Effect of surface hydrophobicity on activity of immobilized Taq DNA polymerase, its reusability and storage.

Effect of surface hydrophobicity on activity of immobilized Taq DNA polymerase, its reusability and storage.

Oriented methods and activity-preserved immobilization of biologically active proteins based on the concept of active-site masking and previously reported kinetic control. We extended our research and found that hydrophobic surfaces have a noticeable effect on the activity of the active-protected site move (PIM) Taq DNA polymerase in a mixture of assembled monolayer (SAM) is produced on the Au surface. Hydrophobic SAM created using 12-mercaptododecanoic acid and 1-heptanethiol.

Taq DNA polymerase activity generated is measured by PCR amplification and compared with previously reported values ​​obtained with the hydrophilic SAM. The maximum activity of the immobilized Taq DNA polymerase was achieved in 17.5% of 12-mercaptododecanoic acid and within 90 minutes of reaction time is higher than that obtained with the hydrophilic SAM; The maximum activity by 5% 12-mercaptododecanoic acid and at 10 minutes. To apply to a commercial level, moving enzymes have to be stable, reusable and can be stored. PIM stable Taq DNA polymerase attached to the surface and can be used for as many as 10 runs PCR comparable with enzyme-phase solution. Even after 56 days of storage at 4 ° C, the immobilized enzyme classified as 70% of the initial PIM enzyme activity. These data indicate that the PIM Taq DNA polymerase can be used for a variety of commercial applications.


DNA polymerases are enzymes that play an important role in DNA metabolism such as replication, repair, transcription, recombination, and chromosome segregation during mitosis. Here we report the isolation of a new iridoid (6-epi-catalpol, 2) and the per-O-acetyl-verbascoside (11) of the aerial parts of Buddleja cordobensis Grisebach (Buddlejaceae). Of the compound 2, we have obtained eight compounds with chemical transformations. This group of compounds at a concentration of 500μM tested against Taq DNA polymerase.

Compound 11 (per-O-acetyl-verbascoside) was the most active with IC50 of 1.21 ± 0.18μM; Compound 9, 2 and 8 are strong inhibitors with IC50 values ​​of 5:57 ± 0.70, 21.62 ± 0.22 and 78.13 ± 0.93μM, respectively. Compound 11 and 9 could be the structure of a new leader for the development of anticancer drugs chemotherapy and useful tools to investigate the activity of DNA polymerase.

 Effect of surface hydrophobicity on activity of immobilized Taq DNA polymerase, its reusability and storage.
Effect of surface hydrophobicity on activity of immobilized Taq DNA polymerase, its reusability and storage.

The stability of Taq DNA polymerase result of folding entropic penalty is reduced; identification of other thermophilic proteins with a similar folding thermodynamics.

The thermal stability of Taq DNA polymerase known, and is the basis for use in PCR. A comparison of the thermodynamic characterization of a large fragment of Taq domain (Klentaq) and E. coli (Klenow) of DNA polymerase has done by getting the Gibbs-Helmholtz stability curve full folding free energy (AG) versus temperature. This analysis provides temperature dependencies folding enthalpy and entropy (ΔH and ΔS), and heat capacity (ΔCp) fold.

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
EUR 127.2

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 710.4

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 276

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 393.6

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 196.8

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 314.4

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 490.8

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

L7051001 1000 unit
EUR 159

Taq DNA Polymerase

L7051200 200 unit
EUR 41

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

KD Plus DNA Polymerase Enzyme

abx071022-100U 100 U
EUR 292.8

KD Plus DNA Polymerase Enzyme

abx071022-1200U 1200 U
EUR 1078.8

KD Plus DNA Polymerase Enzyme

abx071022-200U 200 U
EUR 376.8

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 104.4

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase

S101 500 units
EUR 55.2

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 128.4

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 393.6

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 567

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 159

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

Recombinant Taq Dna Polymerase

27079804 EACH
EUR 117.6

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 639.6

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 620.4

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U each
EUR 272.4

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U each
EUR 126

Taq DNA Polymerase Polyclonal Antibody

7018-100 each
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T each
EUR 175.2

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

Human DNA Polymerase Beta Assay Kit Plus

DPB100KE 100 assays
EUR 448.28
Description: This product includes 0.800 ml of 10 x Buffer BP, 0.055 ml of 100x DNA template, 0.055 ml of 100 x dNTP mix, 0.055 ml of 100 x human DNA polymerase beta, 22 ml of Reagent U and 0.420 ml of 10 x fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format.

