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Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.
DNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. They are used in various fields of science, especially as an important component for the in vitro synthesis of DNA, known as PCR. modern diagnostics, molecular biology and genetic engineering of DNA polymerase needs which demonstrate improved performance. This study aims to get NeqSSB-TaqS new DNA polymerase fusion of domain Stoffel Taq DNA and single-stranded DNA binding protein from Nanoarchaeum equitans as to significantly improve the properties of the DNA polymerase.
Stoffel Taq DNA coding sequence specific DNA polymerase and DNA-binding proteins Nanoarchaeum equitans (NeqSSB-like protein) fused. A new recombinant genes obtained were cloned into pET-30 Ek / LIC vector and introduced into E. coli for expression. Purified recombinant enzymes and enzymatic properties including the activity of DNA polymerase, PCR amplification level, thermostability, processivity and resistance to inhibitors, were tested. The results of the target protein around 18 mg / l after 24 hours of IPTG induction.
The specific activity of the polymerase is 2200 U / mg. Recombinant NeqSSB-TaqS exhibited a higher level extensions (1000 bp template 20 s), processivity (19 nt), thermostability (half-life 35 minutes at 95 ° C) and a higher tolerance for PCR inhibitors (0.3 to 1.25 % of whole blood, 0.84 to 13.5 ug heparin lactoferrin and 4.7 to 150 ng) of Taq DNA polymerase Stoffel. In addition, our research shows that NeqSSB-TaqS DNA polymerase has a high degree of flexibility in relation to the Mg 2+ ions (from 1 to 5 mM) and KCl or (NH4) 2SO4 salt (more than 60 mM and 40 mM, respectively ). NeqSSB-TaqS using DNA polymerase Taq DNA polymerase can not be a better choice in many applications of PCR.
Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.
dUTPs zwitterionic conjugated with Cy3 or Cy5 analog Fluorophore effective substrate for DNA amplification and labeling by Taq polymerase.
To develop the structural modifications dNTP compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dyes of different analog responsible and charge distribution throughout the fluorophore. Cy3- derivatives and commercial dUTP and Cy5-dUTP studied in Taq polymerase dependent polymerase chain reaction (PCR) and the primer extension reaction using a model template that contains one, two and three adjacent nucleotides adenine.
The relative amount of DNA is amplified and kinetic parameters Km and Vmax characterize grief labeled incorporation have been estimated using fluorescence measurements and analyzed. The dUTPs label with analog zwitterionic electroneutral of Cy3 or Cy5 fluorophores Taq polymerase is used by approximately one order of magnitude more effective than dUTPs analog labeled with a negatively charged of Cy3 or Cy5. Nucleotidyl transferase activity of Taq polymerase were also observed and resulted in the addition dumps electroneutral labeled with fluorophores or positively charged to the 3 ‘end of the DNA.
Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ200
Allele Biotech
200U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ500
Allele Biotech
500U
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3G HotStart Taq DNA Polymerase (5u/ul)
3GHST81
Bio Basic
5x500U
EUR 746.87
Taq DNA Polymerase (High Purity), 5u/ul
HTD0078
Bio Basic
5X200U
EUR 108
Taq DNA Polymerase
9001-2500
Biovision
EUR 512
Taq DNA Polymerase
9001-500
Biovision
EUR 191
Taq DNA Polymerase
L7051001
Biochain
1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Taq DNA Polymerase
L7051200
Biochain
200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Taq DNA Polymerase
BA00103
Cusabio
500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00104
Bioatlas
1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00105
Bioatlas
2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10101
Bioatlas
500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10102
Bioatlas
1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10103
Bioatlas
2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.
