Polymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleic acids DNA or RNA strand using the base-pairing interactions. One of the polymerase enzyme, Taq DNA polymerase, originally isolated from Thermus aquaticus (Taq) is an enzyme widely used in molecular biology so far. The properties of thermostable enzymes has contributed majorly to specificity, automation, and efficacy of the polymerase chain reaction (PCR), making it a powerful tool for molecular biology research today throughout the world.
Purification of Taq DNA polymerase from the original host results in low yields, labor and time consumption. Therefore, much research has been done before to get this enzyme using an alternative host. So far, all of the existing methodology is more laborious, time consuming and requires a heavy cost. We use a new approach to purifying the enzyme with relatively high efficiency, yield an d minimum time consumption using Escherichia coli (E. coli) as an alternative host. We cloned 2500 base pairs of DNA Taq polymerase gene into pGEX-4T-1 vector containing GST-tag, downstream of tac promoter and expressed using isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer
The enzyme was purified using chromatography efficient new approach and use in routine PCR tests in our laboratory. Our findings suggest a new approach to facilitate the availability of polymerases for molecular and diagnostic studies. In the future, may be used for purification of other recombinant peptides or proteins used in structural biology and proteomics research-based.
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.
Outside Chelation: EDTA binds Closely Taq DNA Polymerase, Mutt and dUTPase and direct Inhibiting Activity dNTPase.
EDTA is commonly used as a metal ion chelator efficient enzyme cofactor. It is highly soluble, optically inactive and do not interfere with most chemicals used in making standard buffer EDTA common options for generating metal-free conditions for biochemical and biophysical investigations. However, controversy in the literature about the activity of the metal-free enzyme is achieved by using EDTA or otherwise called our attention to the alleged effects of external EDTA chelation. Here, we show that EDTA compete for sites of hydrolases dUTPase nucleotide nucleotide binding by developing a network of interactions within the active site similar to the substrate. To reach these findings, we apply the kinetics and molecular docking technique uses two different dUTPases.
Furthermore, we directly measured the binding of EDTA to dUTPases and two other dNTPases, Taq polymerase and Mutt using isothermal titration calorimetry. EDTA binds proven exothermic and enthalpy driven primarily by submicromolar dissociation constant is much lower than with an enzyme: substrate or Mg: EDTA complex. Control of protein, including ATPase, do not interact with EDTA. Our findings indicate that EDTA can act as a selective inhibitor of the enzyme hydrolysis of dNTPs and urges a rethinking of the use of EDTA in enzymatic trial.
TAQ DNA Polymerase (5U/mcl) (w/6X Buffer, w/dNTP)
51613-1
Sisco Laboratories
1000 Units
EUR 43.79
Description: Part E
TAQ DNA Polymerase (5U/mcl) (w/6X Buffer, w/dNTP)
51613-2
Sisco Laboratories
5000 Units
EUR 199.11
Description: Part E
Taq DNA Polymerase without Buffer
MBS308027-1000Units
MyBiosource
1000Units
EUR 225
Taq DNA Polymerase without Buffer
MBS308027-100Units
MyBiosource
100Units
EUR 120
Taq DNA Polymerase without Buffer
MBS308027-500Units
MyBiosource
500Units
EUR 170
Taq DNA Polymerase (Buffer with Mgcl2)
MBS355980-1000Units
MyBiosource
1000Units
EUR 240
Taq DNA Polymerase (Buffer with Mgcl2)
MBS355980-3000Units
MyBiosource
3000Units
EUR 315
Taq DNA Polymerase (Buffer with Mgcl2)
MBS355980-500Units
MyBiosource
500Units
EUR 215
Taq DNA Polymerase (Buffer with Mgcl2)
