Posted in Antibodies, Assay Kits, Biology Cells, cDNA, Clia Kits, Culture Cells, Devices, DNA, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, Recombinant Proteins, Ria Kits, RNA, Test Kits, Vector & Virus, Western Blot

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

DNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. They are used in various fields of science, especially as an important component for the in vitro synthesis of DNA, known as PCR. modern diagnostics, molecular biology and genetic engineering of DNA polymerase needs which demonstrate improved performance. This study aims to get NeqSSB-TaqS new DNA polymerase fusion of domain Stoffel Taq DNA and single-stranded DNA binding protein from Nanoarchaeum equitans as to significantly improve the properties of the DNA polymerase.

Stoffel Taq DNA coding sequence specific DNA polymerase and DNA-binding proteins Nanoarchaeum equitans (NeqSSB-like protein) fused. A new recombinant genes obtained were cloned into pET-30 Ek / LIC vector and introduced into E. coli for expression. Purified recombinant enzymes and enzymatic properties including the activity of DNA polymerase, PCR amplification level, thermostability, processivity and resistance to inhibitors, were tested. The results of the target protein around 18 mg / l after 24 hours of IPTG induction.

The specific activity of the polymerase is 2200 U / mg. Recombinant NeqSSB-TaqS exhibited a higher level extensions (1000 bp template 20 s), processivity (19 nt), thermostability (half-life 35 minutes at 95 ° C) and a higher tolerance for PCR inhibitors (0.3 to 1.25 % of whole blood, 0.84 to 13.5 ug heparin lactoferrin and 4.7 to 150 ng) of Taq DNA polymerase Stoffel. In addition, our research shows that NeqSSB-TaqS DNA polymerase has a high degree of flexibility in relation to the Mg 2+ ions (from 1 to 5 mM) and KCl or (NH4) 2SO4 salt (more than 60 mM and 40 mM, respectively ). NeqSSB-TaqS using DNA polymerase Taq DNA polymerase can not be a better choice in many applications of PCR.

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.
Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

dUTPs zwitterionic conjugated with Cy3 or Cy5 analog Fluorophore effective substrate for DNA amplification and labeling by Taq polymerase.

To develop the structural modifications dNTP compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dyes of different analog responsible and charge distribution throughout the fluorophore. Cy3- derivatives and commercial dUTP and Cy5-dUTP studied in Taq polymerase dependent polymerase chain reaction (PCR) and the primer extension reaction using a model template that contains one, two and three adjacent nucleotides adenine.

The relative amount of DNA is amplified and kinetic parameters Km and Vmax characterize grief labeled incorporation have been estimated using fluorescence measurements and analyzed. The dUTPs label with analog zwitterionic electroneutral of Cy3 or Cy5 fluorophores Taq polymerase is used by approximately one order of magnitude more effective than dUTPs analog labeled with a negatively charged of Cy3 or Cy5. Nucleotidyl transferase activity of Taq polymerase were also observed and resulted in the addition dumps electroneutral labeled with fluorophores or positively charged to the 3 ‘end of the DNA.

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ500 500U Ask for price

3G HotStart Taq DNA Polymerase (5u/ul)
3GHST81 5x500U
EUR 746.87

Taq DNA Polymerase (High Purity), 5u/ul
HTD0078 5X200U
EUR 108

Taq DNA Polymerase
9001-2500
EUR 512

Taq DNA Polymerase
9001-500
EUR 191

Taq DNA Polymerase
L7051001 1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase
L7051200 200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase
BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

T4 DNA Polymerase, 5u/ul
BEP0061 100U
EUR 128.31

Pfu DNA Polymerase, 5u/ul
B0093(D0090P) 500U
EUR 137

Tsg DNA Polymerase, 5u/ul
D0081 5X200U
EUR 93.5

Taq DNA Polymerase (PCR)
G008 5000 U (1.0 ml)
EUR 205

Taq DNA Polymerase (PCR)
G009 1000 U (200 ul)
EUR 87

Taq DNA Polymerase (PCR)
G126 10000 U (2 x 1.0 ml)
EUR 309

Champagne Taq DNA Polymerase
P122-d1 500 U (2.5 U/μl)
EUR 177

Champagne Taq DNA Polymerase
P122-d2 500 U (5 U/μl)
EUR 177

Champagne Taq DNA Polymerase
P122-d3 500 U (10 U/μl)
EUR 177

Taq Plus DNA Polymerase
P201-01 250 U
EUR 123

Taq Plus DNA Polymerase
P201-02 1000 U
EUR 178

Taq Plus DNA Polymerase
P201-03 3000 U
EUR 319

Hotstart Taq DNA Polymerase
L7052001 1000 units
EUR 568
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase
L7052250 250 units
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase (500U)
9K-001-0001 500U
EUR 174.41

