A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.

Polymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleic acids DNA or RNA strand using the base-pairing interactions. One of the polymerase enzyme, Taq DNA polymerase, originally isolated from Thermus aquaticus (Taq) is an enzyme widely used in molecular biology so far. The properties of thermostable enzymes has contributed majorly to specificity, automation, and efficacy of the polymerase chain reaction (PCR), making it a powerful tool for molecular biology research today throughout the world.

Purification of Taq DNA polymerase from the original host results in low yields, labor and time consumption. Therefore, much research has been done before to get this enzyme using an alternative host. So far, all of the existing methodology is more laborious, time consuming and requires a heavy cost. We use a new approach to purifying the enzyme with relatively high efficiency, yield and minimum time consumption using Escherichia coli (E. coli) as an alternative host. We cloned 2500 base pairs of DNA Taq polymerase gene into pGEX-4T-1 vector containing GST-tag, downstream of tac promoter and expressed using isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer

The enzyme was purified using chromatography efficient new approach and use in routine PCR tests in our laboratory. Our findings suggest a new approach to facilitate the availability of polymerases for molecular and diagnostic studies. In the future, may be used for purification of other recombinant peptides or proteins used in structural biology and proteomics research-based.

 A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.

Outside Chelation: EDTA binds Closely Taq DNA Polymerase, Mutt and dUTPase and direct Inhibiting Activity dNTPase.

EDTA is commonly used as a metal ion chelator efficient enzyme cofactor. It is highly soluble, optically inactive and do not interfere with most chemicals used in making standard buffer EDTA common options for generating metal-free conditions for biochemical and biophysical investigations. However, controversy in the literature about the activity of the metal-free enzyme is achieved by using EDTA or otherwise called our attention to the alleged effects of external EDTA chelation. Here, we show that EDTA compete for sites of hydrolases dUTPase nucleotide nucleotide binding by developing a network of interactions within the active site similar to the substrate. To reach these findings, we apply the kinetics and molecular docking technique uses two different dUTPases.

Furthermore, we directly measured the binding of EDTA to dUTPases and two other dNTPases, Taq polymerase and Mutt using isothermal titration calorimetry. EDTA binds proven exothermic and enthalpy driven primarily by submicromolar dissociation constant is much lower than with an enzyme: substrate or Mg: EDTA complex. Control of protein, including ATPase, do not interact with EDTA. Our findings indicate that EDTA can act as a selective inhibitor of the enzyme hydrolysis of dNTPs and urges a rethinking of the use of EDTA in enzymatic trial.

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price
    • Product line: PCR Reagents
    • Brand:

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price
    • Product line: PCR Reagents
    • Brand:

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price
    • Product line: PCR Reagents
    • Brand:

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price
    • Product line: PCR Reagents
    • Brand:

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 746.87
  • Product category: PCR Related/Enzymes/DNA Polymerases

Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 108
  • Product category: PCR Related/Enzymes/DNA Polymerases

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 262

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 262

Taq DNA Polymerase

9001-2500
EUR 512

Taq DNA Polymerase

9001-500
EUR 191

Taq DNA polymerase

BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

L7051001 1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

6X DNA Loading Buffer (Blue)

99962-1 4x1.5ML
EUR 104
Description: Minimum order quantity: 1 unit of 4x1.5ML

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 409

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 409

Pfu DNA Polymerase, 5u/ul

B0093(D0090P) 500U
EUR 137
  • Product category: PCR Related/Enzymes/DNA Polymerases

T4 DNA Polymerase, 5u/ul

BEP0061 100U
EUR 128.31
  • Product category: PCR Related/Enzymes/DNA Polymerases

Tsg DNA Polymerase, 5u/ul

D0081 5X200U
EUR 93.5
  • Product category: PCR Related/Enzymes/DNA Polymerases

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 153
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 257
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 140
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 218
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 592
  • Shipped within 5-10 working days.