MBS355980-5x3000Units
MyBiosource
5x3000Units
EUR 1110
Taq DNA Polymerase (Mg Plus Buffer)
MBS9141663-1000Units
MyBiosource
1000Units
EUR 195
Taq DNA Polymerase (Mg Plus Buffer)
MBS9141663-10KUnits
MyBiosource
10KUnits
EUR 995
Taq DNA Polymerase (Mg Plus Buffer)
MBS9141663-5000Units
MyBiosource
5000Units
EUR 545
Taq DNA Polymerase (Buffer without Mgcl2)
MBS355981-1000Units
MyBiosource
1000Units
EUR 240
Taq DNA Polymerase (Buffer without Mgcl2)
MBS355981-3000Units
MyBiosource
3000Units
EUR 305
Taq DNA Polymerase (Buffer without Mgcl2)
MBS355981-500Units
MyBiosource
500Units
EUR 210
Taq DNA Polymerase (Buffer without Mgcl2)
MBS355981-5x3000Units
MyBiosource
5x3000Units
EUR 1080
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-01
Vazyme
1000 U
EUR 37.29
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-02
Vazyme
5000 U
EUR 231.6
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-03
Vazyme
10000 U
EUR 290
Taq DNA Polymerase (Mg2+ free Buffer)
P102-01
Vazyme
1000 U
EUR 145.2
Taq DNA Polymerase (Mg2+ free Buffer)
P102-02
Vazyme
5000 U
EUR 231.6
Taq DNA Polymerase (Mg2+ free Buffer)
P102-03
Vazyme
10000 U
EUR 314.4
Taq DNA Polymerase (Mg2+ Plus Buffer)
RK20600
Abclonal
5000U
EUR 49
Taq DNA Polymerase (Mg2+ plus buffer)
P101-01-1000U
Vazyme
1,000 U
EUR 38.61
Taq DNA Polymerase (Mg2+ plus buffer)
P101-03-10000U
Vazyme
10,000 U
EUR 300.3
Taq DNA Polymerase (5U/µl), 2000U
PL1204
Vivantis
each
Ask for price
Taq DNA Polymerase (5U/µl), 500U
PL1202
Vivantis
each
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Taq DNA Polymerase with dNTP Mix Buffer
11140015-1
Bio-WORLD
500 Unit (s)
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Taq DNA Polymerase with dNTP Mix Buffer
11140015-2
Bio-WORLD
2500 Unit (s)
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Taq DNA Polymerase with dNTP Mix Buffer
11140015-3
Bio-WORLD
5000 Unit (s)
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HotStart Taq DNA Polymerase (Mg Plus Buffer)
MBS9141664-200Units
MyBiosource
200Units
EUR 150
HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)
RK20601
Abclonal
25 U
Ask for price
Taq (exo-) DNA Polymerase (Mg2+ Plus Buffer)
RK20610
Abclonal
5000U
EUR 190.75
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d1
Vazyme
1000 U
EUR 163.2
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d2
Vazyme
5000 U
EUR 320.4
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d3
Vazyme
10000 U
EUR 490.8
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d1
Vazyme
1000 U
EUR 163.2
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d2
Vazyme
5000 U
EUR 320.4
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d3
Vazyme
10000 U
EUR 490.8
Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer
MBS308022-1000Units
MyBiosource
1000Units
EUR 225
Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer
MBS308022-100Units
MyBiosource
100Units
EUR 135
Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer
MBS308022-500Units
MyBiosource
500Units
EUR 170
Taq Polymerase (5U/µl)
MBT060A-1000U
EWC Diagnostics
1 unit
EUR 62.03
Description: Taq Polymerase (5U/µl)
Taq Polymerase (5U/µl)
MBT060A-5000U
EWC Diagnostics
1 unit
EUR 241.01
Description: Taq Polymerase (5U/µl)
Taq Polymerase (5U/µl)
MBT060A-500U
EWC Diagnostics
1 unit
EUR 41.58
Description: Taq Polymerase (5U/µl)
HotStart Taq (exo-) DNA Polymerase (Mg2+ Plus Buffer)
RK20611
Abclonal
1000U
EUR 501.4
Taq DNA Polymerase with 10X Reaction (No MgCl2) Buffer
MBS308028-1000Units
MyBiosource
1000Units
EUR 225
Taq DNA Polymerase with 10X Reaction (No MgCl2) Buffer
MBS308028-100Units
MyBiosource
100Units
EUR 120
Taq DNA Polymerase with 10X Reaction (No MgCl2) Buffer
MBS308028-500Units
MyBiosource
500Units
EUR 170
Taq Polymerase / Labeling Buffer
PCR-201L
Jena Bioscience GmbH
5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli
Taq Polymerase / Labeling Buffer
PCR-201S