Taq DNA Polymerase (3000U)
9K-001-0002 3000U
EUR 485

Taq DNA Polymerase (6000U)
9K-001-0033 6000U
EUR 1028.75

Taq DNA Polymerase (1000U)
9K-001-0035 1000U
EUR 348.85

Taq DNA Polymerase (Recombinant)
abx073521-10000U 10000 U
EUR 592

Taq DNA Polymerase (Recombinant)
abx073521-1000U 1000 U
EUR 230

Taq DNA Polymerase (Recombinant)
abx073521-3000U 3000 U
EUR 328

Recombinant Taq DNA Polymerase
7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase
7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase
7-03636 10,000U Ask for price

Taq DNA polymerase (+dNTPs)
02-001 200U
EUR 153
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)
02-001-5 5×200U
EUR 257
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)
02-011 200U
EUR 140
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)
02-011-5 5×200U
EUR 218
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase
S101 500 units
EUR 46

Maximo Taq DNA Polymerase
S102 5x500 units
EUR 107

Maximo Taq DNA Polymerase
S103 20x500 units
EUR 328

Taq DNA Polymerase, 5units/㎕
T2200-050 500units
EUR 131

Taq DNA Polymerase, 5units/㎕
T2200-100 2x500 units
EUR 192

Taq DNA Polymerase, 5units/㎕
T2200-200 4x500 units
EUR 313

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S111 500 units
EUR 53

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S112 5x500 units
EUR 124

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S128 20x500 units
EUR 406

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S129 100x500 units or as bulk
EUR 1785

Taq Plus DNA Polymerase (PCR)
G012 250 U (50 ul)
EUR 73

Taq Plus DNA Polymerase (PCR)
G040 1000 U (200 ul)
EUR 106

Taq DNA Polymerase Polyclonal Antibody
7018-100
EUR 354

Taq DNA Polymerase Polyclonal Antibody
7018-30T
EUR 146

Fast-Taq DNA Polymerase (500U)
9K-001-0036 500U
EUR 350.54

Taq Plus DNA Polymerase (Recombinant)
abx073522-2500U 2.500 U
EUR 592

Taq Plus DNA Polymerase (Recombinant)
abx073522-250U 250 U
EUR 230

Taq Plus DNA Polymerase (Recombinant)
abx073522-750U 750 U
EUR 328

Taq DNA Polymerase - 5,000 units
3245 1/EA
EUR 517

Recombinant Taq Plus DNA Polymerase
7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase
7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase
7-03633 2,500U Ask for price

YEA taq DNA Polymerase wiodNTP
FYT011-500U 500U Ask for price

ACTaq? Taq DNA Polymerase, 1000U
E2100-1000U
EUR 217

ACTaq? Taq DNA Polymerase, 250U
E2100-250U
EUR 102

Anti-Taq DNA Polymerase Antibody
48369-100ul 100ul
EUR 333

Anti-Taq DNA Polymerase Antibody
48369-50ul 50ul
EUR 239

Recombinant (E.Coli) Taq DNA Polymerase
RP-394 1000 IU
EUR 164

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U Custom Ask for price

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-03 10000 U
EUR 262

Taq DNA Polymerase (Mg2+ free Buffer)
P102-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ free Buffer)
P102-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ free Buffer)
P102-03 10000 U
EUR 262

Cheetah Hot Start Taq DNA Polymerase
29050 500EU
EUR 296
Description: Minimum order quantity: 1 unit of 500EU

YEA taq DNA Polymerase wi dNTP
FYT001-500U 500U Ask for price

amfiSure Hot-Start Taq DNA Polymerase
P0322-010 100 units
EUR 147

amfiSure Hot-Start Taq DNA Polymerase
P0322-025 250 units
EUR 228

ACTaq? Taq Blue DNA Polymerase, 1000U
E2100B-1000U
EUR 227

ACTaq? Taq Blue DNA Polymerase, 250U
E2100B-250U
EUR 105

Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B01 10,000 units
EUR 1157

Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B05 5x10,000 units
EUR 5450

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-050 500units
EUR 131

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-100 2x500 units
EUR 192

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-200 4x500 units
EUR 313

Anti-Taq DNA Polymerase Conjugated Antibody
C48369 100ul
EUR 397

Recombinant (E.Coli) Taq Plus DNA Polymerase
RP-395 250 U
EUR 164

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N140 500 units
EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N142 5x500 units
EUR 120

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N144 20x500 units
EUR 367

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled
AD-146-F Custom Ask for price

Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018 6000U
EUR 1280.62

Taq DNA Polymerase with dNTP Mix (500U)
9K-001-0031 500U
EUR 292.73

Taq DNA Polymerase with dNTP Mix (3000U)
9K-001-0032 3000U
EUR 907.39

Taq DNA Polymerase with dNTP Mix (1000U)
9K-001-0034 1000U
EUR 432.37

Taq DNA Polymerase 2x Premix - 500 Reactions
3249 1/EA
EUR 190

Hot Start Taq DNA Polymerase - 1000 units
3293 1/EA
EUR 310

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-010 100 units
EUR 150

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3
P0330-020 2x100 units
EUR 239

