Polymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleic acids DNA or RNA strand using the base-pairing interactions. One of the polymerase enzyme, Taq DNA polymerase, originally isolated from Thermus aquaticus (Taq) is an enzyme widely used in molecular biology so far. The properties of thermostable enzymes has contributed majorly to specificity, automation, and efficacy of the polymerase chain reaction (PCR), making it a powerful tool for molecular biology research today throughout the world.

Purification of Taq DNA polymerase from the original host results in low yields, labor and time consumption. Therefore, much research has been done before to get this enzyme using an alternative host. So far, all of the existing methodology is more laborious, time consuming and requires a heavy cost. We use a new approach to purifying the enzyme with relatively high efficiency, yield and minimum time consumption using Escherichia coli (E. coli) as an alternative host. We cloned 2500 base pairs of DNA Taq polymerase gene into pGEX-4T-1 vector containing GST-tag, downstream of tac promoter and expressed using isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer

The enzyme was purified using chromatography efficient new approach and use in routine PCR tests in our laboratory. Our findings suggest a new approach to facilitate the availability of polymerases for molecular and diagnostic studies. In the future, may be used for purification of other recombinant peptides or proteins used in structural biology and proteomics research-based.

Outside Chelation: EDTA binds Closely Taq DNA Polymerase, Mutt and dUTPase and direct Inhibiting Activity dNTPase.

EDTA is commonly used as a metal ion chelator efficient enzyme cofactor. It is highly soluble, optically inactive and do not interfere with most chemicals used in making standard buffer EDTA common options for generating metal-free conditions for biochemical and biophysical investigations. However, controversy in the literature about the activity of the metal-free enzyme is achieved by using EDTA or otherwise called our attention to the alleged effects of external EDTA chelation. Here, we show that EDTA compete for sites of hydrolases dUTPase nucleotide nucleotide binding by developing a network of interactions within the active site similar to the substrate. To reach these findings, we apply the kinetics and molecular docking technique uses two different dUTPases.

Furthermore, we directly measured the binding of EDTA to dUTPases and two other dNTPases, Taq polymerase and Mutt using isothermal titration calorimetry. EDTA binds proven exothermic and enthalpy driven primarily by submicromolar dissociation constant is much lower than with an enzyme: substrate or Mg: EDTA complex. Control of protein, including ATPase, do not interact with EDTA. Our findings indicate that EDTA can act as a selective inhibitor of the enzyme hydrolysis of dNTPs and urges a rethinking of the use of EDTA in enzymatic trial.

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ200	Allele Biotech	200U	Ask for price

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ500	Allele Biotech	500U	Ask for price

3G HotStart Taq DNA Polymerase (5u/ul)
3GHST81	Bio Basic	5x500U	EUR 746.87

Taq DNA Polymerase (High Purity), 5u/ul
HTD0078	Bio Basic	5X200U	EUR 108

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-01	Vazyme	1000 U	EUR 121

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-02	Vazyme	5000 U	EUR 193

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-03	Vazyme	10000 U	EUR 262

Taq DNA Polymerase (Mg2+ free Buffer)
P102-01	Vazyme	1000 U	EUR 121

Taq DNA Polymerase (Mg2+ free Buffer)
P102-02	Vazyme	5000 U	EUR 193

Taq DNA Polymerase (Mg2+ free Buffer)
P102-03	Vazyme	10000 U	EUR 262

Taq DNA Polymerase
9001-2500	Biovision		EUR 512

Taq DNA Polymerase
9001-500	Biovision		EUR 191

Taq DNA Polymerase
L7051001	Biochain	1000 unit	EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase
L7051200	Biochain	200 unit	EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase
BA00103	Cusabio	500U	EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00104	Bioatlas	1000U	EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00105	Bioatlas	2500U	EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10101	Bioatlas	500U	EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10102	Bioatlas	1000U	EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10103	Bioatlas	2000U	EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

6X DNA Loading Buffer (Blue)
99962-1	Biotium	4x1.5ML	EUR 104
Description: Minimum order quantity: 1 unit of 4x1.5ML

