Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering.

Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering.

DNA polymerase is widely used for manipulating DNA in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. thermostable DNA polymerase is very useful and can be quite worthwhile after the development of PCR technology. A DNA polymerases from Thermus aquaticus (Taq polymerase) is the most well-known DNA polymerase as PCR enzyme, and has been widely used around the world. In this study, the gene fragment of family A DNA polymerase was amplified by PCR from the DNA of microorganisms in environmental soil samples, using a set of primers for two conserved regions.

The corresponding region of the pol gene for Taq polymerase gene fragment was replaced with reinforced, and a variety of chimeric DNA polymerases prepared. Based on these properties chimeric enzymes and their sequence, two residues, E742 and A743, in the Taq polymerase was found to be important for the ability of elongation. Taq polymerase with mutations in the 742 and 743 actually showed a higher affinity DNA and primer extension capabilities faster. These factors also affect the performance of PCR on DNA polymerase, and the PCR yield improvement was observed with Taq polymerase mutants. Fast-cycling PCR formulations, protocols, and instruments have been developed to address the need to improve throughput and shorter turn-around times for PCR-based tests.

Although the run time can be cut by up to 50% shorter cycle times have been correlated with a lower detection sensitivity and increased variability. To solve this problem, we apply the self compartmentalized Replication (CSR) to develop faster-cycling mutant Taq DNA polymerase. After five rounds of selection using a shorter PCR extension times, individual mutations identified in the fastest-cycling clones randomly combined using ligation-based multi-site mutagenesis.

The best performing combination mutants showed 35- 90-fold higher affinity (lower Kd) for excellent template and (2-fold) increase in the rate of extension moderate compared to wild-type Taq. Further characterization revealed that mutations selected CSR provides increased resistance to inhibitors, and in particular, enable direct amplification of up to 65% whole blood. We discuss the contribution of individual mutations for fast-cycling and blood-resistant phenotype.

 Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering.
Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering.

The increase in the gap repair cloning in yeast: vector treatment gap with Taq DNA polymerase Avoid self-ligation of the vector.

Gap repair a fast and efficient method for the assembly of recombinant DNA molecules in Saccharomyces cerevisiae. This method produces a circular DNA molecule by homologous recombination between two or more linear DNA fragments, one of which is usually the vector carrying the replicative sequence and a selective marker.

This technique avoids laborious and expensive in vitro purification and DNA ligation. DNA repair machinery can also close and ligate linear vector by a mechanism other than homologous recombination, resulting in an empty vector. The frequency of adverse events can be reduced by removing the 5′-phosphate group using a phosphatase, which is the standard method used for in vitro ligation.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Taq DNA Polymerase

G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA Polymerase

L7051001 1000 unit
EUR 167

Taq DNA Polymerase

L7051200 200 unit
EUR 43

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

Taq HS DNA Polymerase

P132-d1 500 U
EUR 47.85

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 89.18

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 406

TM Taq DNA Polymerase

TBS4010 1000 U
EUR 60

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

Taq DNA Polymerase Mix

abx461043-1096tests 10 × 96 tests Ask for price

Taq DNA Polymerase Mix

abx461043-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase Mix

abx461043-96tests 96 tests
EUR 337.5

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase

S101 500 units
EUR 55.2

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 128.4

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 393.6

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 595

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 167

Taq DNA Polymerase -1000 units

3243 1000units
EUR 115
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 449
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Taq DNA Polymerase (Recombinant)

22060707-1 1,000 Unit(s)
EUR 137.27

Taq DNA Polymerase (Recombinant)

22060707-2 1500 Unit(s)
EUR 182.1

Taq DNA Polymerase (Recombinant)

22060707-3 5000 Unit(s)
EUR 465.77

Taq DNA Polymerase (Recombinant)

22060707-4 10 KU
EUR 840.96

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 607.62

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA Polymerase Recombinant

rAP-1062 Inquiry Ask for price

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

Taq DNA Polymerase (5U/uL)

A1003-500U 500U
EUR 26.4
Description: Biotechnology

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

Taq Plus DNA Polymerase (Taq Plus DNA) Protein

abx073522-100tests 100 tests
EUR 225

Taq DNA Polymerase (5U/µl), 2000U

PL1204 each Ask for price

Hot start Taq DNA Polymerase

EQ006 100μl/500U
EUR 200

Hot Start Taq DNA Polymerase

abx460010-1096tests 10 × 96 tests Ask for price

Hot Start Taq DNA Polymerase

abx460010-596tests 5 × 96 tests Ask for price

Hot Start Taq DNA Polymerase

abx460010-96tests 96 tests
EUR 262.5

Taq DNA Polymerase (5U/µl), 500U

PL1202 each Ask for price

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

Taq DNA Polymerase (TaqDNA) Protein

abx073521-100tests 100 tests
EUR 225

Apex Red Taq DNA Polymerase, 10000u

42-402R 20 x 500u/Unit
EUR 1229.49
Description: 10X NH4 Buffer, Mg Free

Apex Red Taq DNA Polymerase, 500u

42-409R 1 x 500u/Unit
EUR 62.1
Description: 10X NH4 Buffer, Mg Free

Taq HS Red DNA Polymerase

abx461041-1096tests 10 × 96 tests Ask for price

Taq HS Red DNA Polymerase

abx461041-596tests 5 × 96 tests
EUR 2187.5

Taq HS Red DNA Polymerase

abx461041-96tests 96 tests
EUR 1062.5

Recombinant Taq Dna Polymerase - EACH

27079804 EACH
EUR 132.3

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

Taq Plus DNA Polymerase (PCR)

