A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.

Polymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleic acids DNA or RNA strand using the base-pairing interactions. One of the polymerase enzyme, Taq DNA polymerase, originally isolated from Thermus aquaticus (Taq) is an enzyme widely used in molecular biology so far. The properties of thermostable enzymes has contributed majorly to specificity, automation, and efficacy of the polymerase chain reaction (PCR), making it a powerful tool for molecular biology research today throughout the world.

Purification of Taq DNA polymerase from the original host results in low yields, labor and time consumption. Therefore, much research has been done before to get this enzyme using an alternative host. So far, all of the existing methodology is more laborious, time consuming and requires a heavy cost. We use a new approach to purifying the enzyme with relatively high efficiency, yield and minimum time consumption using Escherichia coli (E. coli) as an alternative host. We cloned 2500 base pairs of DNA Taq polymerase gene into pGEX-4T-1 vector containing GST-tag, downstream of tac promoter and expressed using isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer

The enzyme was purified using chromatography efficient new approach and use in routine PCR tests in our laboratory. Our findings suggest a new approach to facilitate the availability of polymerases for molecular and diagnostic studies. In the future, may be used for purification of other recombinant peptides or proteins used in structural biology and proteomics research-based.

 A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.

Outside Chelation: EDTA binds Closely Taq DNA Polymerase, Mutt and dUTPase and direct Inhibiting Activity dNTPase.

EDTA is commonly used as a metal ion chelator efficient enzyme cofactor. It is highly soluble, optically inactive and do not interfere with most chemicals used in making standard buffer EDTA common options for generating metal-free conditions for biochemical and biophysical investigations. However, controversy in the literature about the activity of the metal-free enzyme is achieved by using EDTA or otherwise called our attention to the alleged effects of external EDTA chelation. Here, we show that EDTA compete for sites of hydrolases dUTPase nucleotide nucleotide binding by developing a network of interactions within the active site similar to the substrate. To reach these findings, we apply the kinetics and molecular docking technique uses two different dUTPases.

Furthermore, we directly measured the binding of EDTA to dUTPases and two other dNTPases, Taq polymerase and Mutt using isothermal titration calorimetry. EDTA binds proven exothermic and enthalpy driven primarily by submicromolar dissociation constant is much lower than with an enzyme: substrate or Mg: EDTA complex. Control of protein, including ATPase, do not interact with EDTA. Our findings indicate that EDTA can act as a selective inhibitor of the enzyme hydrolysis of dNTPs and urges a rethinking of the use of EDTA in enzymatic trial.

TAQ DNA Polymerase (5U/mcl) (w/6X Buffer, w/dNTP)

51613-1 1000 Units
EUR 43.79
Description: Part E

TAQ DNA Polymerase (5U/mcl) (w/6X Buffer, w/dNTP)

51613-2 5000 Units
EUR 199.11
Description: Part E

Taq DNA Polymerase without Buffer

MBS308027-1000Units 1000Units
EUR 225

Taq DNA Polymerase without Buffer

MBS308027-100Units 100Units
EUR 120

Taq DNA Polymerase without Buffer

MBS308027-500Units 500Units
EUR 170

Taq DNA Polymerase (Buffer with Mgcl2)

MBS355980-1000Units 1000Units
EUR 240

Taq DNA Polymerase (Buffer with Mgcl2)

MBS355980-3000Units 3000Units
EUR 315

Taq DNA Polymerase (Buffer with Mgcl2)

MBS355980-500Units 500Units
EUR 215

Taq DNA Polymerase (Buffer with Mgcl2)

MBS355980-5x3000Units 5x3000Units
EUR 1110

Taq DNA Polymerase (Mg Plus Buffer)

MBS9141663-1000Units 1000Units
EUR 195

Taq DNA Polymerase (Mg Plus Buffer)

MBS9141663-10KUnits 10KUnits
EUR 995

Taq DNA Polymerase (Mg Plus Buffer)

