Polymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleic acids DNA or RNA strand using the base-pairing interactions. One of the polymerase enzyme, Taq DNA polymerase, originally isolated from Thermus aquaticus (Taq) is an enzyme widely used in molecular biology so far. The properties of thermostable enzymes has contributed majorly to specificity, automation, and efficacy of the polymerase chain reaction (PCR), making it a powerful tool for molecular biology research today throughout the world.
Purification of Taq DNA polymerase from the original host results in low yields, labor and time consumption. Therefore, much research has been done before to get this enzyme using an alternative host. So far, all of the existing methodology is more laborious, time consuming and requires a heavy cost. We use a new approach to purifying the enzyme with relatively high efficiency, yield an d minimum time consumption using Escherichia coli (E. coli) as an alternative host. We cloned 2500 base pairs of DNA Taq polymerase gene into pGEX-4T-1 vector containing GST-tag, downstream of tac promoter and expressed using isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer
The enzyme was purified using chromatography efficient new approach and use in routine PCR tests in our laboratory. Our findings suggest a new approach to facilitate the availability of polymerases for molecular and diagnostic studies. In the future, may be used for purification of other recombinant peptides or proteins used in structural biology and proteomics research-based.
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.
Outside Chelation: EDTA binds Closely Taq DNA Polymerase, Mutt and dUTPase and direct Inhibiting Activity dNTPase.
EDTA is commonly used as a metal ion chelator efficient enzyme cofactor. It is highly soluble, optically inactive and do not interfere with most chemicals used in making standard buffer EDTA common options for generating metal-free conditions for biochemical and biophysical investigations. However, controversy in the literature about the activity of the metal-free enzyme is achieved by using EDTA or otherwise called our attention to the alleged effects of external EDTA chelation. Here, we show that EDTA compete for sites of hydrolases dUTPase nucleotide nucleotide binding by developing a network of interactions within the active site similar to the substrate. To reach these findings, we apply the kinetics and molecular docking technique uses two different dUTPases.
Furthermore, we directly measured the binding of EDTA to dUTPases and two other dNTPases, Taq polymerase and Mutt using isothermal titration calorimetry. EDTA binds proven exothermic and enthalpy driven primarily by submicromolar dissociation constant is much lower than with an enzyme: substrate or Mg: EDTA complex. Control of protein, including ATPase, do not interact with EDTA. Our findings indicate that EDTA can act as a selective inhibitor of the enzyme hydrolysis of dNTPs and urges a rethinking of the use of EDTA in enzymatic trial.
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-02
Vazyme
5000 U
EUR 231.6
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-03
Vazyme
10000 U
EUR 290
Taq DNA Polymerase (Mg2+ free Buffer)
P102-01
Vazyme
1000 U
EUR 145.2
Taq DNA Polymerase (Mg2+ free Buffer)
P102-02
Vazyme
5000 U
EUR 231.6
Taq DNA Polymerase (Mg2+ free Buffer)
P102-03
Vazyme
10000 U
EUR 314.4
Taq DNA Polymerase with dNTP Mix Buffer
11140015-1
Bio-WORLD
500 Unit (s)
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Taq DNA Polymerase with dNTP Mix Buffer
11140015-2
Bio-WORLD
2500 Unit (s)
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Taq DNA Polymerase with dNTP Mix Buffer
11140015-3
Bio-WORLD
5000 Unit (s)
