Reduced reaction volumes and increased Taq DNA polymerase concentration improve STR profiling outcomes from a real-world low template DNA source: telogen hairs.

Reduced reaction volumes and increased Taq DNA polymerase concentration improve STR profiling outcomes from a real-world low template DNA source: telogen hairs.

OBJECTIVE
The main method for the analysis of low template DNA (LTDNA) is known as a method of low copy number (LCN), which involves increasing the number of PCR cycles (typically 34). In common with other LTDNA method, characterized by an imbalance LCN profile allele, decline, and the drop outs which require interpretation of complex rules. They often require replicate PCR reactions to produce a “consensus” profile in special facilities. The ideal method for the analysis of LTDNA should raise the profile of the results without a high error rate and is accomplished using the standard amenities, in addition to the minimum cost.


METHOD
In this study, we present a comparison of four methods of variation for amplification of STR from LTDNA broadly used, the kit is available commercially (AmpFℓSTR (®) Profiler Plus (®)): the standard method, the standard method to post a PCR clean up, the method of the LCN, and the reaction volume decreases with increased concentrations of Taq DNA polymerase.


RESULTS
Using the telogen hair-LTDNA common source and reference DNA match, LCN method produces the highest number of appropriate and non-appropriate alleles (ie, down-in). For comparison, the reaction volume was reduced by an increase in Taq Polymerase produce fuller profile and corresponding DNA (all alleles combined) and less off-ladder alleles of a variety of input DNA. In addition, this method results in less non-concordant allele of the LCN and not more than for standard PCR, which indicates that it may be preferable to increase PCR cycles for analysis LTDNA, either with or without consensus profiles and statistical modeling.


CONCLUSION
Overall, this study highlights the importance and benefits of optimizing PCR conditions and to develop improved laboratory method to amplify and analyze LTDNA.

 Reduced reaction volumes and increased Taq DNA polymerase concentration improve STR profiling outcomes from a real-world low template DNA source: telogen hairs.
Reduced reaction volumes and increased Taq DNA polymerase concentration improve STR profiling outcomes from a real-world low template DNA source: telogen hairs.

[Effect of Incorporation Efficiency Charge Chromophore on fluorescently-labeled nucleotides in Matrix synthesis by Taq DNA Polymerase].

In order to study the effect of the electric charge of the chromophore, the efficiency of incorporation of fluorescently labeled nucleotides into DNA during PCR, three fluorescently labeled dUPT, one with dye electroneutral and the other two with positive and negative (Cy5 analog), was synthesized.

This shows that dUPT, labeled with Cy 5 electroneutral analog, most effectively incorporated into the DNA when Tag polymerase used for PCR.
Polymerase chain reaction (PCR) is widely used in a variety of experimental conditions, and Taq DNA polymerase is crucial in the process of PCR. In this article, Taq DNA expression polymerase plasmid reconstructed and protein products obtained by purification fast, ( “Fast purification of high-activity of Taq DNA polymerase” (Pluthero, 1993 [1]), “Single-step purification of DNA thermostable polymerase expressed in Escherichia coli “(Desai and Pfaffle, 1995 [2])). Here we present data on production of protein expression and provides the analysis result of the production of two different vectors. Meanwhile, refining the data is also provided to indicate the purity of the protein product.

Taq Polymerase(1U/µl)

MBT060D-500U 1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060E-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060E-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060E-500U 1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)

Taq DNA Polymerase (5U/µl), 2000U

PL1204 each Ask for price

Taq DNA Polymerase (5U/µl), 500U

PL1202 each Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

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AtMax Taq DNA Polymerase (2.5U/µl), 200U

PL4201 each Ask for price

AtMax Taq DNA Polymerase (2.5U/µl), 500U

PL4202 each Ask for price

Chromo Pfu DNA Polymerase (1U/µl), 100U

PL5205 each Ask for price

Chromo Pfu DNA Polymerase (1U/µl), 500U

PL5206 each Ask for price

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Taq DNA Polymerase

G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA Polymerase

L7051001 1000 unit
EUR 167

Taq DNA Polymerase

L7051200 200 unit
EUR 43

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Chromo AtTaq DNA Polymerase (1U/µl), 200U