MBS9141663-5000Units 5000Units
EUR 545

Taq DNA Polymerase (Buffer without Mgcl2)

MBS355981-1000Units 1000Units
EUR 240

Taq DNA Polymerase (Buffer without Mgcl2)

MBS355981-3000Units 3000Units
EUR 305

Taq DNA Polymerase (Buffer without Mgcl2)

MBS355981-500Units 500Units
EUR 210

Taq DNA Polymerase (Buffer without Mgcl2)

MBS355981-5x3000Units 5x3000Units
EUR 1080

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 37.29

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 290

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 314.4

Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20600 5000U
EUR 49

Taq DNA Polymerase (Mg2+ plus buffer)

P101-01-1000U 1,000 U
EUR 38.61

Taq DNA Polymerase (Mg2+ plus buffer)

P101-03-10000U 10,000 U
EUR 300.3

Taq DNA Polymerase (5U/µl), 2000U

PL1204 each Ask for price

Taq DNA Polymerase (5U/µl), 500U

PL1202 each Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-1 500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-2 2500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-3 5000 Unit (s) Ask for price

HotStart Taq DNA Polymerase (Mg Plus Buffer)

MBS9141664-200Units 200Units
EUR 150

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

Taq (exo-) DNA Polymerase (Mg2+ Plus Buffer)

RK20610 5000U
EUR 190.75

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 490.8

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 490.8

Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer

MBS308022-1000Units 1000Units
EUR 225

Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer

MBS308022-100Units 100Units
EUR 135

Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer

MBS308022-500Units 500Units
EUR 170

Taq Polymerase (5U/µl)

MBT060A-1000U 1 unit
EUR 62.03
Description: Taq Polymerase (5U/µl)

Taq Polymerase (5U/µl)

MBT060A-5000U 1 unit
EUR 241.01
Description: Taq Polymerase (5U/µl)

Taq Polymerase (5U/µl)

MBT060A-500U 1 unit
EUR 41.58
Description: Taq Polymerase (5U/µl)

HotStart Taq (exo-) DNA Polymerase (Mg2+ Plus Buffer)

RK20611 1000U
EUR 501.4

Taq DNA Polymerase with 10X Reaction (No MgCl2) Buffer

MBS308028-1000Units 1000Units
EUR 225

Taq DNA Polymerase with 10X Reaction (No MgCl2) Buffer

MBS308028-100Units 100Units
EUR 120

Taq DNA Polymerase with 10X Reaction (No MgCl2) Buffer

MBS308028-500Units 500Units
EUR 170

Taq Polymerase / Labeling Buffer

PCR-201L 5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase / Labeling Buffer

PCR-201S 200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli

FSP TAQ DNA Polymerase (5U/mcl) (w/dNTP)

70073 250 Units
EUR 33.1
Description: Part E

FSP TAQ DNA Polymerase (5U/mcl) (w/dNTP)

70073-1 1000 Units
EUR 101.09
Description: Part E

Long TAQ DNA Polymerase (5U/mcl) (w/dNTP)

94126 250 Units
EUR 42.76
Description: Part E

Long TAQ DNA Polymerase (5U/mcl) (w/dNTP)

94126-1 1000 Units
EUR 128.29
Description: Part E

Taq DNA Polymerase (5U/uL)

A1003-500U 500U
EUR 26.4
Description: Biotechnology

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

TAQ DNA Polymerase (5U/mcl)

82487 500 Units
EUR 19.5
Description: Part E

TAQ DNA Polymerase (5U/mcl)

82487-1 1000 Units
EUR 36.95
Description: Part E

TAQ DNA Polymerase (5U/mcl)

82487-2 5000 Units
EUR 179.69
Description: Part E

Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2

11140016-1 500 Unit(s)
EUR 108.39

Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2

11140016-2 2500 Unit(s)
EUR 490.36

Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2

11140016-3 5000 Unit(s)
EUR 912.79

HSP TAQ DNA Polymerase (5U/mcl)

