA KIT

ELI-09399m 96 Tests
EUR 1038

Human DNA polymerase zeta catalytic subunit (REV3L) ELISA Kit

abx510958-96tests 96 tests
EUR 687.5

Mouse DNA polymerase zeta catalytic subunit (REV3L) ELISA Kit

abx510959-96tests 96 tests
EUR 687.5

Human DNA polymerase alpha catalytic subunit, POLA1 ELISA KIT

ELI-32247h 96 Tests
EUR 988.8

Mouse DNA polymerase alpha catalytic subunit, Pola1 ELISA KIT

ELI-47649m 96 Tests
EUR 1038

Human DNA polymerase delta catalytic subunit, POLD1 ELISA KIT

ELI-08845h 96 Tests
EUR 988.8

Mouse DNA polymerase delta catalytic subunit, Pold1 ELISA KIT

ELI-08846m 96 Tests
EUR 1038

Mouse DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

EK11448 96Т
EUR 799

Human DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

AE26732HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)

Human DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

AE26732HU-96Tests 96 Tests
EUR 610
Description: Human (Homo sapiens)

Mouse DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

AE60086MO-48Tests 48 Tests
EUR 325
Description: Mouse (Mus musculus)

Mouse DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

AE60086MO-96Tests 96 Tests
EUR 610
Description: Mouse (Mus musculus)

Mouse DNA polymerase delta catalytic subunit (POLD1) ELISA Kit

abx510911-96tests 96 tests
EUR 687.5

Mouse DNA polymerase alpha catalytic subunit (POLA1) ELISA Kit

abx510944-96tests 96 tests
EUR 687.5

Bovine DNA polymerase delta catalytic subunit, POLD1 ELISA KIT

ELI-47805b 96 Tests
EUR 1113.6

Rat DNA Polymerase Delta 1, Catalytic Subunit (POLD1) ELISA Kit

abx510912-1096tests 10 × 96 tests Ask for price

Rat DNA Polymerase Delta 1, Catalytic Subunit (POLD1) ELISA Kit

abx510912-596tests 5 × 96 tests Ask for price

Rat DNA Polymerase Delta 1, Catalytic Subunit (POLD1) ELISA Kit

abx510912-96tests 96 tests
EUR 687.5

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)

KTE100462-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human DNA Polymerase Delta 1, Catalytic Subunit (POLD1) ELISA Kit

abx382340-96tests 96 tests
EUR 1093.2

Mouse DNA polymerase epsilon catalytic subunit A, Pole ELISA KIT

ELI-08849m 96 Tests
EUR 1038

Human DNA polymerase epsilon catalytic subunit A, POLE ELISA KIT

ELI-27683h 96 Tests
EUR 988.8

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)

KTE71449-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human DNA polymerase epsilon catalytic subunit A (POLE) ELISA Kit

abx510923-96tests 96 tests
EUR 687.5

Mouse DNA polymerase epsilon catalytic subunit A (POLE) ELISA Kit

abx510924-96tests 96 tests
EUR 687.5

Rabbit polyclonal antibody to DNA pol delta cat (polymerase (DNA directed), delta 1, catalytic subunit 125kDa)

TA308522 100 µl Ask for price

Rabbit Anti-Human DNA Polymerase Delta, catalytic subunit (Pol D) antiserum

DNPD11-S 100 ul
EUR 548.4

Recombinant Epstein-Barr virus DNA polymerase catalytic subunit (BALF5), partial

CSB-EP355978EFA 3213 mg Ask for price

Recombinant Epstein-Barr virus DNA polymerase catalytic subunit (BALF5) ,partial

AP71109 1mg
EUR 2826

Human DNA Polymerase Delta, catalytic subunit (Pol D) Control/blocking peptide

DNPD11-P 100 ug
EUR 196.8

Pola1 (untagged) - Rat polymerase (DNA directed), alpha 1, catalytic subunit (Pola1)

RN217636 10 µg Ask for price

POLA1 (untagged)-Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1)

SC304453 10 µg Ask for price

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-10ug

QP7276-ec-10ug 10ug
EUR 391.2

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-1mg

QP7276-ec-1mg 1mg
EUR 2763.6

Recombinant Epstein-Barr DNA polymerase catalytic subunit Protein, His-SUMO, E.coli-50ug

QP7276-ec-50ug 50ug
EUR 522

The cassettes contained the journalist genes, the luciferase of photine pyral (LUC) or an improved green fluorescent protein, under the control of the human cytomegalovirus promoter, immediate and early genes. In the in vitro system, the efficiency of the circular plasmid was 2.5 times greater than that of the PCR-amplified fragment. The effect of mutations in the expression cassette on the efficiency of the transgene expression in the PCR-amplified fragment was evaluated quantitatively. The mutations generated after 25 amplification cycles with the TAQ DNA the polymerase decreased the activity of the luciferase in cells transfected from 65 to 85%.

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