Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

DNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. They are used in various fields of science, especially as an important component for the in vitro synthesis of DNA, known as PCR. modern diagnostics, molecular biology and genetic engineering of DNA polymerase needs which demonstrate improved performance. This study aims to get NeqSSB-TaqS new DNA polymerase fusion of domain Stoffel Taq DNA and single-stranded DNA binding protein from Nanoarchaeum equitans as to significantly improve the properties of the DNA polymerase.

Stoffel Taq DNA coding sequence specific DNA polymerase and DNA-binding proteins Nanoarchaeum equitans (NeqSSB-like protein) fused. A new recombinant genes obtained were cloned into pET-30 Ek / LIC vector and introduced into E. coli for expression. Purified recombinant enzymes and enzymatic properties including the activity of DNA polymerase, PCR amplification level, thermostability, processivity and resistance to inhibitors, were tested. The results of the target protein around 18 mg / l after 24 hours of IPTG induction.

The specific activity of the polymerase is 2200 U / mg. Recombinant NeqSSB-TaqS exhibited a higher level extensions (1000 bp template 20 s), processivity (19 nt), thermostability (half-life 35 minutes at 95 ° C) and a higher tolerance for PCR inhibitors (0.3 to 1.25 % of whole blood, 0.84 to 13.5 ug heparin lactoferrin and 4.7 to 150 ng) of Taq DNA polymerase Stoffel. In addition, our research shows that NeqSSB-TaqS DNA polymerase has a high degree of flexibility in relation to the Mg 2+ ions (from 1 to 5 mM) and KCl or (NH4) 2SO4 salt (more than 60 mM and 40 mM, respectively ). NeqSSB-TaqS using DNA polymerase Taq DNA polymerase can not be a better choice in many applications of PCR.

 Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.
Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

dUTPs zwitterionic conjugated with Cy3 or Cy5 analog Fluorophore effective substrate for DNA amplification and labeling by Taq polymerase.

To develop the structural modifications dNTP compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dyes of different analog responsible and charge distribution throughout the fluorophore. Cy3- derivatives and commercial dUTP and Cy5-dUTP studied in Taq polymerase dependent polymerase chain reaction (PCR) and the primer extension reaction using a model template that contains one, two and three adjacent nucleotides adenine.

The relative amount of DNA is amplified and kinetic parameters Km and Vmax characterize grief labeled incorporation have been estimated using fluorescence measurements and analyzed. The dUTPs label with analog zwitterionic electroneutral of Cy3 or Cy5 fluorophores Taq polymerase is used by approximately one order of magnitude more effective than dUTPs analog labeled with a negatively charged of Cy3 or Cy5. Nucleotidyl transferase activity of Taq polymerase were also observed and resulted in the addition dumps electroneutral labeled with fluorophores or positively charged to the 3 ‘end of the DNA.

Taq DNA Polymerase, 5 units/µl

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Taq DNA polymerase

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

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EUR 229.2

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

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EUR 234
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

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EUR 104.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Tsg DNA Polymerase, 5u/ul

D0081 5X200U
EUR 112.2

T4 DNA Polymerase, 5u/ul

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EUR 153.97

Pfu DNA Polymerase, 5u/ul

B0093(D0090P) 500U
EUR 164.4

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

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Taq DNA polymerase (+dNTPs)

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EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

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Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

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EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

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EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

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EUR 393.6

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
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9K-001-0033 6000U
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9K-001-0035 1000U
EUR 418.62

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

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EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 681.6
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 234
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

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G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

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EUR 104.4

Taq DNA Polymerase (PCR)

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Maximo Taq DNA Polymerase

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Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

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EUR 487.2

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Anti-Taq DNA Polymerase Antibody

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EUR 399.6

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Taq DNA Polymerase - 5,000 units

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Recombinant Taq Plus DNA Polymerase

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EUR 393.6

Fast-Taq DNA Polymerase (500U)

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G040 1000 U (200 ul)
EUR 127.2

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GR108007 200 tests
EUR 276

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RP-394 1000 IU
EUR 196.8

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Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 476.4

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 355.2
Description: Minimum order quantity: 1 unit of 500EU

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 176.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 273.6

Taq DNA Polymerase (Mg2+ plus Buffer)

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EUR 145.2

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EUR 231.6

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Taq DNA Polymerase 2x Premix - 500 Reactions

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EUR 1536.74

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EUR 1088.87

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HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

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Tsg PlusDNA Polymerase, 5u/ul

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Accuris Taq Polymerase

PR1000-1000 1 PC
EUR 207.19

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EUR 159.35

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EUR 742.16

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EUR 83.75

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

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EUR 163.2

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EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 490.8

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

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EUR 163.2

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 320.4

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EUR 490.8

Reversible 1-min Hot Start Taq DNA Polymerase

P950 - Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase

P950L - Ask for price

Genorise® CLT Taq DNA Polymerase (1000 U)

GR108003 1000 U
EUR 238.8

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 198

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 360

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 699.6

Taq DNA Polymerase, Ultra Pure, 5 units/ul

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EUR 3462

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

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EUR 1388.4

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EUR 6540

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 122.4

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

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EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

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EUR 1830

T4 DNA Ligase, 5u/ul

B1122 1KU
EUR 190.5

T4 DNA Ligase, 5u/ul

B1125 200U
EUR 93.41

EZ Taq Polymerase (HotStart)

9K-005-0020 500U
EUR 245.83

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 575.22

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2465.38

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 384.17

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

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EUR 1055.98

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

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EUR 2878.8

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

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amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

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amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

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amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

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amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

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EUR 198

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

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EUR 1712.4

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

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EUR 886.8

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S113 2x100 rcs
EUR 79.2

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S114 10x100 rcs
EUR 231.6

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

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DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 428.4

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-500 500 units
EUR 262.8

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

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EUR 1647.6

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P7002-525 25x500 units
EUR 3624

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EUR 6459.6

Fast DNA Ligation Kit (5u/ul, T4)

BS511 10prep
EUR 85.06

Fast DNA Ligation Kit (5u/ul, T4)

BS512 50prep
EUR 159.18

T4 DNA Ligase with PEG, 5u/ul

B1442 1KU
EUR 195.72

T4 DNA Ligase with PEG, 5u/ul

B1445 200U
EUR 96.54

Taq DNA Ligase - 10,000 units

3219 1/EA
EUR 303.6

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

PFU DNA Polymerase

9003-2500
EUR 614.4

PFU DNA Polymerase

9003-500
EUR 216

Laq? DNA Polymerase

9004-2500
EUR 732

Laq? DNA Polymerase

9004-500
EUR 261.6

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
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BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

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BIOTAQ DNA Polymerase

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MangoTaq DNA Polymerase

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BIO-21082 2000 Units Ask for price

The introduction of mutual compensation costs to fluorophores or other functional groups dNTP conjugation can be considered as a basis for the preparation of modified nucleoside PCR-compatible triphosphates.Using Sso7d from Sulfolobus solfataricus as DNA binding protein fusion Taq DNA polymerase at the amino end, we reported hyper-expression and purification methodology novel Sso7d-Taq polymerase (S-Taq) using a two-phase system of water extraction followed by Ni-affinity chromatography.

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