Jena Bioscience GmbH
200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli
FSP TAQ DNA Polymerase (5U/mcl) (w/dNTP)
70073
Sisco Laboratories
250 Units
EUR 33.1
Description: Part E
FSP TAQ DNA Polymerase (5U/mcl) (w/dNTP)
70073-1
Sisco Laboratories
1000 Units
EUR 101.09
Description: Part E
Long TAQ DNA Polymerase (5U/mcl) (w/dNTP)
94126
Sisco Laboratories
250 Units
EUR 42.76
Description: Part E
Long TAQ DNA Polymerase (5U/mcl) (w/dNTP)
94126-1
Sisco Laboratories
1000 Units
EUR 128.29
Description: Part E
Taq DNA Polymerase, 5u/ul
B0089(D0089)
Bio Basic
5X200U
EUR 112.2
TAQ DNA Polymerase (5U/mcl)
82487
Sisco Laboratories
500 Units
EUR 19.5
Description: Part E
TAQ DNA Polymerase (5U/mcl)
82487-1
Sisco Laboratories
1000 Units
EUR 36.95
Description: Part E
TAQ DNA Polymerase (5U/mcl)
82487-2
Sisco Laboratories
5000 Units
EUR 179.69
Description: Part E
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-1
Glycomatrix
500 Unit(s)
EUR 108.39
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-2
Glycomatrix
2500 Unit(s)
EUR 490.36
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-3
Glycomatrix
5000 Unit(s)
EUR 912.79
HSP TAQ DNA Polymerase (5U/mcl)
46033
Sisco Laboratories
500 Units
EUR 40.88
Description: Part E
HSP TAQ DNA Polymerase (5U/mcl)
46033-1
Sisco Laboratories
1000 Units
EUR 75.78
Description: Part E
FSP TAQ DNA Polymerase (5U/mcl)
58235
Sisco Laboratories
250 Units
EUR 31.13
Description: Part E
FSP TAQ DNA Polymerase (5U/mcl)
58235-1
Sisco Laboratories
1000 Units
EUR 97.16
Description: Part E
Long TAQ DNA Polymerase (5U/mcl)
15367
Sisco Laboratories
250 Units
EUR 28.22
Description: Part E
Long TAQ DNA Polymerase (5U/mcl)
15367-1
Sisco Laboratories
1000 Units
EUR 102.63
Description: Part E
HotStart Taq DNA Polymerase (5U/uL)
A4144-250U
Biomatik Corporation
250U
EUR 66
Description: Biotechnology
Roseate Taq DNA Polymerase (5U/mcl)
12192
Sisco Laboratories
500 Units
EUR 25.91
Description: Part E
Roseate Taq DNA Polymerase (5U/mcl)
12192-1
Sisco Laboratories
1000 Units
EUR 42.59
Description: Part E
Taq Plus DNA Polymerase, 5u/ul
D0090
Bio Basic
200U
EUR 94.8
DNA Polymerase Reaction Buffer
abx461036-1096tests
Abbexa
10 × 96 tests
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DNA Polymerase Reaction Buffer
abx461036-596tests
Abbexa
5 × 96 tests
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DNA Polymerase Reaction Buffer
abx461036-96tests
Abbexa
96 tests
EUR 562.5
3G HotStart Taq DNA Polymerase (5u/ul)
3GHST81
Bio Basic
5x500U
EUR 896.24
Taq DNA Polymerase (High Purity), 5u/ul
HTD0078
Bio Basic
5X200U
EUR 129.6
Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ001
Allele Biotech
1000U
Ask for price
Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ100
Allele Biotech
100U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ200
Allele Biotech
200U
Ask for price
Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ500
Allele Biotech
500U
Ask for price
AtMax Taq DNA Polymerase (2.5U/µl), 200U
PL4201
Vivantis
each
Ask for price
AtMax Taq DNA Polymerase (2.5U/µl), 500U
PL4202
Vivantis
each
Ask for price
Chromo Taq DNA Polymerase (1U/µl), 200U
PL1205
Vivantis
each
Ask for price
Chromo Taq DNA Polymerase (1U/µl), 500U
PL1206
Vivantis
each
Ask for price
Pfu DNA Polymerase (5U/µl), 100U
PL5201
Vivantis
each
Ask for price
Pfu DNA Polymerase (5U/µl), 500U
PL5202
Vivantis
each
Ask for price
AtTaq DNA Polymerase (5U/µl), 200U
PL3201
Vivantis
each
Ask for price
AtTaq DNA Polymerase (5U/µl), 500U
PL3202
Vivantis
each
Ask for price
h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl
11140022-1
Bio-WORLD
250 Unit (s)
Ask for price
h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl
11140022-2
Bio-WORLD
1000 Unit (s)
Ask for price
MaxTaq DNA Polymerase (5U/µl), 200U
PL2201
Vivantis
each
Ask for price
MaxTaq DNA Polymerase (5U/µl), 500U
PL2202
Vivantis
each