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HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)
RK20601
Abclonal
25 U
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Taq Polymerase (5U/µl)
MBT060A-1000U
EWC Diagnostics
1 unit
EUR 62.03
Description: Taq Polymerase (5U/µl)
Taq Polymerase (5U/µl)
MBT060A-5000U
EWC Diagnostics
1 unit
EUR 241.01
Description: Taq Polymerase (5U/µl)
Taq Polymerase (5U/µl)
MBT060A-500U
EWC Diagnostics
1 unit
EUR 41.58
Description: Taq Polymerase (5U/µl)
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d1
Vazyme
1000 U
EUR 163.2
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d2
Vazyme
5000 U
EUR 320.4
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d3
Vazyme
10000 U
EUR 490.8
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d1
Vazyme
1000 U
EUR 163.2
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d2
Vazyme
5000 U
EUR 320.4
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d3
Vazyme
10000 U
EUR 490.8
Taq DNA Polymerase, 5u/ul
B0089(D0089)
Bio Basic
5X200U
EUR 112.2
Taq Polymerase / Labeling Buffer
PCR-201L
Jena Bioscience GmbH
5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli
Taq Polymerase / Labeling Buffer
PCR-201S
Jena Bioscience GmbH
200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli
HotStart Taq DNA Polymerase (5U/uL)
A4144-250U
Biomatik Corporation
250U
EUR 66
Description: Biotechnology
Taq Plus DNA Polymerase, 5u/ul
D0090
Bio Basic
200U
EUR 94.8
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-1
Glycomatrix
500 Unit(s)
EUR 108.39
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-2
Glycomatrix
2500 Unit(s)
EUR 490.36
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-3
Glycomatrix
5000 Unit(s)
EUR 912.79
Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ001
Allele Biotech
1000U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ100
Allele Biotech
100U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ200
Allele Biotech
200U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ500
Allele Biotech
500U
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3G HotStart Taq DNA Polymerase (5u/ul)
3GHST81
Bio Basic
5x500U
EUR 896.24
Taq DNA Polymerase (High Purity), 5u/ul
HTD0078
Bio Basic
5X200U
EUR 129.6
AtMax Taq DNA Polymerase (2.5U/µl), 200U
PL4201
Vivantis
each
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AtMax Taq DNA Polymerase (2.5U/µl), 500U
PL4202
Vivantis
each
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Chromo Taq DNA Polymerase (1U/µl), 200U
PL1205
Vivantis
each
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Chromo Taq DNA Polymerase (1U/µl), 500U
PL1206
Vivantis
each
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DNA Polymerase Reaction Buffer
abx461036-1096tests
Abbexa
10 × 96 tests
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DNA Polymerase Reaction Buffer
abx461036-596tests
Abbexa
5 × 96 tests
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DNA Polymerase Reaction Buffer
abx461036-96tests
Abbexa
96 tests
EUR 562.5
Pfu DNA Polymerase (5U/µl), 100U
PL5201
Vivantis
each
Ask for price
Pfu DNA Polymerase (5U/µl), 500U
PL5202
Vivantis
each
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AtTaq DNA Polymerase (5U/µl), 200U
PL3201
Vivantis
each
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AtTaq DNA Polymerase (5U/µl), 500U
PL3202
Vivantis
each
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MaxTaq DNA Polymerase (5U/µl), 200U
PL2201
Vivantis
each
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MaxTaq DNA Polymerase (5U/µl), 500U
PL2202
Vivantis
each
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Long-Taq DNA Polymerase (5U/uL)
A4186-250U
Biomatik Corporation
250U
EUR 66
Description: Biotechnology
Taq DNA Polymerase
G009
ABM
1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.