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 230
  • Shipped within 5-10 working days.

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 328
  • Shipped within 5-10 working days.

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 205

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 87

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 309

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 177

Taq Plus DNA Polymerase

P201-01 250 U
EUR 123

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 178

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 319

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 568
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Maximo Taq DNA Polymerase

S101 500 units
EUR 46

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 107

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 328

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 131

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 192

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 313

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 174.41
  • Product category: PCR Related/Enzymes/DNA Polymerases

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 485
  • Product category: PCR Related/Enzymes/DNA Polymerases

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1028.75
  • Product category: PCR Related/Enzymes/DNA Polymerases

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 348.85
  • Product category: PCR Related/Enzymes/DNA Polymerases

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S111 500 units
EUR 53

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S112 5x500 units
EUR 124

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S128 20x500 units
EUR 406

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S129 100x500 units or as bulk
EUR 1785

6X Loading Buffer

L0501-005 5X1ml
EUR 77

6X Loading Buffer

L0502-005 5X1ml
EUR 77

Taq DNA Polymerase Polyclonal Antibody

7018-100
EUR 354

Taq DNA Polymerase Polyclonal Antibody

7018-30T
EUR 146

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 333

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 239

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 517

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 592
  • Shipped within 5-10 working days.

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 230
  • Shipped within 5-10 working days.

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 328
  • Shipped within 5-10 working days.

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U
EUR 217

ACTaq? Taq DNA Polymerase, 250U

E2100-250U
EUR 102

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Taq Plus DNA Polymerase (PCR)

G012 250 U (50 ul)
EUR 73

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
EUR 106

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 164

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 350.54
  • Product category: PCR Related/Enzymes/DNA Polymerases

amfiXpand Taq DNA Ploymerase Buffer*(2X)

P0332-001 1ml
EUR 101

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 296
Description: Minimum order quantity: 1 unit of 500EU

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U
EUR 227

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U
EUR 105

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 397

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 147

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 228

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 164

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1157

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 5450

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 131

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-100 2x500 units
EUR 192

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-200 4x500 units
EUR 313

6x Protein Loading Buffer

abx098124-5ml 5 ml
EUR 189
  • Shipped within 5-10 working days.

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 120

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 367

10x Taq Buffer

BA00151 1.8ml
EUR 55
Description: High purity buffer for various PCR applications.

DNA Loading (6X) Buffer for Agarose and Acrylamide Gels

306-205 5x1 ml
EUR 47

Hot Start Taq DNA Polymerase - 1000 units

3293 1/EA
EUR 310

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 190

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-010 100 units
EUR 150

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3

P0330-020 2x100 units
EUR 239

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-050 5X100 units
EUR 388

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-250 25X100 units
EUR 1670

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-500 50X100 units
EUR 2993

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1280.62
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 292.73
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 907.39
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 432.37
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Accuris Taq Polymerase

PR1000-1000 1 PC
EUR 172.66
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Polymerase

PR1000-500 1 PC
EUR 132.79
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Polymerase

PR1000-6000 1 PC
EUR 618.47
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Polymerase

PR1000-S 1 PC
EUR 69.79
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

10x Taq Buffer (MgCl2)

PCRB16 4x1.5ml, 6ml
EUR 58.7
  • Product category: PCR Related/PCR Buffers

Reversible 1-min Hot Start Taq DNA Polymerase

P950 NULL
EUR 0

Reversible 1-min Hot Start Taq DNA Polymerase

P950L NULL
EUR 0

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 165

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 300

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 583

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-100 100,000 units
EUR 2885

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B01 10,000 units
EUR 1157

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B05 5x10,000 units
EUR 5450

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-01 125 U
EUR 121

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-02 500 U
EUR 175

Tsg PlusDNA Polymerase, 5u/ul

D0088 200U
EUR 79
  • Product category: PCR Related/Enzymes/DNA Polymerases

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1525

SDS-PAGE Sample Loading Buffer (6x)

abx090649-5ml 5 ml
EUR 203
  • Shipped within 5-10 working days.