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-050 5X100 units
EUR 388

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-250 25X100 units
EUR 1670

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-500 50X100 units
EUR 2993

Accuris Taq Polymerase
PR1000-1000 1 PC
EUR 172.66

Accuris Taq Polymerase
PR1000-500 1 PC
EUR 132.79

Accuris Taq Polymerase
PR1000-6000 1 PC
EUR 618.47

Accuris Taq Polymerase
PR1000-S 1 PC
EUR 69.79

Tsg PlusDNA Polymerase, 5u/ul
D0088 200U
EUR 79

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d3 10000 U
EUR 409

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d3 10000 U
EUR 409

Reversible 1-min Hot Start Taq DNA Polymerase
P950 - Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase
P950L - Ask for price

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-001 1000 units
EUR 165

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-005 5000 units
EUR 300

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-010 10,000 units
EUR 583

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-100 100,000 units
EUR 2885

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B01 10,000 units
EUR 1157

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B05 5x10,000 units
EUR 5450

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152 5x500 units
EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154 20x500 units
EUR 1525

T4 DNA Ligase, 5u/ul
B1122 1KU
EUR 158.75

T4 DNA Ligase, 5u/ul
B1125 200U
EUR 77.84

EZ Taq Polymerase (HotStart)
9K-005-0020 500U
EUR 204.86

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)
9K-001-0003 500U
EUR 479.35

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)
9K-001-0004 2500U
EUR 2054.48

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)
9K-001-0005 250U
EUR 320.14

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)
9K-001-0006 4x250U
EUR 879.98

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)
9K-001-0020 5000U
EUR 2399

Taq DNA Polymerase - 5,000 units with separate dNTP Mix
3245d 1/EA
EUR 669

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-025 250 unit
EUR 167

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-050 2X250 unit
EUR 250

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-100 4X250 Unit
EUR 421

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-250 10X250 Unit
EUR 739

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-050 500 units
EUR 165

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-102 25x500 units
EUR 1427

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-500 10X500 units
EUR 739

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S113 2x100 rcs
EUR 66

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S114 10x100 rcs
EUR 193

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S400 200 units
EUR 72

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S410 1000 units
EUR 193

DNA Polymerase
ABD3082 100 ug
EUR 438

DNA Polymerase
ABF5235 100 ug
EUR 438

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix
3293d 1/EA
EUR 357

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-500 500 units
EUR 219

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-502 2x500 units
EUR 354

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-510 10x500 units
EUR 1373

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-525 25x500 units
EUR 3020

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-550 50x500 units
EUR 5383

Fast DNA Ligation Kit (5u/ul, T4)
BS511 10prep
EUR 70.88

Fast DNA Ligation Kit (5u/ul, T4)
BS512 50prep
EUR 132.65

T4 DNA Ligase with PEG, 5u/ul
B1442 1KU
EUR 163.1

T4 DNA Ligase with PEG, 5u/ul
B1445 200U
EUR 80.45

Taq DNA Ligase - 10,000 units
3219 1/EA
EUR 253

PFU DNA Polymerase
9003-2500
EUR 512

PFU DNA Polymerase
9003-500
EUR 180

Laq? DNA Polymerase
9004-2500
EUR 610

Laq? DNA Polymerase
9004-500
EUR 218

Kodaq DNA Polymerase
G498 250 U (50 ul)
EUR 87

Kodaq DNA Polymerase
G499 1000 U (200 ul)
EUR 158

AceTaq DNA Polymerase
P401-d1 250 U
EUR 136

AceTaq DNA Polymerase
P401-d2 1000 U
EUR 227

AceTaq DNA Polymerase
P401-d3 3000 U
EUR 447

Ready? DNA Polymerase
M1146-1000
EUR 332

Ready? DNA Polymerase
M1146-10000
EUR 1066

Ready? DNA Polymerase
M1146-5000
EUR 800

Breeze? DNA Polymerase
M1148-1000
EUR 1039

Breeze? DNA Polymerase
M1148-250
EUR 387

Distant? DNA Polymerase
M1150-1000
EUR 686

Distant? DNA Polymerase
M1150-250
EUR 278

Advance? DNA Polymerase
M1151-1000
EUR 746

Advance? DNA Polymerase
M1151-250
EUR 311

Outstretched? DNA Polymerase
M1152-1000
EUR 800

Outstretched? DNA Polymerase
M1152-250
EUR 332

T4 DNA Polymerase
M1211-100
EUR 321

Phi29 DNA Polymerase
M1239-1000
EUR 311

T4 DNA Polymerase
N101-01 2000 U
EUR 232

DNA polymerase ? (Rat)
10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)
10-102 100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

BIOTAQ DNA Polymerase
BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase
BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase
BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase
BIO-21048 5000 Units Ask for price

The introduction of mutual compensation costs to fluorophores or other functional groups dNTP conjugation can be considered as a basis for the preparation of modified nucleoside PCR-compatible triphosphates.Using Sso7d from Sulfolobus solfataricus as DNA binding protein fusion Taq DNA polymerase at the amino end, we reported hyper-expression and purification methodology novel Sso7d-Taq polymerase (S-Taq) using a two-phase system of water extraction followed by Ni-affinity chromatography.

Jordan

Leave A Comment