Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

DNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. They are used in various fields of science, especially as an important component for the in vitro synthesis of DNA, known as PCR. modern diagnostics, molecular biology and genetic engineering of DNA polymerase needs which demonstrate improved performance. This study aims to get NeqSSB-TaqS new DNA polymerase fusion of domain Stoffel Taq DNA and single-stranded DNA binding protein from Nanoarchaeum equitans as to significantly improve the properties of the DNA polymerase.

Stoffel Taq DNA coding sequence specific DNA polymerase and DNA-binding proteins Nanoarchaeum equitans (NeqSSB-like protein) fused. A new recombinant genes obtained were cloned into pET-30 Ek / LIC vector and introduced into E. coli for expression. Purified recombinant enzymes and enzymatic properties including the activity of DNA polymerase, PCR amplification level, thermostability, processivity and resistance to inhibitors, were tested. The results of the target protein around 18 mg / l after 24 hours of IPTG induction.

The specific activity of the polymerase is 2200 U / mg. Recombinant NeqSSB-TaqS exhibited a higher level extensions (1000 bp template 20 s), processivity (19 nt), thermostability (half-life 35 minutes at 95 ° C) and a higher tolerance for PCR inhibitors (0.3 to 1.25 % of whole blood, 0.84 to 13.5 ug heparin lactoferrin and 4.7 to 150 ng) of Taq DNA polymerase Stoffel. In addition, our research shows that NeqSSB-TaqS DNA polymerase has a high degree of flexibility in relation to the Mg 2+ ions (from 1 to 5 mM) and KCl or (NH4) 2SO4 salt (more than 60 mM and 40 mM, respectively ). NeqSSB-TaqS using DNA polymerase Taq DNA polymerase can not be a better choice in many applications of PCR.

 Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.
Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

dUTPs zwitterionic conjugated with Cy3 or Cy5 analog Fluorophore effective substrate for DNA amplification and labeling by Taq polymerase.

To develop the structural modifications dNTP compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dyes of different analog responsible and charge distribution throughout the fluorophore. Cy3- derivatives and commercial dUTP and Cy5-dUTP studied in Taq polymerase dependent polymerase chain reaction (PCR) and the primer extension reaction using a model template that contains one, two and three adjacent nucleotides adenine.

The relative amount of DNA is amplified and kinetic parameters Km and Vmax characterize grief labeled incorporation have been estimated using fluorescence measurements and analyzed. The dUTPs label with analog zwitterionic electroneutral of Cy3 or Cy5 fluorophores Taq polymerase is used by approximately one order of magnitude more effective than dUTPs analog labeled with a negatively charged of Cy3 or Cy5. Nucleotidyl transferase activity of Taq polymerase were also observed and resulted in the addition dumps electroneutral labeled with fluorophores or positively charged to the 3 ‘end of the DNA.

Taq DNA Polymerase, 5 units/µl

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Taq DNA Polymerase, 5 units/µl

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Taq DNA Polymerase, 5 units/µl

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Taq DNA Polymerase, 5 units/µl

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Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 108
  • Product category: PCR Related/Enzymes/DNA Polymerases

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EUR 746.87
  • Product category: PCR Related/Enzymes/DNA Polymerases

Taq DNA Polymerase

BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

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EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500
EUR 512

Taq DNA Polymerase

9001-500
EUR 191

Taq DNA Polymerase

L7051001 1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pfu DNA Polymerase, 5u/ul

B0093(D0090P) 500U
EUR 137
  • Product category: PCR Related/Enzymes/DNA Polymerases

T4 DNA Polymerase, 5u/ul

BEP0061 100U
EUR 128.31
  • Product category: PCR Related/Enzymes/DNA Polymerases

Tsg DNA Polymerase, 5u/ul

D0081 5X200U
EUR 93.5
  • Product category: PCR Related/Enzymes/DNA Polymerases

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 205

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 87

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 309

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 592
  • Shipped within 5-10 working days.

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 230
  • Shipped within 5-10 working days.

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 328
  • Shipped within 5-10 working days.