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 314.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 157.2

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-100 2x500 units
EUR 230.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-200 4x500 units
EUR 375.6

Human DNA Polymerase Gamma Assay Kit Plus

DPG100KE 100 assays
EUR 467.15
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 33 ul of 100 x human DNA polymerase gamma, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Human DNA Polymerase Alpha Assay Kit Plus

HDPA100KE1 100 assays
EUR 624.67
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP mix, 30 µl of 100 x human DNA polymerase alpha (4 µM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-01 125 U
EUR 145.2

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-02 500 U
EUR 210

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 355.2
Description: Minimum order quantity: 1 unit of 500EU

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1388.4

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 6540

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 176.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 273.6

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 420.65

KD Plus DNA Polymerase (with 2.5 mM dNTPs) Enzyme

abx071023-100U 100 U
EUR 292.8

KD Plus DNA Polymerase (with 2.5 mM dNTPs) Enzyme

abx071023-1200U 1200 U
EUR 1111.2

KD Plus DNA Polymerase (with 2.5 mM dNTPs) Enzyme

abx071023-200U 200 U
EUR 376.8

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 896.24

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 129.6

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Genorise® CLT Taq DNA Polymerase (1000 U)

GR108003 1000 U
EUR 199

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 228

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 399.6

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 286.8

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S111 500 units
EUR 63.6

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S112 5x500 units
EUR 148.8

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S128 20x500 units
EUR 487.2

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S129 100x500 units or as bulk
EUR 2142

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 198

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 360

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 699.6

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-100 100,000 units
EUR 3462

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-010 100 units
EUR 180

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3

P0330-020 2x100 units
EUR 286.8

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-050 5X100 units
EUR 465.6

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-250 25X100 units
EUR 2004

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-500 50X100 units
EUR 3591.6

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d1 125 U
EUR 147.6

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d2 500 U
EUR 213.6

Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit Plus-100

HCMV100KE 100 assays
EUR 444.91
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 33 ul of 100 x HCMV DNA polymerase, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 490.8

Reversible 1-min Hot Start Taq DNA Polymerase

P950 - Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase

P950L - Ask for price

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B01 10,000 units
EUR 1388.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B05 5x10,000 units
EUR 6540

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

H influenzae DNA Polymerase Assay Kit Plus (enzyme included)

DPA100KH 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x H. influenzae DNA polymerase III alpha subunit.

S. pneumoniae DNA Polymerase Assay Kit Plus (enzyme included)

DPA100KN 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x S. pneumoniae DNA polymerase III alpha subunit.

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 122.4

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1830

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 476.4

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-025 250 unit
EUR 200.4

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-050 2X250 unit
EUR 300

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-100 4X250 Unit
EUR 505.2

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-250 10X250 Unit
EUR 886.8

EZ Taq Polymerase (HotStart)

9K-005-0020 500U
EUR 245.83

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S113 2x100 rcs
EUR 79.2

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S114 10x100 rcs
EUR 231.6

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 231.6

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 428.4

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-050 500 units
EUR 198

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-102 25x500 units
EUR 1712.4

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-500 10X500 units
EUR 886.8

E. coli DNA polymerase Assay Kit Plus (E. coli DNA pol III alpha subunit included)

DPA100KE 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x E. coli DNA polymerase III alpha subunit.

Accuris Taq Polymerase, 1000 units

PR1000-1000 1 PC
EUR 167.1

Accuris Taq Polymerase, 6000 units

PR1000-6000 1 PC
EUR 853.3

Accuris Taq Polymerase, 500 units

PR1000-500 1 PC
EUR 103.6

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-500 500 units
EUR 262.8

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-502 2x500 units
EUR 424.8

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-510 10x500 units
EUR 1647.6

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-525 25x500 units
EUR 3624

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-550 50x500 units
EUR 6459.6

TaqFast DNA Polymerase (PCR)

G277 250 U (50 ul)
EUR 87.6

TaqFast DNA Polymerase (PCR)

G278 1000 U (200 ul)
EUR 160.8

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 575.22

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2465.38

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 384.17

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2878.8

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 1055.98

E. coli RNA polymerase Assay Kit Plus (E. coli RNA polymerase included)

RPA100KE 100 assays
EUR 467.15
Description: This product includes all the reagents in E. coli RNA Polymerase Assay Kit (Catalog number RPA100K) plus the enzyme, 33 ul of 100 x E. coli RNAP. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

T4 DNA Polymerase

M1211-100 each
EUR 385.2

Accuris Taq Polymerase, sample, 50 units

PR1000-S 1 PC
EUR 7.1

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

PFU DNA Polymerase

9003-2500 each
EUR 614.4

PFU DNA Polymerase

9003-500 each
EUR 216

Laq? DNA Polymerase

9004-2500 each
EUR 732

Laq? DNA Polymerase

9004-500 each
EUR 261.6

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

Human Mitochondrial RNA Polymerase Assay Kit Plus

MRPA100KE 100 assays
EUR 624.67
Description: This product includes 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x Human Mitochondrial RNA Polymerase, 33 ul of 100 x NTP mix, 330 ul of 10 x fluorescence dye.

If increased or enhanced non-covalent bonding in the original state is responsible for increasing the thermal stabilization of proteins, such as frequently asked questions, then folding enthalpy enhanced benefit should, in general, observed for thermophilic proteins. However, for Klenow-Klentaq homologous pairs, folding enthalpy (ΔHfold) of Klentaq far less profitable than Klenow at all temperatures. Instead, it was found that the free energy of folding extreme Klentaq (ΔGfold) comes from the entropic penalty significantly reduces the fold (ΔSfold). In addition, the heat capacity change at a similar fold for Klenow and Klentaq.

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