46033 500 Units
EUR 40.88
Description: Part E

HSP TAQ DNA Polymerase (5U/mcl)

46033-1 1000 Units
EUR 75.78
Description: Part E

FSP TAQ DNA Polymerase (5U/mcl)

58235 250 Units
EUR 31.13
Description: Part E

FSP TAQ DNA Polymerase (5U/mcl)

58235-1 1000 Units
EUR 97.16
Description: Part E

Long TAQ DNA Polymerase (5U/mcl)

15367 250 Units
EUR 28.22
Description: Part E

Long TAQ DNA Polymerase (5U/mcl)

15367-1 1000 Units
EUR 102.63
Description: Part E

HotStart Taq DNA Polymerase (5U/uL)

A4144-250U 250U
EUR 66
Description: Biotechnology

Roseate Taq DNA Polymerase (5U/mcl)

12192 500 Units
EUR 25.91
Description: Part E

Roseate Taq DNA Polymerase (5U/mcl)

12192-1 1000 Units
EUR 42.59
Description: Part E

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

DNA Polymerase Reaction Buffer

abx461036-1096tests 10 × 96 tests Ask for price

DNA Polymerase Reaction Buffer

abx461036-596tests 5 × 96 tests Ask for price

DNA Polymerase Reaction Buffer

abx461036-96tests 96 tests
EUR 562.5

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 896.24

Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 129.6

AtMax Taq DNA Polymerase (2.5U/µl), 200U

PL4201 each Ask for price

AtMax Taq DNA Polymerase (2.5U/µl), 500U

PL4202 each Ask for price

Chromo Taq DNA Polymerase (1U/µl), 200U

PL1205 each Ask for price

Chromo Taq DNA Polymerase (1U/µl), 500U

PL1206 each Ask for price

Pfu DNA Polymerase (5U/µl), 100U

PL5201 each Ask for price

Pfu DNA Polymerase (5U/µl), 500U

PL5202 each Ask for price

AtTaq DNA Polymerase (5U/µl), 200U

PL3201 each Ask for price

AtTaq DNA Polymerase (5U/µl), 500U

PL3202 each Ask for price

h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl

11140022-1 250 Unit (s) Ask for price

h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl

11140022-2 1000 Unit (s) Ask for price

MaxTaq DNA Polymerase (5U/µl), 200U

PL2201 each Ask for price

MaxTaq DNA Polymerase (5U/µl), 500U

PL2202 each Ask for price

Pfu-fast DNA Polymerase (Mg2+ Plus Buffer)

RK20650 5000U
EUR 65.4

Deep Ocean DNA Polymerase (Mg2+ Plus Buffer)

RK20660 1000U
EUR 667.63

Long-Taq DNA Polymerase (5U/uL)

A4186-250U 250U
EUR 66
Description: Biotechnology

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-01 125 U
EUR 145.2

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-02 500 U
EUR 210

FlashTaq HOTSTART DNA Polymerase without Buffer

MBS308007-1000Units 1000Units
EUR 320

FlashTaq HOTSTART DNA Polymerase without Buffer

MBS308007-100Units 100Units
EUR 145

FlashTaq HOTSTART DNA Polymerase without Buffer

MBS308007-500Units 500Units
EUR 255

Deep Ocean (exo-) DNA Polymerase (Mg2+ Plus Buffer)

RK20670 200U
EUR 147.15

Taq Polymerase(3U/µl)

MBT060B-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060B-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060B-500U 1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-500U 1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)

Taq Polymerase(1U/µl)

MBT060D-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060D-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060D-500U 1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060E-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060E-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060E-500U 1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

E2500-01 200U
EUR 31.61
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-02 1000U
EUR 120.99
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-03 5000U
EUR 599.5
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-04 500U
EUR 69.76
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