G012 250 U (50 ul)
EUR 87.6

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
EUR 127.2

Taq DNA Polymerase Polyclonal Antibody

7018-100 each
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T each
EUR 175.2

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

Taq DNA Polymerase with Red Dye

abx461047-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase with Red Dye

abx461047-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase with Red Dye

abx461047-96tests 96 tests
EUR 362.5

HotStart Taq DNA Polymerase (5U/uL)

A4144-250U 250U
EUR 66
Description: Biotechnology

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U each
EUR 272.4

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 37.29

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 290

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 314.4

Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20600 5000U
EUR 49

AtMax Taq DNA Polymerase (2.5U/µl), 200U

PL4201 each Ask for price

AtMax Taq DNA Polymerase (2.5U/µl), 500U

PL4202 each Ask for price

Chromo Taq DNA Polymerase (1U/µl), 200U

PL1205 each Ask for price

Chromo Taq DNA Polymerase (1U/µl), 500U

PL1206 each Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U each
EUR 126

Taq HS DNA Polymerase (Glycerol-free)

QL101-01 1,000 U
EUR 89

Taq HS DNA Polymerase (Glycerol-free)

QL101-02 5,000 U
EUR 406

Taq HS DNA Polymerase (Glycerol-free)

QL101-03 50,000 U
EUR 3625

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 710.4

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 276

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 393.6

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq Plus DNA Polymerase Recombinant

rAP-1063 Inquiry Ask for price

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1388.4

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 6540

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA Polymerase M101 (5,000 U/mL)

RK26000 250U
EUR 60

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 176.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 273.6

DNA Amplification Kit with Taq DNA Polymerase, 100app

PL1202-K each Ask for price

Taq DNA Polymerase with Red Dye Mix

abx461048-1096tests 10 × 96 tests Ask for price

Taq DNA Polymerase with Red Dye Mix

abx461048-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase with Red Dye Mix

abx461048-96tests 96 tests
EUR 325

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 896.24

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 129.6

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

ACTaq™ Taq Blue DNA Polymerase, 1000U

E2100B 1000U
EUR 153.24

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 157.2

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-100 2x500 units
EUR 230.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-200 4x500 units
EUR 375.6

Genorise® CLT Taq DNA Polymerase (1000 U)

GR108003 1000 U
EUR 199

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-1 500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-2 2500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-3 5000 Unit (s) Ask for price

Cheetah™ Hot Start Taq DNA Polymerase

29050 500EU
EUR 277
Description: Recombinant Taq polymerase from E. coli

Cheetah™ Hot Start Taq DNA Polymerase

29050-1 EA
EUR 277

HS Taq DNA Polymerase M101 (5,000 U /mL)

RK26201 1000U
EUR 39

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 135
Description: Intact Genomics Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends. Intact Genomics Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.Product Includes:Taq DNA Polymerase 2x Premix with Dye5x Magic Enhancer

Taq DNA Polymerase 2x Premix - 1000 Reactions

3250 1000
EUR 255
Description: Intact Genomics Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends. Intact Genomics Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.Product Includes:Taq DNA Polymerase 2x Premix with Dye5x Magic Enhancer

DNA Amplification Kit with Max Taq DNA Polymerase, 100app

PL2202-K each Ask for price

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 196.8

DNA Amplification Kit with Chromo Taq DNA Polymerase, 100app

PL1206-K each Ask for price

Anti-Taq Mix (Mixture of Antibodies to Taq DNA Polymerase

S136 1000 µg
EUR 270

Anti-Taq Mix (Mixture of Antibodies to Taq DNA Polymerase

S136L 5000 µg
EUR 899

Anti-Taq Mix (Mixture of Antibodies to Taq DNA Polymerase

S136XL 10000 µg
EUR 1550

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 198

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 360

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 699.6

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-100 100,000 units
EUR 3462

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-1096tests 10 × 96 tests
EUR 2187.5

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-596tests 5 × 96 tests
EUR 1062.5

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-96tests 96 tests
EUR 400

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-010 100 units
EUR 180

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3

P0330-020 2x100 units
EUR 286.8

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-050 5X100 units
EUR 465.6

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-250 25X100 units
EUR 2004

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-500 50X100 units
EUR 3591.6

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 490.8

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 490.8

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 420.65

Taq DNA Polymerase - 1000 units with separate dNTP Mix

3243d 1000units
EUR 150
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

DNA Amplification Kit with Chromo Max Taq DNA Polymerase, 100app

PL2206-K each Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase

P950 100 units
EUR 59

Reversible 1-min Hot Start Taq DNA Polymerase

P950L 1,000 units
EUR 399

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 595
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer10mM dNTP Mix

Active Taq Polymerase (Taq)

APX161Ge61 10ug
EUR 280

Taq Polymerase

PCR-211L 5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-211S 200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-420-10KU 10kilo units
EUR 1043.5

Taq Polymerase

PCR-420-1KU 1kilo unit
EUR 222.3

Taq Polymerase

MBT060-1000U 1 unit
EUR 52.73
Description: Taq Polymerase

Taq Polymerase

MBT060-5000U 1 unit
EUR 204.76
Description: Taq Polymerase

However, phosphatase treatment is less effective for gap repair cloning rather than in vitro ligation, probably due to the ability of S. cerevisiae DNA repair machinery to efficiently repair the lost phosphate groups to allow religation. We have developed a more efficient method to prevent vector religation, the vector fragment by treatment with Taq DNA polymerase and dATP. It prevents the procedure recircularization vector almost completely, to facilitate the screening of recombinant clones correctly.

Leave A Comment