PL3205 each Ask for price

Chromo AtTaq DNA Polymerase (1U/µl), 500U

PL3206 each Ask for price

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PL2205 each Ask for price

Chromo MaxTaq DNA Polymerase (1U/µl), 500U

PL2206 each Ask for price

Taq Polymerase (5U/µl)

MBT060A-1000U 1 unit
EUR 62.03
Description: Taq Polymerase (5U/µl)

Taq Polymerase (5U/µl)

MBT060A-5000U 1 unit
EUR 241.01
Description: Taq Polymerase (5U/µl)

Taq Polymerase (5U/µl)

MBT060A-500U 1 unit
EUR 41.58
Description: Taq Polymerase (5U/µl)

Taq Polymerase(3U/µl)

MBT060B-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060B-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060B-500U 1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-500U 1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S111 500 units
EUR 63.6

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S112 5x500 units
EUR 148.8

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S128 20x500 units
EUR 487.2

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S129 100x500 units or as bulk
EUR 2142

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

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9K-001-0033 6000U
EUR 1234.5

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9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

Taq HS DNA Polymerase

P132-d1 500 U
EUR 47.85

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 89.18

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 406

TM Taq DNA Polymerase

TBS4010 1000 U
EUR 60

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

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G126 10000 U (2 x 1.0 ml)
EUR 370.8

Taq DNA Polymerase Mix

abx461043-1096tests 10 × 96 tests Ask for price

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abx461043-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase Mix

abx461043-96tests 96 tests
EUR 337.5

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase

S101 500 units
EUR 55.2

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 128.4

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 393.6

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 595

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 167

Taq DNA Polymerase -1000 units

3243 1000units
EUR 115
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 449
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Taq DNA Polymerase (Recombinant)

22060707-1 1,000 Unit(s)
EUR 137.27

Taq DNA Polymerase (Recombinant)

22060707-2 1500 Unit(s)
EUR 182.1

Taq DNA Polymerase (Recombinant)

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EUR 465.77

Taq DNA Polymerase (Recombinant)

22060707-4 10 KU
EUR 840.96

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 607.62

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA Polymerase Recombinant

rAP-1062 Inquiry Ask for price

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

Taq DNA Polymerase (5U/uL)

A1003-500U 500U
EUR 26.4
Description: Biotechnology

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

Taq Plus DNA Polymerase (Taq Plus DNA) Protein

abx073522-100tests 100 tests
EUR 225

Hot start Taq DNA Polymerase

EQ006 100μl/500U
EUR 200

Hot Start Taq DNA Polymerase

abx460010-1096tests 10 × 96 tests Ask for price

Hot Start Taq DNA Polymerase

abx460010-596tests 5 × 96 tests Ask for price

Hot Start Taq DNA Polymerase

abx460010-96tests 96 tests
EUR 262.5

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

Taq DNA Polymerase (TaqDNA) Protein

abx073521-100tests 100 tests
EUR 225

Apex Red Taq DNA Polymerase, 10000u

42-402R 20 x 500u/Unit
EUR 1229.49
Description: 10X NH4 Buffer, Mg Free

Apex Red Taq DNA Polymerase, 500u

42-409R 1 x 500u/Unit
EUR 62.1
Description: 10X NH4 Buffer, Mg Free

Taq HS Red DNA Polymerase

abx461041-1096tests 10 × 96 tests Ask for price

Taq HS Red DNA Polymerase

abx461041-596tests 5 × 96 tests
EUR 2187.5

Taq HS Red DNA Polymerase

abx461041-96tests 96 tests
EUR 1062.5

Recombinant Taq Dna Polymerase - EACH

27079804 EACH
EUR 132.3

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

Taq Plus DNA Polymerase (PCR)