T4 DNA Polymerase, 5u/ul
BEP0061
Bio Basic
100U
EUR 128.31
Pfu DNA Polymerase, 5u/ul
B0093(D0090P)
Bio Basic
500U
EUR 137
Tsg DNA Polymerase, 5u/ul
D0081
Bio Basic
5X200U
EUR 93.5
Taq DNA Polymerase (PCR)
G008
ABM
5000 U (1.0 ml)
EUR 205
Taq DNA Polymerase (PCR)
G009
ABM
1000 U (200 ul)
EUR 87
Taq DNA Polymerase (PCR)
G126
ABM
10000 U (2 x 1.0 ml)
EUR 309
Champagne Taq DNA Polymerase
P122-d1
Vazyme
500 U (2.5 U/μl)
EUR 177
Champagne Taq DNA Polymerase
P122-d2
Vazyme
500 U (5 U/μl)
EUR 177
Champagne Taq DNA Polymerase
P122-d3
Vazyme
500 U (10 U/μl)
EUR 177
Taq Plus DNA Polymerase
P201-01
Vazyme
250 U
EUR 123
Taq Plus DNA Polymerase
P201-02
Vazyme
1000 U
EUR 178
Taq Plus DNA Polymerase
P201-03
Vazyme
3000 U
EUR 319
Hotstart Taq DNA Polymerase
L7052001
Biochain
1000 units
EUR 568
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Hotstart Taq DNA Polymerase
L7052250
Biochain
250 units
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Taq DNA Polymerase (500U)
9K-001-0001
Bio Basic
500U
EUR 174.41
Taq DNA Polymerase (3000U)
9K-001-0002
Bio Basic
3000U
EUR 485
Taq DNA Polymerase (6000U)
9K-001-0033
Bio Basic
6000U
EUR 1028.75
Taq DNA Polymerase (1000U)
9K-001-0035
Bio Basic
1000U
EUR 348.85
Taq DNA Polymerase (Recombinant)
abx073521-10000U
Abbexa
10000 U
EUR 592
Taq DNA Polymerase (Recombinant)
abx073521-1000U
Abbexa
1000 U
EUR 230
Taq DNA Polymerase (Recombinant)
abx073521-3000U
Abbexa
3000 U
EUR 328
Recombinant Taq DNA Polymerase
7-03634
CHI Scientific
1,000U
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Recombinant Taq DNA Polymerase
7-03635
CHI Scientific
3,000U
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Recombinant Taq DNA Polymerase
7-03636
CHI Scientific
10,000U
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Taq DNA polymerase (+dNTPs)
02-001
Sceti
200U
EUR 153
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (+dNTPs)
02-001-5
Sceti
5×200U
EUR 257
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (-dNTPs)
02-011
Sceti
200U
EUR 140
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (-dNTPs)
02-011-5
Sceti
5×200U
EUR 218
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Maximo Taq DNA Polymerase
S101
GeneOn
500 units
EUR 46
Maximo Taq DNA Polymerase
S102
GeneOn
5x500 units
EUR 107
Maximo Taq DNA Polymerase
S103
GeneOn
20x500 units
EUR 328
Taq DNA Polymerase, 5units/㎕
T2200-050
GenDepot
500units
EUR 131
Taq DNA Polymerase, 5units/㎕
T2200-100
GenDepot
2x500 units
EUR 192
Taq DNA Polymerase, 5units/㎕
T2200-200
GenDepot
4x500 units
EUR 313
Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S111
GeneOn
500 units
EUR 53
Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S112
GeneOn
5x500 units
EUR 124
Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S128
GeneOn
20x500 units
EUR 406
Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S129
GeneOn
100x500 units or as bulk
EUR 1785
Taq Plus DNA Polymerase (PCR)
G012
ABM
250 U (50 ul)
EUR 73
Taq Plus DNA Polymerase (PCR)
G040
ABM
1000 U (200 ul)
EUR 106
Taq DNA Polymerase Polyclonal Antibody
7018-100
Biovision
EUR 354
Taq DNA Polymerase Polyclonal Antibody
7018-30T
Biovision
EUR 146
Fast-Taq DNA Polymerase (500U)
9K-001-0036
Bio Basic
500U
EUR 350.54
Taq Plus DNA Polymerase (Recombinant)
abx073522-2500U
Abbexa
2.500 U
EUR 592
Taq Plus DNA Polymerase (Recombinant)
abx073522-250U
Abbexa
250 U
EUR 230
Taq Plus DNA Polymerase (Recombinant)
abx073522-750U
Abbexa
750 U
EUR 328
Recombinant Taq Plus DNA Polymerase
7-03631
CHI Scientific
250U
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Recombinant Taq Plus DNA Polymerase
7-03632
CHI Scientific
750U
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Recombinant Taq Plus DNA Polymerase
7-03633
CHI Scientific
2,500U
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ACTaq? Taq DNA Polymerase, 1000U
E2100-1000U
ACTGene
EUR 217
ACTaq? Taq DNA Polymerase, 250U
E2100-250U
ACTGene
EUR 102
Anti-Taq DNA Polymerase Antibody
48369-100ul
SAB
100ul
EUR 333
Anti-Taq DNA Polymerase Antibody
48369-50ul
SAB
50ul
EUR 239
Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B
Alpha Diagnostics
Custom
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Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U
Alpha Diagnostics
Custom
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Taq DNA Polymerase (Mg2+ plus Buffer)
P101-01
Vazyme
1000 U
EUR 121
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-02
Vazyme
5000 U
EUR 193
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-03