1.2ML Triple Dye Loading Buffer (6X)

NAT1192 1.2ML
EUR 71

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 669

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-025 250 unit
EUR 167

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-050 2X250 unit
EUR 250

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-100 4X250 Unit
EUR 421

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-250 10X250 Unit
EUR 739

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 479.35
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2054.48
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 320.14
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 879.98
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2399
  • Product category: PCR Related/dNTPs + Polymerase/dNTP Mix + Taq

EZ Taq Polymerase (HotStart)

9K-005-0020 500U
EUR 204.86
  • Product category: PCR Related/Enzymes/DNA Polymerases

T4 DNA Ligase, 5u/ul

B1122 1KU
EUR 158.75
  • Product category: Proteins/Enzymes/DNA Ligases

T4 DNA Ligase, 5u/ul

B1125 200U
EUR 77.84
  • Product category: Proteins/Enzymes/DNA Ligases

amfiEco Taq Reaction Buffer(2X)

P0703-001 1ml
EUR 84

amfiEco Taq Reaction Buffer(2X)

P0703-005 5ml
EUR 178

10x Taq Buffer (NH4)2SO4

PCRB55 4x1.5ml, 6ml
EUR 58.7
  • Product category: PCR Related/PCR Buffers

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-050 500 units
EUR 165

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-102 25x500 units
EUR 1427

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-500 10X500 units
EUR 739

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S113 2x100 rcs
EUR 66

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S114 10x100 rcs
EUR 193

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 72

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 193

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d1 125 U
EUR 123

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d2 500 U
EUR 178

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d1 125 U
EUR 123

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d2 500 U
EUR 178

DNA Polymerase

ABD3082 100 ug
EUR 438

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Precipitation Buffer

D106 55 ml
EUR 175
  • Product category: DNA and RNA extraction and purification
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

DNA Loading Buffer

abx098143-5ml 5 ml
EUR 175
  • Shipped within 5-10 working days.

6X GelRed Prestain Buffer with Blue Tracking Dyes

41009 1mL
EUR 134
Description: Minimum order quantity: 1 unit of 1mL

utiDNA Polymerase w/ dNTPs

FYT007-500U 500 Units Ask for price

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 357

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-500 500 units
EUR 219

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-502 2x500 units
EUR 354

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-510 10x500 units
EUR 1373

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-525 25x500 units
EUR 3020

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-550 50x500 units
EUR 5383

Taq DNA Ligase - 10,000 units

3219 1/EA
EUR 253

GelRed Prestain Plus 6X DNA Loading Dye

41011 1mL
EUR 147
Description: Minimum order quantity: 1 unit of 1mL

T4 DNA Ligase with PEG, 5u/ul

B1442 1KU
EUR 163.1
  • Product category: Proteins/Enzymes/DNA Ligases

T4 DNA Ligase with PEG, 5u/ul

B1445 200U
EUR 80.45
  • Product category: Proteins/Enzymes/DNA Ligases

Fast DNA Ligation Kit (5u/ul, T4)

BS511 10prep
EUR 70.88
  • Product category: Molecular Biology Kits/Blunting/Ligation

Fast DNA Ligation Kit (5u/ul, T4)

BS512 50prep
EUR 132.65
  • Product category: Molecular Biology Kits/Blunting/Ligation

DNA polymerase ? (Rat)

10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

PFU DNA Polymerase

9003-2500
EUR 512


In order to develop a test to identify the genes responsible for antibiotic resistance in gram-negative bacteria, it has been found that the standard of Taq DNA polymerase (not DNA-free) blaTEM contaminated with fragments of genes that varied in length and number. BlaTEM complete gene sequences are absent or detected in very small amounts. We developed an approach to avoid false-positive findings caused by contamination polymerase gene sequence in a conventional blaTEM