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d1	Vazyme	1000 U	EUR 136

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d2	Vazyme	5000 U	EUR 267

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d3	Vazyme	10000 U	EUR 409

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d1	Vazyme	1000 U	EUR 136

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d2	Vazyme	5000 U	EUR 267

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d3	Vazyme	10000 U	EUR 409

T4 DNA Polymerase, 5u/ul
BEP0061	Bio Basic	100U	EUR 128.31

Pfu DNA Polymerase, 5u/ul
B0093(D0090P)	Bio Basic	500U	EUR 137

Tsg DNA Polymerase, 5u/ul
D0081	Bio Basic	5X200U	EUR 93.5

Taq DNA Polymerase (PCR)
G008	ABM	5000 U (1.0 ml)	EUR 205

Taq DNA Polymerase (PCR)
G009	ABM	1000 U (200 ul)	EUR 87

Taq DNA Polymerase (PCR)
G126	ABM	10000 U (2 x 1.0 ml)	EUR 309

Champagne Taq DNA Polymerase
P122-d1	Vazyme	500 U (2.5 U/μl)	EUR 177

Champagne Taq DNA Polymerase
P122-d2	Vazyme	500 U (5 U/μl)	EUR 177

Champagne Taq DNA Polymerase
P122-d3	Vazyme	500 U (10 U/μl)	EUR 177

Taq Plus DNA Polymerase
P201-01	Vazyme	250 U	EUR 123

Taq Plus DNA Polymerase
P201-02	Vazyme	1000 U	EUR 178

Taq Plus DNA Polymerase
P201-03	Vazyme	3000 U	EUR 319

Hotstart Taq DNA Polymerase
L7052001	Biochain	1000 units	EUR 568
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase
L7052250	Biochain	250 units	EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase (500U)
9K-001-0001	Bio Basic	500U	EUR 174.41

Taq DNA Polymerase (3000U)
9K-001-0002	Bio Basic	3000U	EUR 485

Taq DNA Polymerase (6000U)
9K-001-0033	Bio Basic	6000U	EUR 1028.75

Taq DNA Polymerase (1000U)
9K-001-0035	Bio Basic	1000U	EUR 348.85

Taq DNA Polymerase (Recombinant)
abx073521-10000U	Abbexa	10000 U	EUR 592

Taq DNA Polymerase (Recombinant)
abx073521-1000U	Abbexa	1000 U	EUR 230

Taq DNA Polymerase (Recombinant)
abx073521-3000U	Abbexa	3000 U	EUR 328

Recombinant Taq DNA Polymerase
7-03634	CHI Scientific	1,000U	Ask for price

Recombinant Taq DNA Polymerase
7-03635	CHI Scientific	3,000U	Ask for price

Recombinant Taq DNA Polymerase
7-03636	CHI Scientific	10,000U	Ask for price

Taq DNA polymerase (+dNTPs)
02-001	Sceti	200U	EUR 153
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)
02-001-5	Sceti	5×200U	EUR 257
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)
02-011	Sceti	200U	EUR 140
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)
02-011-5	Sceti	5×200U	EUR 218
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase
S101	GeneOn	500 units	EUR 46

Maximo Taq DNA Polymerase
S102	GeneOn	5x500 units	EUR 107

Maximo Taq DNA Polymerase
S103	GeneOn	20x500 units	EUR 328

Taq DNA Polymerase, 5units/㎕
T2200-050	GenDepot	500units	EUR 131

Taq DNA Polymerase, 5units/㎕
T2200-100	GenDepot	2x500 units	EUR 192

Taq DNA Polymerase, 5units/㎕
T2200-200	GenDepot	4x500 units	EUR 313

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S111	GeneOn	500 units	EUR 53

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S112	GeneOn	5x500 units	EUR 124

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S128	GeneOn	20x500 units	EUR 406

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S129	GeneOn	100x500 units or as bulk	EUR 1785

Taq Plus DNA Polymerase (PCR)
G012	ABM	250 U (50 ul)	EUR 73

Taq Plus DNA Polymerase (PCR)
G040	ABM	1000 U (200 ul)	EUR 106

Taq DNA Polymerase Polyclonal Antibody
7018-100	Biovision		EUR 354

Taq DNA Polymerase Polyclonal Antibody
7018-30T	Biovision		EUR 146

Fast-Taq DNA Polymerase (500U)
9K-001-0036	Bio Basic	500U	EUR 350.54

Taq Plus DNA Polymerase (Recombinant)
abx073522-2500U	Abbexa	2.500 U	EUR 592

Taq Plus DNA Polymerase (Recombinant)
abx073522-250U	Abbexa	250 U	EUR 230

Taq Plus DNA Polymerase (Recombinant)
abx073522-750U	Abbexa	750 U	EUR 328

Taq DNA Polymerase - 5,000 units
3245	Intact Genomics	1/EA	EUR 517

Recombinant Taq Plus DNA Polymerase
7-03631	CHI Scientific	250U	Ask for price

Recombinant Taq Plus DNA Polymerase
7-03632	CHI Scientific	750U	Ask for price

Recombinant Taq Plus DNA Polymerase
7-03633	CHI Scientific	2,500U	Ask for price

YEA taq DNA Polymerase wiodNTP
FYT011-500U	Yeastern Biotech	500U	Ask for price

ACTaq? Taq DNA Polymerase, 1000U
E2100-1000U	ACTGene		EUR 217

ACTaq? Taq DNA Polymerase, 250U
E2100-250U	ACTGene		EUR 102

Anti-Taq DNA Polymerase Antibody
48369-100ul	SAB	100ul	EUR 333

Anti-Taq DNA Polymerase Antibody
48369-50ul	SAB	50ul	EUR 239

Recombinant (E.Coli) Taq DNA Polymerase
RP-394	Alpha Diagnostics	1000 IU	EUR 164

6X Loading Buffer
L0501-005	GenDepot	5X1ml	EUR 77

6X Loading Buffer
L0502-005	GenDepot	5X1ml	EUR 77

amfiXpand Taq DNA Ploymerase Buffer*(2X)
P0332-001	GenDepot	1ml	EUR 101

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B	Alpha Diagnostics	Custom	Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U	Alpha Diagnostics	Custom	Ask for price