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Pfu-fast DNA Polymerase (Mg2+ Plus Buffer)
RK20650
Abclonal
5000U
EUR 65.4
Deep Ocean DNA Polymerase (Mg2+ Plus Buffer)
RK20660
Abclonal
1000U
EUR 667.63
Long-Taq DNA Polymerase (5U/uL)
A4186-250U
Biomatik Corporation
250U
EUR 66
Description: Biotechnology
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-01
Vazyme
125 U
EUR 145.2
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-02
Vazyme
500 U
EUR 210
FlashTaq HOTSTART DNA Polymerase without Buffer
MBS308007-1000Units
MyBiosource
1000Units
EUR 320
FlashTaq HOTSTART DNA Polymerase without Buffer
MBS308007-100Units
MyBiosource
100Units
EUR 145
FlashTaq HOTSTART DNA Polymerase without Buffer
MBS308007-500Units
MyBiosource
500Units
EUR 255
Deep Ocean (exo-) DNA Polymerase (Mg2+ Plus Buffer)
RK20670
Abclonal
200U
EUR 147.15
Taq Polymerase(3U/µl)
MBT060B-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060B-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060B-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)
Taq Polymerase(1U/µl)
MBT060D-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060D-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060D-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)
Taq DNA Polymerase
9001-2500
Biovision
each
EUR 614.4
Taq DNA Polymerase
9001-500
Biovision
each
EUR 229.2
Taq DNA Polymerase
BA00103
Cusabio
500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00104
Bioatlas
1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00105
Bioatlas
2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10101
Bioatlas
500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10102
Bioatlas
1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10103
Bioatlas
2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
E2500-01
EURx
200U
EUR 31.61
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)
Taq DNA Polymerase
E2500-02
EURx
1000U
EUR 120.99
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)
Taq DNA Polymerase
E2500-03
EURx
5000U
EUR 599.5
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)
Taq DNA Polymerase
E2500-04
EURx
500U
EUR 69.76
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)
Taq DNA Polymerase
EK2500-01
EURx
200U
EUR 40.33
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs
Taq DNA Polymerase
EK2500-02
EURx
1000U
EUR 163.5
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs
Taq DNA Polymerase
EK2500-03
EURx
5000U
EUR 809.87
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs
Taq DNA Polymerase
EK2500-04
EURx
500U
EUR 90.47
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs
Taq DNA Polymerase
EQ005-100ul500U
ELK Biotech
100μl/500U
EUR 15
Taq DNA Polymerase
EQ005-500ul2500U
ELK Biotech
500μl/2500U
EUR 50
Taq DNA Polymerase
G009
ABM
1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.
Taq DNA Polymerase
L7051001
Biochain
1000 unit
EUR 167
Taq DNA Polymerase
L7051200
Biochain
200 unit
EUR 43
Taq DNA Polymerase
abx299725-100g
Abbexa
100 µg
Ask for price
Taq DNA Polymerase
abx299725-20g
Abbexa
20 µg
EUR 337.5
Taq DNA Polymerase
abx299725-50g
Abbexa
50 µg
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Taq DNA Polymerase
abx461029-1096tests
Abbexa
10 × 96 tests
EUR 1787.5
Taq DNA Polymerase
abx461029-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase
abx461029-96tests
Abbexa
96 tests
EUR 362.5
Taq DNA Polymerase
MB101-0500
Bio-Helix
500 U/100 μl
EUR 10
Taq DNA Polymerase
MBS157418-1000Units
MyBiosource
1000Units
EUR 190
Taq DNA Polymerase
MBS157418-3000Units
MyBiosource
3000Units
EUR 245
Taq DNA Polymerase
MBS157418-500Units
MyBiosource
500Units
EUR 175
Taq DNA Polymerase
MBS157418-5x3000Units
MyBiosource
5x3000Units
EUR 780
Taq DNA Polymerase
MBS402024-500Units
MyBiosource
500Units
EUR 170
Taq DNA Polymerase
MBS402024-50Units
MyBiosource
50Units
EUR 160
T7 RNA Polymerase (50U/ul) (w/10X IVT Buffer)