Taq DNA polymerase
BT10101
Bioatlas
500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10102
Bioatlas
1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10103
Bioatlas
2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00103
Cusabio
500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00104
Bioatlas
1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00105
Bioatlas
2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
9001-2500
Biovision
each
EUR 614.4
Taq DNA Polymerase
9001-500
Biovision
each
EUR 229.2
Taq DNA Polymerase
L7051001
Biochain
1000 unit
EUR 167
Taq DNA Polymerase
L7051200
Biochain
200 unit
EUR 43
Taq DNA Polymerase
MB101-0500
Bio-Helix
500 U/100 μl
EUR 10
Taq DNA Polymerase
abx299725-100g
Abbexa
100 µg
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Taq DNA Polymerase
abx299725-20g
Abbexa
20 µg
EUR 337.5
Taq DNA Polymerase
abx299725-50g
Abbexa
50 µg
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Taq DNA Polymerase
abx461029-1096tests
Abbexa
10 × 96 tests
EUR 1787.5
Taq DNA Polymerase
abx461029-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase
abx461029-96tests
Abbexa
96 tests
EUR 362.5
Taq Polymerase(3U/µl)
MBT060B-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060B-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060B-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)
Taq Polymerase(1U/µl)
MBT060D-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060D-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060D-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)
h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl
11140022-1
Bio-WORLD
250 Unit (s)
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h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl
11140022-2
Bio-WORLD
1000 Unit (s)
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Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-01
Vazyme
125 U
EUR 145.2
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-02
Vazyme
500 U
EUR 210
Taq DNA Polymerase (3000U)
9K-001-0002
Bio Basic
3000U
EUR 582
Taq DNA Polymerase (6000U)
9K-001-0033
Bio Basic
6000U
EUR 1234.5
Taq DNA Polymerase (1000U)
9K-001-0035
Bio Basic
1000U
EUR 418.62
Taq DNA Polymerase (500U)
9K-001-0001
Bio Basic
500U
EUR 209.29
Taq HS DNA Polymerase
P132-d1
Vazyme
500 U
EUR 47.85
Taq HS DNA Polymerase
P132-d2
Vazyme
1,000 U
EUR 89.18
Taq HS DNA Polymerase
P132-d3
Vazyme
5,000 U
EUR 406
Taq DNA Polymerase (PCR)
G008
ABM
5000 U (1.0 ml)
EUR 246
Taq DNA Polymerase (PCR)
G126
ABM
10000 U (2 x 1.0 ml)
EUR 370.8
Taq DNA Polymerase Mix
abx461043-1096tests
Abbexa
10 × 96 tests
Ask for price
Taq DNA Polymerase Mix
abx461043-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase Mix
abx461043-96tests
Abbexa
96 tests
EUR 337.5
Taq DNA polymerase (-dNTPs)
02-011
Sceti
200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (-dNTPs)
02-011-5
Sceti
5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (+dNTPs)
02-001
Sceti
200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (+dNTPs)
02-001-5
Sceti
5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA Polymerase, 5units/㎕
T2200-050
GenDepot
500units
EUR 157.2
Taq DNA Polymerase, 5units/㎕
T2200-100
GenDepot
2x500 units
EUR 230.4
Taq DNA Polymerase, 5units/㎕
T2200-200
GenDepot
4x500 units
EUR 375.6
Maximo Taq DNA Polymerase
S101
GeneOn
500 units
EUR 55.2
Maximo Taq DNA Polymerase
S102
GeneOn
5x500 units
EUR 128.4
Maximo Taq DNA Polymerase
S103
GeneOn
20x500 units
EUR 393.6
Hotstart Taq DNA Polymerase