EK2500-01 200U
EUR 40.33
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-02 1000U
EUR 163.5
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-03 5000U
EUR 809.87
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-04 500U
EUR 90.47
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EQ005-100ul500U 100μl/500U
EUR 15

Taq DNA Polymerase

EQ005-500ul2500U 500μl/2500U
EUR 50

Taq DNA Polymerase

G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Taq DNA Polymerase

L7051001 1000 unit
EUR 167

Taq DNA Polymerase

L7051200 200 unit
EUR 43

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

MBS157418-1000Units 1000Units
EUR 190

Taq DNA Polymerase

MBS157418-3000Units 3000Units
EUR 245

Taq DNA Polymerase

MBS157418-500Units 500Units
EUR 175

Taq DNA Polymerase

MBS157418-5x3000Units 5x3000Units
EUR 780

Taq DNA Polymerase

MBS402024-500Units 500Units
EUR 170

Taq DNA Polymerase

MBS402024-50Units 50Units
EUR 160

T7 RNA Polymerase (50U/ul) (w/10X IVT Buffer)

98343 1000 Units
EUR 68.42
Description: Part E

T7 RNA Polymerase (50U/ul) (w/10X IVT Buffer)

98343-1 5000 Units
EUR 333.55
Description: Part E

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d1 125 U
EUR 147.6

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d2 500 U
EUR 213.6

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d1 125 U
EUR 147.6

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d2 500 U
EUR 213.6

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

Taq HS DNA Polymerase

P132-d1 500 U
EUR 47.85

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 89.18

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 406

TM Taq DNA Polymerase

TBS4010 1000 U
EUR 60

Taq HS DNA Polymerase

P132-d1-500U 500 U
EUR 49.55

Taq HS DNA Polymerase

P132-d2-1000U 1,000 U
EUR 92.34

Taq HS DNA Polymerase

P132-d3-5000U 5,000 U
EUR 420.41

HLA Taq DNA Polymerase

E2717-01 200U
EUR 37.06
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits

HLA Taq DNA Polymerase

E2717-02 1000U
EUR 146.06
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits

HLA Taq DNA Polymerase

E2717-03 5000U
EUR 719.4
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits

HLA Taq DNA Polymerase

E2717-04 500U
EUR 82.84
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

Taq DNA Polymerase Mix

abx461043-1096tests 10 × 96 tests Ask for price

Taq DNA Polymerase Mix

abx461043-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase Mix

abx461043-96tests 96 tests
EUR 337.5

Hot Start Taq Polymerase (5U/mcl)

81646 250 Units
EUR 66.11
Description: Part E

Hot Start Taq Polymerase (5U/mcl)

81646-1 1000 Units
EUR 252.56
Description: Part E

Long Taq DNA Polymerase

MBS355971-3000Units 3000Units
EUR 445

Long Taq DNA Polymerase

MBS355971-500Units 500Units
EUR 235

Long Taq DNA Polymerase

MBS355971-5x3000Units 5x3000Units
EUR 1700

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Color Taq DNA Polymerase

E2510-01 200U
EUR 31.61
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)

Color Taq DNA Polymerase

E2510-02 1000U
EUR 120.99
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)

Color Taq DNA Polymerase

E2510-03 5000U
EUR 599.5
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)

Color Taq DNA Polymerase

E2510-04 500U
EUR 69.76
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)

Color Taq DNA Polymerase

EK2510-01 200U
EUR 40.33
Description: Color Taq DNA Polymerase Kit containing polymerase and dNTPs


In order to develop a test to identify the genes responsible for antibiotic resistance in gram-negative bacteria, it has been found that the standard of Taq DNA polymerase (not DNA-free) blaTEM contaminated with fragments of genes that varied in length and number. BlaTEM complete gene sequences are absent or detected in very small amounts. We developed an approach to avoid false-positive findings caused by contamination polymerase gene sequence in a conventional blaTEM