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 153
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 257
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 140
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 218
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 177

Taq Plus DNA Polymerase

P201-01 250 U
EUR 123

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 178

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 319

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 568
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Maximo Taq DNA Polymerase

S101 500 units
EUR 46

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 107

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 328

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 131

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 192

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 313

Taq DNA Polymerase (500U)

9K-001-0001 500U
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  • Product category: PCR Related/Enzymes/DNA Polymerases

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Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

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Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

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Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

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Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S129 100x500 units or as bulk
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ACTaq? Taq DNA Polymerase, 1000U

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E2100-250U
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YEA taq DNA Polymerase wiodNTP

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Taq Plus DNA Polymerase (PCR)

G012 250 U (50 ul)
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Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
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Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 592
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Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
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Taq Plus DNA Polymerase (Recombinant)

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EUR 328
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Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 333

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 239

Taq DNA Polymerase - 5,000 units

3245 1/EA
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Taq DNA Polymerase Polyclonal Antibody

7018-100
EUR 354

Taq DNA Polymerase Polyclonal Antibody

7018-30T
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Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 164

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 350.54
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Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

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Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 397

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U
EUR 227

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U
EUR 105

YEA taq DNA Polymerase wi dNTP

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Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 296
Description: Minimum order quantity: 1 unit of 500EU

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 262

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 262

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 147

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 228

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 164

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1157

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 5450

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 131

HiQTM Taq Red DNA Polymerase, 1unit/㎕

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EUR 313

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 120

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 367

Hot Start Taq DNA Polymerase - 1000 units

3293 1/EA
EUR 310

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 190

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-010 100 units
EUR 150

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3

P0330-020 2x100 units
EUR 239

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-050 5X100 units
EUR 388

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-250 25X100 units
EUR 1670

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-500 50X100 units
EUR 2993

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1280.62
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 292.73
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 907.39
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 432.37
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Accuris Taq Polymerase

PR1000-1000 1 PC
EUR 172.66
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Polymerase

PR1000-500 1 PC
EUR 132.79
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Polymerase

PR1000-6000 1 PC
EUR 618.47
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Polymerase

PR1000-S 1 PC
EUR 69.79
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 409

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 409

Reversible 1-min Hot Start Taq DNA Polymerase

P950 NULL
EUR 0

Reversible 1-min Hot Start Taq DNA Polymerase

P950L NULL
EUR 0

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 165

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 300

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 583

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-100 100,000 units
EUR 2885

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B01 10,000 units
EUR 1157

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B05 5x10,000 units
EUR 5450

Tsg PlusDNA Polymerase, 5u/ul

D0088 200U
EUR 79
  • Product category: PCR Related/Enzymes/DNA Polymerases

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1525

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 669

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-025 250 unit
EUR 167

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-050 2X250 unit
EUR 250

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-100 4X250 Unit
EUR 421

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-250 10X250 Unit
EUR 739

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 479.35
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

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9K-001-0004 2500U
EUR 2054.48
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 320.14
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 879.98
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2399
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

EZ Taq Polymerase (HotStart)

9K-005-0020 500U
EUR 204.86
  • Product category: PCR Related/Enzymes/DNA Polymerases

T4 DNA Ligase, 5u/ul

B1122 1KU
EUR 158.75
  • Product category: Proteins/Enzymes/DNA Ligases

T4 DNA Ligase, 5u/ul

B1125 200U
EUR 77.84
  • Product category: Proteins/Enzymes/DNA Ligases

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-050 500 units
EUR 165

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-102 25x500 units
EUR 1427

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-500 10X500 units
EUR 739

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S113 2x100 rcs
EUR 66

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S114 10x100 rcs
EUR 193

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 72

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 193

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 357

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-500 500 units
EUR 219

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-502 2x500 units
EUR 354

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-510 10x500 units
EUR 1373

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-525 25x500 units
EUR 3020

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-550 50x500 units
EUR 5383

Taq DNA Ligase - 10,000 units

3219 1/EA
EUR 253

T4 DNA Ligase with PEG, 5u/ul

B1442 1KU
EUR 163.1
  • Product category: Proteins/Enzymes/DNA Ligases

T4 DNA Ligase with PEG, 5u/ul

B1445 200U
EUR 80.45
  • Product category: Proteins/Enzymes/DNA Ligases

Fast DNA Ligation Kit (5u/ul, T4)

BS511 10prep
EUR 70.88
  • Product category: Molecular Biology Kits/Blunting/Ligation

Fast DNA Ligation Kit (5u/ul, T4)

BS512 50prep
EUR 132.65
  • Product category: Molecular Biology Kits/Blunting/Ligation

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

HotTaq DNA Polymerase

BA00203 500U
EUR 145
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 239
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 522
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 123
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

The introduction of mutual compensation costs to fluorophores or other functional groups dNTP conjugation can be considered as a basis for the preparation of modified nucleoside PCR-compatible triphosphates.Using Sso7d from Sulfolobus solfataricus as DNA binding protein fusion Taq DNA polymerase at the amino end, we reported hyper-expression and purification methodology novel Sso7d-Taq polymerase (S-Taq) using a two-phase system of water extraction followed by Ni-affinity chromatography.

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