Cheetah Hot Start Taq DNA Polymerase
29050	Biotium	500EU	EUR 296
Description: Minimum order quantity: 1 unit of 500EU

YEA taq DNA Polymerase wi dNTP
FYT001-500U	Yeastern Biotech	500U	Ask for price

amfiSure Hot-Start Taq DNA Polymerase
P0322-010	GenDepot	100 units	EUR 147

amfiSure Hot-Start Taq DNA Polymerase
P0322-025	GenDepot	250 units	EUR 228

ACTaq? Taq Blue DNA Polymerase, 1000U
E2100B-1000U	ACTGene		EUR 227

ACTaq? Taq Blue DNA Polymerase, 250U
E2100B-250U	ACTGene		EUR 105

Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B01	GenDepot	10,000 units	EUR 1157

Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B05	GenDepot	5x10,000 units	EUR 5450

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-050	GenDepot	500units	EUR 131

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-100	GenDepot	2x500 units	EUR 192

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-200	GenDepot	4x500 units	EUR 313

Anti-Taq DNA Polymerase Conjugated Antibody
C48369	SAB	100ul	EUR 397

Recombinant (E.Coli) Taq Plus DNA Polymerase
RP-395	Alpha Diagnostics	250 U	EUR 164

6x Protein Loading Buffer
abx098124-5ml	Abbexa	5 ml	EUR 189

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N140	GeneOn	500 units	EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N142	GeneOn	5x500 units	EUR 120

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N144	GeneOn	20x500 units	EUR 367

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled
AD-146-F	Alpha Diagnostics	Custom	Ask for price

10x Taq Buffer
BA00151	Bioatlas	1.8ml	EUR 55
Description: High purity buffer for various PCR applications.

Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018	Bio Basic	6000U	EUR 1280.62

Taq DNA Polymerase with dNTP Mix (500U)
9K-001-0031	Bio Basic	500U	EUR 292.73

Taq DNA Polymerase with dNTP Mix (3000U)
9K-001-0032	Bio Basic	3000U	EUR 907.39

Taq DNA Polymerase with dNTP Mix (1000U)
9K-001-0034	Bio Basic	1000U	EUR 432.37

Taq DNA Polymerase 2x Premix - 500 Reactions
3249	Intact Genomics	1/EA	EUR 190

Hot Start Taq DNA Polymerase - 1000 units
3293	Intact Genomics	1/EA	EUR 310

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-010	GenDepot	100 units	EUR 150

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3
P0330-020	GenDepot	2x100 units	EUR 239

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-050	GenDepot	5X100 units	EUR 388

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-250	GenDepot	25X100 units	EUR 1670

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2
P0330-500	GenDepot	50X100 units	EUR 2993

DNA Loading (6X) Buffer for Agarose and Acrylamide Gels
306-205	GeneOn	5x1 ml	EUR 47

Accuris Taq Polymerase
PR1000-1000	BenchMark	1 PC	EUR 172.66

Accuris Taq Polymerase
PR1000-500	BenchMark	1 PC	EUR 132.79

Accuris Taq Polymerase
PR1000-6000	BenchMark	1 PC	EUR 618.47

Accuris Taq Polymerase
PR1000-S	BenchMark	1 PC	EUR 69.79

Tsg PlusDNA Polymerase, 5u/ul
D0088	Bio Basic	200U	EUR 79

10x Taq Buffer (MgCl2)
PCRB16	Bio Basic	4x1.5ml, 6ml	EUR 58.7

Reversible 1-min Hot Start Taq DNA Polymerase
P950	101Bio	-	Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase
P950L	101Bio	-	Ask for price

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-001	GenDepot	1000 units	EUR 165

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-005	GenDepot	5000 units	EUR 300

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-010	GenDepot	10,000 units	EUR 583

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-100	GenDepot	100,000 units	EUR 2885

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B01	GenDepot	10,000 units	EUR 1157

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B05	GenDepot	5x10,000 units	EUR 5450

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-01	Vazyme	125 U	EUR 121

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-02	Vazyme	500 U	EUR 175

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150	GeneOn	500 units	EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152	GeneOn	5x500 units	EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154	GeneOn	20x500 units	EUR 1525