G012 250 U (50 ul)
EUR 87.6

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
EUR 127.2

Taq DNA Polymerase Polyclonal Antibody

7018-100 each
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T each
EUR 175.2

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

Taq DNA Polymerase with Red Dye

abx461047-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase with Red Dye

abx461047-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase with Red Dye

abx461047-96tests 96 tests
EUR 362.5

HotStart Taq DNA Polymerase (5U/uL)

A4144-250U 250U
EUR 66
Description: Biotechnology

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U each
EUR 272.4

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 37.29

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 290

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 314.4

Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20600 5000U
EUR 49

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U each
EUR 126

Taq HS DNA Polymerase (Glycerol-free)

QL101-01 1,000 U
EUR 89

Taq HS DNA Polymerase (Glycerol-free)

QL101-02 5,000 U
EUR 406

Taq HS DNA Polymerase (Glycerol-free)

QL101-03 50,000 U
EUR 3625

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 710.4

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 276

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 393.6

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq Plus DNA Polymerase Recombinant

rAP-1063 Inquiry Ask for price

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1388.4

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 6540

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA Polymerase M101 (5,000 U/mL)

RK26000 250U
EUR 60

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 176.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 273.6

DNA Amplification Kit with Taq DNA Polymerase, 100app

PL1202-K each Ask for price

Taq DNA Polymerase with Red Dye Mix

abx461048-1096tests 10 × 96 tests Ask for price

Taq DNA Polymerase with Red Dye Mix

abx461048-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase with Red Dye Mix

abx461048-96tests 96 tests
EUR 325

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 896.24

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 129.6

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

ACTaq™ Taq Blue DNA Polymerase, 1000U

E2100B 1000U
EUR 153.24

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 157.2

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-100 2x500 units
EUR 230.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-200 4x500 units
EUR 375.6

Genorise® CLT Taq DNA Polymerase (1000 U)

GR108003 1000 U
EUR 199

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-1 500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-2 2500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-3 5000 Unit (s) Ask for price

Cheetah™ Hot Start Taq DNA Polymerase

29050 500EU
EUR 277
Description: Recombinant Taq polymerase from E. coli

Cheetah™ Hot Start Taq DNA Polymerase

29050-1 EA
EUR 277

HS Taq DNA Polymerase M101 (5,000 U /mL)

RK26201 1000U
EUR 39

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 135
Description: Intact Genomics Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends. Intact Genomics Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.Product Includes:Taq DNA Polymerase 2x Premix with Dye5x Magic Enhancer

Taq DNA Polymerase 2x Premix - 1000 Reactions

3250 1000
EUR 255
Description: Intact Genomics Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends. Intact Genomics Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.Product Includes:Taq DNA Polymerase 2x Premix with Dye5x Magic Enhancer

DNA Amplification Kit with Max Taq DNA Polymerase, 100app

PL2202-K each Ask for price

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 196.8

DNA Amplification Kit with Chromo Taq DNA Polymerase, 100app

PL1206-K each Ask for price

Anti-Taq Mix (Mixture of Antibodies to Taq DNA Polymerase

S136 1000 µg
EUR 270

Anti-Taq Mix (Mixture of Antibodies to Taq DNA Polymerase

S136L 5000 µg
EUR 899

Anti-Taq Mix (Mixture of Antibodies to Taq DNA Polymerase

S136XL 10000 µg
EUR 1550

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 198

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 360

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 699.6

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-100 100,000 units
EUR 3462

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-1096tests 10 × 96 tests
EUR 2187.5

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-596tests 5 × 96 tests
EUR 1062.5


chemical modifications to DNA, such as modification 2 ‘, is expected to increase the utility of DNA biotechnology; However, other forms of modification of DNA is limited by their inability to effectively synthesized by a DNA polymerase enzyme. Previous efforts have identified a mutant Thermus aquaticus DNA polymerase I (Taq) enzyme that can recognize DNA 2’-modified nucleotides. While these modified nucleotides recognize the mutant enzyme, they are unable to synthesize the full length of the modified DNA; thus, further techniques necessary for this enzyme

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