98343
Sisco Laboratories
1000 Units
EUR 68.42
Description: Part E
T7 RNA Polymerase (50U/ul) (w/10X IVT Buffer)
98343-1
Sisco Laboratories
5000 Units
EUR 333.55
Description: Part E
Taq DNA Polymerase (3000U)
9K-001-0002
Bio Basic
3000U
EUR 582
Taq DNA Polymerase (6000U)
9K-001-0033
Bio Basic
6000U
EUR 1234.5
Taq DNA Polymerase (1000U)
9K-001-0035
Bio Basic
1000U
EUR 418.62
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d1
Vazyme
125 U
EUR 147.6
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d2
Vazyme
500 U
EUR 213.6
Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d1
Vazyme
125 U
EUR 147.6
Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d2
Vazyme
500 U
EUR 213.6
Taq DNA Polymerase (500U)
9K-001-0001
Bio Basic
500U
EUR 209.29
Taq HS DNA Polymerase
P132-d1
Vazyme
500 U
EUR 47.85
Taq HS DNA Polymerase
P132-d2
Vazyme
1,000 U
EUR 89.18
Taq HS DNA Polymerase
P132-d3
Vazyme
5,000 U
EUR 406
Taq HS DNA Polymerase
P132-d1-500U
Vazyme
500 U
EUR 49.55
Taq HS DNA Polymerase
P132-d2-1000U
Vazyme
1,000 U
EUR 92.34
Taq HS DNA Polymerase
P132-d3-5000U
Vazyme
5,000 U
EUR 420.41
HLA Taq DNA Polymerase
E2717-01
EURx
200U
EUR 37.06
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits
HLA Taq DNA Polymerase
E2717-02
EURx
1000U
EUR 146.06
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits
HLA Taq DNA Polymerase
E2717-03
EURx
5000U
EUR 719.4
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits
HLA Taq DNA Polymerase
E2717-04
EURx
500U
EUR 82.84
Description: HLA Taq DNA Polymerase is a specially formulated enzyme for HLA SSP typing Using commerciallyavailable kits
Taq DNA Polymerase (PCR)
G008
ABM
5000 U (1.0 ml)
EUR 246
Taq DNA Polymerase (PCR)
G126
ABM
10000 U (2 x 1.0 ml)
EUR 370.8
Taq DNA Polymerase Mix
abx461043-1096tests
Abbexa
10 × 96 tests
Ask for price
Taq DNA Polymerase Mix
abx461043-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase Mix
abx461043-96tests
Abbexa
96 tests
EUR 337.5
Hot Start Taq Polymerase (5U/mcl)
81646
Sisco Laboratories
250 Units
EUR 66.11
Description: Part E
Hot Start Taq Polymerase (5U/mcl)
81646-1
Sisco Laboratories
1000 Units
EUR 252.56
Description: Part E
Long Taq DNA Polymerase
MBS355971-3000Units
MyBiosource
3000Units
EUR 445
Long Taq DNA Polymerase
MBS355971-500Units
MyBiosource
500Units
EUR 235
Long Taq DNA Polymerase
MBS355971-5x3000Units
MyBiosource
5x3000Units
EUR 1700
Taq DNA polymerase (+dNTPs)
02-001
Sceti
200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (+dNTPs)
02-001-5
Sceti
5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (-dNTPs)
02-011
Sceti
200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (-dNTPs)
02-011-5
Sceti
5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Color Taq DNA Polymerase
E2510-01
EURx
200U
EUR 31.61
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)
Color Taq DNA Polymerase
E2510-02
EURx
1000U
EUR 120.99
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)
Color Taq DNA Polymerase
E2510-03
EURx
5000U
EUR 599.5
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)
Color Taq DNA Polymerase
E2510-04
EURx
500U
EUR 69.76
Description: The enzyme is supplemented with two inert gel tracking dyes (red and yellow),allowing direct loading of PCR products to agarose gels (1.0 U/μl)
Color Taq DNA Polymerase
EK2510-01
EURx
200U
EUR 40.33
Description: Color Taq DNA Polymerase Kit containing polymerase and dNTPs
In order to develop a test to identify the genes responsible for antibiotic resistance in gram-negative bacteria, it has been found that the standard of Taq DNA polymerase (not DNA-free) blaTEM contaminated with fragments of genes that varied in length and number. BlaTEM complete gene sequences are absent or detected in very small amounts. We developed an approach to avoid false-positive findings caused by contamination polymerase gene sequence in a conventional blaTEM