SDS-PAGE Sample Loading Buffer (6x)
abx090649-5ml	Abbexa	5 ml	EUR 203

1.2ML Triple Dye Loading Buffer (6X)
NAT1192	National Diagnostics	1.2ML	EUR 71

T4 DNA Ligase, 5u/ul
B1122	Bio Basic	1KU	EUR 158.75

T4 DNA Ligase, 5u/ul
B1125	Bio Basic	200U	EUR 77.84

EZ Taq Polymerase (HotStart)
9K-005-0020	Bio Basic	500U	EUR 204.86

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)
9K-001-0003	Bio Basic	500U	EUR 479.35

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)
9K-001-0004	Bio Basic	2500U	EUR 2054.48

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)
9K-001-0005	Bio Basic	250U	EUR 320.14

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)
9K-001-0006	Bio Basic	4x250U	EUR 879.98

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)
9K-001-0020	Bio Basic	5000U	EUR 2399

Taq DNA Polymerase - 5,000 units with separate dNTP Mix
3245d	Intact Genomics	1/EA	EUR 669

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-025	GenDepot	250 unit	EUR 167

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-050	GenDepot	2X250 unit	EUR 250

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-100	GenDepot	4X250 Unit	EUR 421

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-250	GenDepot	10X250 Unit	EUR 739

amfiEco Taq Reaction Buffer(2X)
P0703-001	GenDepot	1ml	EUR 84

amfiEco Taq Reaction Buffer(2X)
P0703-005	GenDepot	5ml	EUR 178

10x Taq Buffer (NH4)2SO4
PCRB55	Bio Basic	4x1.5ml, 6ml	EUR 58.7

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-050	GenDepot	500 units	EUR 165

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-102	GenDepot	25x500 units	EUR 1427

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-500	GenDepot	10X500 units	EUR 739

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S113	GeneOn	2x100 rcs	EUR 66

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S114	GeneOn	10x100 rcs	EUR 193

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S400	GeneOn	200 units	EUR 72

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S410	GeneOn	1000 units	EUR 193

DNA Polymerase
ABD3082	Lifescience Market	100 ug	EUR 438

DNA Polymerase
ABF5235	Lifescience Market	100 ug	EUR 438

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d1	Vazyme	125 U	EUR 123

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d2	Vazyme	500 U	EUR 178

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d1	Vazyme	125 U	EUR 123

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d2	Vazyme	500 U	EUR 178

DNA Loading Buffer
abx098143-5ml	Abbexa	5 ml	EUR 175

DNA Precipitation Buffer
D106	Cygnus Technologies	55 ml	EUR 175
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

utiDNA Polymerase w/ dNTPs
FYT007-500U	Yeastern Biotech	500 Units	Ask for price

6X GelRed Prestain Buffer with Blue Tracking Dyes
41009	Biotium	1mL	EUR 134
Description: Minimum order quantity: 1 unit of 1mL

6X GelRed Prestain Buffer with Orange Tracking Dye
41010	Biotium	1mL	EUR 134
Description: Minimum order quantity: 1 unit of 1mL

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix
3293d	Intact Genomics	1/EA	EUR 357

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-500	GenDepot	500 units	EUR 219

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-502	GenDepot	2x500 units	EUR 354

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-510	GenDepot	10x500 units	EUR 1373

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-525	GenDepot	25x500 units	EUR 3020

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-550	GenDepot	50x500 units	EUR 5383

Fast DNA Ligation Kit (5u/ul, T4)
BS511	Bio Basic	10prep	EUR 70.88

Fast DNA Ligation Kit (5u/ul, T4)
BS512	Bio Basic	50prep	EUR 132.65

T4 DNA Ligase with PEG, 5u/ul
B1442	Bio Basic	1KU	EUR 163.1

T4 DNA Ligase with PEG, 5u/ul
B1445	Bio Basic	200U	EUR 80.45

Taq DNA Ligase - 10,000 units
3219	Intact Genomics	1/EA	EUR 253

PFU DNA Polymerase
9003-2500	Biovision		EUR 512

PFU DNA Polymerase
9003-500	Biovision		EUR 180

Laq? DNA Polymerase
9004-2500	Biovision		EUR 610

Laq? DNA Polymerase
9004-500	Biovision		EUR 218

Kodaq DNA Polymerase
G498	ABM	250 U (50 ul)	EUR 87

In order to develop a test to identify the genes responsible for antibiotic resistance in gram-negative bacteria, it has been found that the standard of Taq DNA polymerase (not DNA-free) blaTEM contaminated with fragments of genes that varied in length and number. BlaTEM complete gene sequences are absent or detected in very small amounts. We developed an approach to avoid false-positive findings caused by contamination polymerase gene sequence in a conventional blaTEM