L7052001
Biochain
1000 units
EUR 595
Hotstart Taq DNA Polymerase
L7052250
Biochain
250 units
EUR 167
Taq DNA Polymerase -1000 units
3243
Intact Genomics
1000units
EUR 115
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer
Champagne Taq DNA Polymerase
P122-d1
Vazyme
500 U (2.5 U/μl)
EUR 212.4
Champagne Taq DNA Polymerase
P122-d2
Vazyme
500 U (5 U/μl)
EUR 212.4
Champagne Taq DNA Polymerase
P122-d3
Vazyme
500 U (10 U/μl)
EUR 212.4
ACTaq? Taq DNA Polymerase, 1000U
E2100-1000U
ACTGene
each
EUR 260.4
Taq DNA Polymerase - 5,000 units
3245
Intact Genomics
1/EA
EUR 449
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer
Taq DNA Polymerase (Recombinant)
22060707-1
Glycomatrix
1,000 Unit(s)
EUR 137.27
Taq DNA Polymerase (Recombinant)
22060707-2
Glycomatrix
1500 Unit(s)
EUR 182.1
Taq DNA Polymerase (Recombinant)
22060707-3
Glycomatrix
5000 Unit(s)
EUR 465.77
Taq DNA Polymerase (Recombinant)
22060707-4
Glycomatrix
10 KU
EUR 840.96
Recombinant Taq DNA Polymerase
7-03634
CHI Scientific
1,000U
Ask for price
Recombinant Taq DNA Polymerase
7-03635
CHI Scientific
3,000U
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Recombinant Taq DNA Polymerase
7-03636
CHI Scientific
10,000U
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Taq DNA Polymerase (Recombinant)
abx073521-10000U
Abbexa
10000 U
EUR 710.4
Taq DNA Polymerase (Recombinant)
abx073521-1000U
Abbexa
1000 U
EUR 276
Taq DNA Polymerase (Recombinant)
abx073521-3000U
Abbexa
3000 U
EUR 393.6
Taq DNA Polymerase Recombinant
rAP-1062
Angio Proteomie
Inquiry
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ACTaq? Taq DNA Polymerase, 250U
E2100-250U
ACTGene
each
EUR 122.4
Taq Plus DNA Polymerase
P201-01
Vazyme
250 U
EUR 147.6
Taq Plus DNA Polymerase
P201-02
Vazyme
1000 U
EUR 213.6
Taq Plus DNA Polymerase
P201-03
Vazyme
3000 U
EUR 382.8
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d1
Vazyme
125 U
EUR 147.6
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d2
Vazyme
500 U
EUR 213.6
Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d1
Vazyme
125 U
EUR 147.6
Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d2
Vazyme
500 U
EUR 213.6
Taq Plus DNA Polymerase (Taq Plus DNA) Protein
abx073522-100tests
Abbexa
100 tests
EUR 225
Hot start Taq DNA Polymerase
EQ006
ELK Biotech
100μl/500U
EUR 200
Hot Start Taq DNA Polymerase
abx460010-1096tests
Abbexa
10 × 96 tests
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Hot Start Taq DNA Polymerase
abx460010-596tests
Abbexa
5 × 96 tests
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Hot Start Taq DNA Polymerase
abx460010-96tests
Abbexa
96 tests
EUR 262.5
Taq DNA Polymerase (TaqDNA) Protein
abx073521-100tests
Abbexa
100 tests
EUR 225
Apex Red Taq DNA Polymerase, 10000u
42-402R
Genesee Scientific
20 x 500u/Unit
EUR 1229.49
Description: 10X NH4 Buffer, Mg Free
Apex Red Taq DNA Polymerase, 500u
42-409R
Genesee Scientific
1 x 500u/Unit
EUR 62.1
Description: 10X NH4 Buffer, Mg Free
Taq HS Red DNA Polymerase
abx461041-1096tests
Abbexa
10 × 96 tests
Ask for price
Taq HS Red DNA Polymerase
abx461041-596tests
Abbexa
5 × 96 tests
EUR 2187.5
Taq HS Red DNA Polymerase
abx461041-96tests
Abbexa
96 tests
EUR 1062.5
Taq Plus DNA Polymerase (PCR)
G012
ABM
250 U (50 ul)
EUR 87.6
Taq Plus DNA Polymerase (PCR)
G040
ABM
1000 U (200 ul)
EUR 127.2
Taq DNA Polymerase Polyclonal Antibody
7018-100
Biovision
each
EUR 424.8
Taq DNA Polymerase Polyclonal Antibody
7018-30T
Biovision
each
EUR 175.2
AffiTaq® DNA Polymerase - With Buffer - With MgCl2 - Without dNTP