Vazyme
10000 U
EUR 262
Taq DNA Polymerase (Mg2+ free Buffer)
P102-01
Vazyme
1000 U
EUR 121
Taq DNA Polymerase (Mg2+ free Buffer)
P102-02
Vazyme
5000 U
EUR 193
Taq DNA Polymerase (Mg2+ free Buffer)
P102-03
Vazyme
10000 U
EUR 262
Cheetah Hot Start Taq DNA Polymerase
29050
Biotium
500EU
EUR 296
Description: Minimum order quantity: 1 unit of 500EU
amfiSure Hot-Start Taq DNA Polymerase
P0322-010
GenDepot
100 units
EUR 147
amfiSure Hot-Start Taq DNA Polymerase
P0322-025
GenDepot
250 units
EUR 228
ACTaq? Taq Blue DNA Polymerase, 1000U
E2100B-1000U
ACTGene
EUR 227
ACTaq? Taq Blue DNA Polymerase, 250U
E2100B-250U
ACTGene
EUR 105
Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B01
GenDepot
10,000 units
EUR 1157
Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B05
GenDepot
5x10,000 units
EUR 5450
HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-050
GenDepot
500units
EUR 131
HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-100
GenDepot
2x500 units
EUR 192
HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-200
GenDepot
4x500 units
EUR 313
Anti-Taq DNA Polymerase Conjugated Antibody
C48369
SAB
100ul
EUR 397
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N140
GeneOn
500 units
EUR 50
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N142
GeneOn
5x500 units
EUR 120
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N144
GeneOn
20x500 units
EUR 367
Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled
AD-146-F
Alpha Diagnostics
Custom
Ask for price
Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018
Bio Basic
6000U
EUR 1280.62
Taq DNA Polymerase with dNTP Mix (500U)
9K-001-0031
Bio Basic
500U
EUR 292.73
Taq DNA Polymerase with dNTP Mix (3000U)
9K-001-0032
Bio Basic
3000U
EUR 907.39
Taq DNA Polymerase with dNTP Mix (1000U)
9K-001-0034
Bio Basic
1000U
EUR 432.37
Taq DNA Polymerase 2x Premix - 500 Reactions
3249
Intact Genomics
1/EA
EUR 190
Hot Start Taq DNA Polymerase - 1000 units
3293
Intact Genomics
1/EA
EUR 310
amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-010
GenDepot
100 units
EUR 150
amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3
P0330-020
GenDepot
2x100 units
EUR 239
amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-050
GenDepot
5X100 units
EUR 388
amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-250
GenDepot
25X100 units
EUR 1670
amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-500
GenDepot
50X100 units
EUR 2993
Accuris Taq Polymerase
PR1000-1000
BenchMark
1 PC
EUR 172.66
Accuris Taq Polymerase
PR1000-500
BenchMark
1 PC
EUR 132.79
Accuris Taq Polymerase
PR1000-6000
BenchMark
1 PC
EUR 618.47
Accuris Taq Polymerase
PR1000-S
BenchMark
1 PC
EUR 69.79
Tsg PlusDNA Polymerase, 5u/ul
D0088
Bio Basic
200U
EUR 79
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d1
Vazyme
1000 U
EUR 136
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d2
Vazyme
5000 U
EUR 267
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d3
Vazyme
10000 U
EUR 409
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d1
Vazyme
1000 U
EUR 136
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d2
Vazyme
5000 U
EUR 267
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d3
Vazyme
10000 U
EUR 409
Reversible 1-min Hot Start Taq DNA Polymerase
P950
101Bio
-
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Reversible 1-min Hot Start Taq DNA Polymerase
P950L
101Bio
-
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Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-001
GenDepot
1000 units
EUR 165
Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-005
GenDepot
5000 units
EUR 300
Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-010
GenDepot
10,000 units
EUR 583
Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-100
GenDepot
100,000 units
EUR 2885
HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B01
GenDepot
10,000 units
EUR 1157
HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B05
GenDepot
5x10,000 units
EUR 5450
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150
GeneOn
500 units
EUR 102
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152
GeneOn
5x500 units
EUR 394
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154
GeneOn
20x500 units
EUR 1525
T4 DNA Ligase, 5u/ul
B1122
Bio Basic
1KU
EUR 158.75
T4 DNA Ligase, 5u/ul
B1125
Bio Basic
200U
EUR 77.84
EZ Taq Polymerase (HotStart)
9K-005-0020
Bio Basic
500U
EUR 204.86
Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)
9K-001-0003
Bio Basic
500U
EUR 479.35
Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)
9K-001-0004
Bio Basic
2500U
EUR 2054.48
High-Taq DNA Polymerase with 10mM dNTP Mix (250U)
9K-001-0005
Bio Basic
250U
EUR 320.14
High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)
9K-001-0006
Bio Basic
4x250U
EUR 879.98
High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)
9K-001-0020
Bio Basic
5000U
EUR 2399
Taq DNA Polymerase - 5,000 units with separate dNTP Mix
3245d
Intact Genomics
1/EA
EUR 669
amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-025
GenDepot
250 unit
EUR 167
amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-050
GenDepot
2X250 unit
EUR 250
amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-100
GenDepot
4X250 Unit
EUR 421
amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-250
GenDepot
10X250 Unit
EUR 739
amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-050
GenDepot
500 units
EUR 165
amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-102
GenDepot
25x500 units
EUR 1427
amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-500
GenDepot
10X500 units
EUR 739
Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S113
GeneOn
2x100 rcs
EUR 66
Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S114
GeneOn
10x100 rcs
EUR 193
Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S400
GeneOn
200 units
EUR 72
Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S410
GeneOn
1000 units
EUR 193
Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix
3293d
Intact Genomics
1/EA
EUR 357
amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-500
GenDepot
500 units
EUR 219
amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-502
GenDepot
2x500 units
EUR 354
amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-510
GenDepot
10x500 units
EUR 1373
amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-525
GenDepot
25x500 units
EUR 3020
amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-550
GenDepot
50x500 units
EUR 5383
Fast DNA Ligation Kit (5u/ul, T4)
BS511
Bio Basic
10prep
EUR 70.88
Fast DNA Ligation Kit (5u/ul, T4)
BS512
Bio Basic
50prep
EUR 132.65
T4 DNA Ligase with PEG, 5u/ul
B1442
Bio Basic
1KU
EUR 163.1
T4 DNA Ligase with PEG, 5u/ul
B1445
Bio Basic
200U
EUR 80.45
PFU DNA Polymerase
9003-2500
Biovision
EUR 512
PFU DNA Polymerase
9003-500
Biovision
EUR 180
Laq? DNA Polymerase
9004-2500
Biovision
EUR 610
Laq? DNA Polymerase
9004-500
Biovision
EUR 218
Kodaq DNA Polymerase
G498
ABM
250 U (50 ul)
EUR 87
Kodaq DNA Polymerase
G499
ABM
1000 U (200 ul)
EUR 158
AceTaq DNA Polymerase
P401-d1
Vazyme
250 U
EUR 136
AceTaq DNA Polymerase
P401-d2
Vazyme
1000 U
EUR 227
AceTaq DNA Polymerase
P401-d3
Vazyme
3000 U
EUR 447
Ready? DNA Polymerase
M1146-1000
Biovision
EUR 332
Ready? DNA Polymerase
M1146-10000
Biovision
EUR 1066
Ready? DNA Polymerase
M1146-5000
Biovision
EUR 800
Breeze? DNA Polymerase
M1148-1000
Biovision
EUR 1039
Breeze? DNA Polymerase
M1148-250
Biovision
EUR 387
Distant? DNA Polymerase
M1150-1000
Biovision
EUR 686
Distant? DNA Polymerase
M1150-250
Biovision
EUR 278
Advance? DNA Polymerase
M1151-1000
Biovision
EUR 746
Advance? DNA Polymerase
M1151-250
Biovision
EUR 311
Outstretched? DNA Polymerase
M1152-1000
Biovision
EUR 800
Outstretched? DNA Polymerase
M1152-250
Biovision
EUR 332
T4 DNA Polymerase
M1211-100
Biovision
EUR 321
Phi29 DNA Polymerase
M1239-1000
Biovision
EUR 311
T4 DNA Polymerase
N101-01
Vazyme
2000 U
EUR 232
DNA polymerase ? (Rat)
10-101
Sceti
20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.
DNA polymerase ? (Rat)
10-102
Sceti
100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.
BIOTAQ DNA Polymerase
BIO-21040
Bioline
500 Units
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IMMOLASE DNA Polymerase
BIO-21046
Bioline
250 Units
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IMMOLASE DNA Polymerase
BIO-21047
Bioline
500 Units
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IMMOLASE DNA Polymerase
BIO-21048
Bioline
5000 Units
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The introduction of mutual compensation costs to fluorophores or other functional groups dNTP conjugation can be considered as a basis for the preparation of modified nucleoside PCR-compatible triphosphates.Using Sso7d from Sulfolobus solfataricus as DNA binding protein fusion Taq DNA polymerase at the amino end, we reported hyper-expression and purification methodology novel Sso7d-Taq polymerase (S-Taq) using a two-phase system of water extraction followed by Ni-affinity chromatography.