Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR)

Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR)

The methodologies of the directed evolution benefit from quantitative reading quantitatively genotype at the phenotype. We have therefore designed a method flowing from protein-peptide interactions to dynamic reading provided by a modified polymerase DNA. The melting of a processivity clamping protein on a polymerase thermostable nucleic acid allows a polymerase activity and an amplification of the DNA in high prohibition salt buffers. Here, we recap this phenotype by indirectly coupling the SSO7D processing clamp to the TAQ DNA polymerase via a respective fusion to a pair of high affinity peptide proteins and thermostable interaction. Escherichia coli cells co-expressing peptide-peptide-peptide pairs can be used directly in polymerase chain reactions to determine the relative interaction resistors by measuring amplic yields. The conditional activity of the polymerase is also used to connect the genotype to the phenotype of the interactive peptide pairs co-expressed in E. coli using the phased evolution platform for the compartmentalized automatic replication.

We validate this approach, called the replication with two hybrid compartmentalized, by selecting for the high affinity peptides that bind two models of model proteins: Spycacher and the large fragment of Nanoluc Luciferase. We demonstrate more co-evolution directed by hiking both protein and peptide components of the SpycCatcher-Spytag pair and by co-selecting for functional interactive variants. An important toxin-antitoxin (TA) HOK / Sok system, encoded by the plasmid R1 of Escherichia coli, is involved in the wake of post-segregation cells that have lost the plasmid. The deadly properties of HOK protein have been used for environmental containment of microbes and the development of potential vaccine candidates. We aim to demonstrate the powerful antimicrobial property of 19 fragment of the HOK peptide amino acid (AA) terminal.

This has been accomplished by designing a conditional suicide system based on HOK gene expression, cloned in an anhydroteracyclacin (ATC) inducible vector – PASK75. Thermal shocks and electroporation were used for the transformation of Escherichia coli cells and Vibrio Cholerae respectively. The minimum induction concentration (medium C) of the ATC, determined by analyzing the expression of the green fluorescent protein cloned separately in the PASK75 vector, was 30 ng / ml. As the HOK gene has been synthesized by Novo (using the reaction of the recombinant polymerase chain) in our study, various random size HOK fragments were generated (as a result of the possible nature of Taq. polymerase).

 

Building better DNA polymerases: Engineering Replication of Expanded Genetic Alphabets

 

DNA polymerases are now used in all scientific research, biotechnology and medicine, in part for their ability to interact with non-natural forms of DNA created by synthetic biologists. Here, especially, natural DNA polymerases often do not have “performance specifications” needed for processing technologies. This creates a need for rational (or semi-rational) scientific engineering engineering in order to identify variants that reproduce non-natural basic pairs (UBP), non-natural backbones, tags or other features evolutionually new from the Non-natural DNA. In this review, we provide a brief overview of the chemistry and properties of the replica DNA polymerases and their evolved variants, with a focus on the Klenow fragment of Taq DNA polymerase (Klentaq).

We describe studies of structural, enzymatic and comparative molecular dynamics of wild-type variants and klentaq, complex with natural or non-canonic substrates. The combination of these methods makes it possible to understand how the specific amino acid substitutions remote from the active site in a variant of the Klentaq DNA polymerase (ZP Klentaq) contribute to its ability to reproduce non-natural basic pairs (UBP). With improved efficiency compared to Klentaq. This approach can therefore be used to guide any future rational engineering of replicative DNA polymerases.

 Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR)
Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR)

Evaluation of the comparability of different methods of DNA extraction and amplification in microbial community profiling of intestines

 

Automated and broadband technologies are becoming increasingly common in microbiome studies to reduce costs and increase efficiency. However, in microbiome studies, small differences in methodology – including storage conditions, wet laboratory methods, sequencing platforms and data analysis – can affect the reproducibility and comparability of data between the studies.

The effects of broadband methods have been limited, including microfluidic PCR technologies. In this article, we compare two extraction methods (the mini Qiaamp DNA stool kit and the Mobio PowerSoil DNA insulation kit), two Taq polymerase enzymes (Mytaq HS Red Mix and Lock II PCR Tougymix) , two sets of primers (V3-V4 and V4 -V5) and two amplification methods (a two-step PCR protocol and a preparation of the amplicon library on the fluidigm access network system that allows multiplexing automated primers). The profiles of the Gut microbial community have been considerably affected by all variables. Although there has been no significant difference in alpha diversity measured between the two extraction methods, there was an effect of the extraction method on the community composition measured by unrestricted unifruc distances. The amplification method and primers have had a significant effect on alpha diversity and community composition.