AFG-BOR-101
AffiGEN
10X (1000U/200µL)
EUR 380
Taq DNA Polymerase with Red Dye
abx461047-1096tests
Abbexa
10 × 96 tests
EUR 1787.5
Taq DNA Polymerase with Red Dye
abx461047-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase with Red Dye
abx461047-96tests
Abbexa
96 tests
EUR 362.5
ACTaq? Taq Blue DNA Polymerase, 1000U
E2100B-1000U
ACTGene
each
EUR 272.4
Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U
Alpha Diagnostics
Custom
Ask for price
ACTaq? Taq Blue DNA Polymerase, 250U
E2100B-250U
ACTGene
each
EUR 126
Taq HS DNA Polymerase (Glycerol-free)
QL101-01
Vazyme
1,000 U
EUR 89
Taq HS DNA Polymerase (Glycerol-free)
QL101-02
Vazyme
5,000 U
EUR 406
Taq HS DNA Polymerase (Glycerol-free)
QL101-03
Vazyme
50,000 U
EUR 3625
Recombinant Taq Plus DNA Polymerase
7-03631
CHI Scientific
250U
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Recombinant Taq Plus DNA Polymerase
7-03632
CHI Scientific
750U
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Recombinant Taq Plus DNA Polymerase
7-03633
CHI Scientific
2,500U
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Taq Plus DNA Polymerase (Recombinant)
abx073522-2500U
Abbexa
2.500 U
EUR 710.4
Taq Plus DNA Polymerase (Recombinant)
abx073522-250U
Abbexa
250 U
EUR 276
Taq Plus DNA Polymerase (Recombinant)
abx073522-750U
Abbexa
750 U
EUR 393.6
Taq Plus DNA Polymerase Recombinant
rAP-1063
Angio Proteomie
Inquiry
Ask for price
Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B01
GenDepot
10,000 units
EUR 1388.4
Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B05
GenDepot
5x10,000 units
EUR 6540
Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B
Alpha Diagnostics
Custom
Ask for price
Taq DNA Polymerase M101 (5,000 U/mL)
RK26000
Abclonal
250U
EUR 60
amfiSure Hot-Start Taq DNA Polymerase
P0322-010
GenDepot
100 units
EUR 176.4
amfiSure Hot-Start Taq DNA Polymerase
P0322-025
GenDepot
250 units
EUR 273.6
T4 DNA Polymerase, 5u/ul
BEP0061
Bio Basic
100U
EUR 153.97
DNA Amplification Kit with Taq DNA Polymerase, 100app
PL1202-K
Vivantis
each
Ask for price
Tsg DNA Polymerase, 5u/ul
D0081
Bio Basic
5X200U
EUR 112.2
Pfu DNA Polymerase, 5u/ul
B0093(D0090P)
Bio Basic
500U
EUR 164.4
Taq DNA Polymerase with Red Dye Mix
abx461048-1096tests
Abbexa
10 × 96 tests
Ask for price
Taq DNA Polymerase with Red Dye Mix
abx461048-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase with Red Dye Mix
abx461048-96tests
Abbexa
96 tests
EUR 325
Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018
Bio Basic
6000U
EUR 1536.74
Taq DNA Polymerase with dNTP Mix (500U)
9K-001-0031
Bio Basic
500U
EUR 351.28
Taq DNA Polymerase with dNTP Mix (3000U)
9K-001-0032
Bio Basic
3000U
EUR 1088.87
Taq DNA Polymerase with dNTP Mix (1000U)
9K-001-0034
Bio Basic
1000U
EUR 518.84
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N140
GeneOn
500 units
EUR 60
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N142
GeneOn
5x500 units
EUR 144
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N144
GeneOn
20x500 units
EUR 440.4
In order to develop a test to identify the genes responsible for antibiotic resistance in gram-negative bacteria, it has been found that the standard of Taq DNA polymerase (not DNA-free) blaTEM contaminated with fragments of genes that varied in length and number. BlaTEM complete gene sequences are absent or detected in very small amounts. We developed an approach to avoid false-positive findings caused by contamination polymerase gene sequence in a conventional blaTEM