DNA Polymerase ζ Conjugated Antibody

C33602 100ul
EUR 476.4

DNA Polymerase Antibody

E11-11836C 100μg
EUR 225
Description: Available in various conjugation types.

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 399.6

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 286.8

Anti-Taq DNA Polymerase Antibody

E8EM1701-49 100ul
EUR 275
Description: Available in various conjugation types.

anti- DNA polymerase beta antibody

FNab02432 100µg
EUR 606.3
Description: Antibody raised against DNA polymerase beta

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

E11-0173C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E11-10173C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E11-10322C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E033357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E033611 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E033665 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase λ Antibody

E034098 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E19-3082 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Anti-Taq DNA Polymerase Antibody [3C3]

EM1701-49 100ul
EUR 210
Description: Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

Anti-DNA Polymerase Antibody

E38A6546 100ug/100ul
EUR 225
Description: Available in various conjugation types.

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA Polymerase beta Antibody

20-abx008852
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

AF0148-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

AF0148-200ul 200ul
EUR 350

DNA Polymerase zeta Antibody

E38PA4807 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase iota Antibody

E38PA5564 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

DF6552 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase beta Antibody

DF6552-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

DF6552-200ul 200ul
EUR 350

DNA Polymerase beta Antibody

AF0148 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase beta Antibody

E18-0148-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

E18-0148-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

AF5235-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

AF5235-200ul 200ul
EUR 350

DNA Polymerase theta Antibody

DF13563-100ul 100ul
EUR 280

DNA Polymerase theta Antibody

DF13563-200ul 200ul
EUR 350

DNA Polymerase alpha Antibody

DF3082-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

DF3082-200ul 200ul
EUR 350

DNA Polymerase alpha Antibody

AF5235 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase theta Antibody

DF13563 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase alpha Antibody

DF3082 100ul
EUR 280
Description: Human

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

Anti-POLK/Dna Polymerase Kappa Antibody

A01987 100ug
EUR 518.4
Description: Goat Polyclonal POLK/Dna Polymerase Kappa Antibody. Validated in WB and tested in Human.

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 476.4

DNA Polymerase Mu (POLM) Antibody

abx236614-100ug 100 ug
EUR 661.2

DNA Polymerase Mu (POLM) Antibody

20-abx217851
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA Polymerase Mu (POLM) Antibody

20-abx141146
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase Nu (POLN) Antibody

abx028202-400ul 400 ul
EUR 627.6

DNA Polymerase Nu (POLN) Antibody

abx028202-80l 80 µl
EUR 343.2

DNA Polymerase Mu (POLM) Antibody

20-abx323923
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Eta (POLH) Antibody

abx117166-100ug 100 ug
EUR 560.4

DNA Polymerase Eta (POLH) Antibody

abx036483-100ug 100 ug
EUR 469.2

DNA Polymerase Eta (POLH) Antibody

20-abx001515
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Eta (POLH) Antibody

20-abx302606
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA polymerase eta Antibody / POLH

R32359 100 ug
EUR 356.15
Description: DNA polymerase eta (POLH), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

DNA Polymerase delta 3 antibody

70R-50825 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase delta 3 antibody

DNA Polymerase alpha 1 antibody

70R-50225 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase alpha 1 antibody

DNA Polymerase delta 1 antibody

70R-50227 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase delta 1 antibody

DNA Polymerase delta 3 Antibody

20-abx008311
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase delta 1 Antibody

20-abx008851
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase alpha 1 Antibody

20-abx008853
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase delta 3 Antibody

E38PA2506 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase gamma 2 Antibody

E38PA4161 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase alpha 1 Antibody

E38PA1906 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase delta 1 Antibody

E38PA1908 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase Iota (POLI) Antibody

abx236612-100ug 100 ug
EUR 577.2

DNA Polymerase Iota (POLI) Antibody

20-abx333843
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

abx036752-100ug 100 ug
EUR 469.2

DNA Polymerase Iota (POLI) Antibody

abx028115-400ul 400 ul
EUR 627.6

DNA Polymerase Iota (POLI) Antibody

abx028115-80l 80 µl
EUR 343.2

DNA Polymerase Iota (POLI) Antibody

20-abx323859
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

20-abx001585
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase iota Antibody / POLI

RQ4298 100 ug
EUR 356.15
Description: DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene. The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.

DNA polymerase beta Antibody / POLB

R31898 100 ug
EUR 356.15
Description: Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

DNA Polymerase beta Antibody / POLB

RQ5162 100ul
EUR 356.15
Description: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. [RefSeq]

DNA polymerase delta cat antibody

70R-12690 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase delta cat antibody

DNA polymerase delta p50 Antibody

DF13788-100ul 100ul
EUR 280

DNA polymerase delta p50 Antibody

DF13788-200ul 200ul
EUR 350

DNA polymerase delta p50 Antibody

DF13788 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase Kappa (POLK) Antibody

abx236613-100ug 100 ug
EUR 610.8

DNA Polymerase Kappa (POLK) Antibody

abx031322-400ul 400 ul
EUR 627.6

DNA Polymerase Kappa (POLK) Antibody

abx031322-80l 80 µl
EUR 343.2

DNA Polymerase Sigma (POLS) Antibody

abx029151-400ul 400 ul
EUR 627.6

DNA Polymerase Sigma (POLS) Antibody

abx029151-80l 80 µl
EUR 343.2

DNA Polymerase Kappa (POLK) Antibody

20-abx126383
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx126384
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx126385
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Theta (POLQ) Antibody

20-abx322965
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Kappa (POLK) Antibody

20-abx338646
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Theta (POLQ) Antibody

abx432614-200ul 200 ul
EUR 343.2

DNA Polymerase Kappa (POLK) Antibody

20-abx004686
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Theta (POLQ) Antibody

20-abx013320
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Anti-DNA polymerase eta Antibody

STJ502747 100 µg
EUR 571.2

DNA Polymerase Zeta (REV3L) Antibody

20-abx133309
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase epsilon 2 antibody

70R-50228 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase epsilon 2 antibody

Human DNA Polymerase beta Antibody

33292-05111 150 ug
EUR 313.2

DNA Polymerase epsilon 2 Antibody

20-abx008850
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase Zeta (REV3L) Antibody

20-abx013311
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase epsilon 2 Antibody

E38PA1909 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase Lambda (POLL) Antibody

20-abx217850
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA Polymerase Lambda (POLL) Antibody

abx332616-100ul 100 ul
EUR 510

DNA Polymerase Lambda (POLL) Antibody

20-abx013831
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase Lambda (POLL) Antibody

abx034579-400ul 400 ul
EUR 627.6

DNA Polymerase Lambda (POLL) Antibody

abx034579-80l 80 µl
EUR 343.2

DNA Polymerase Lambda (POLL) Antibody

20-abx328341
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Lambda (POLL) Antibody

abx430146-200ul 200 ul
EUR 460.8

DNA Polymerase Lambda (POLL) Antibody

20-abx005922
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Lambda (POLL) Antibody

20-abx301115
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase lambda Antibody / POLL

RQ4359 100 ug
EUR 356.15
Description: DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.

Anti-DNA Polymerase thet Antibody

A02205 100ul
EUR 476.4
Description: Rabbit Polyclonal DNA Polymerase thet Antibody. Validated in IHC and tested in Human.

Anti-DNA polymerase beta antibody

PAab02432 100 ug
EUR 426

Rabbit anti-DNA Polymerase mu Antibody

DL95067A-100ul 100 ul
EUR 270
Description: polmu; DNA-directed DNA/RNA polymerase mu; Pol Mu; Terminal transferase

Rabbit anti-DNA Polymerase mu Antibody

DL95067A-50ul 50 ul
EUR 189
Description: polmu; DNA-directed DNA/RNA polymerase mu; Pol Mu; Terminal transferase

The relative abundance of actinobacteria was significantly lowered when using the Mobio Kit amplification method or fluidigm, and the relative abundance of firmoles was lower when using the Qiagen Kit. Microbial community profiles based on fluidigm-generated amplicon libraries have not been comparable to those generated by more commonly used methods. Researchers should carefully consider limitations and biases that different methods of extraction and amplification can introduce their results. In addition, a deeper benchmarking of automated methods and multiplexing is required to determine the magnitude of the potential compromise between the quality and the amount of data.

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