Description: Human Sirtuin 7 (GenBank Accession No. NM_016538), full length with C-terminal FLAG-tag, MW= 46 kDa, expressed in an E. coli expression system.

Description: Human recombinant SET domain containing lysine methyltransferase 7 (SETD7), or SET7/9 (GenBank Accession No. NM_030648), a.a. 52-266 (end) with N-terminal His-GST-tag, MW = 63 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Description: Human NCOR2/SMRT, DAD domain, a.a. 395-489, GenBank Accession No. NM_006312, N-terminal GST-tag, MW= 39 kDa, expressed in E. coli system. Source: E. coli. Purity: > 60% by SDS-PAGE.

Description: Human HDAC11 (full length) (GenBank Accession No. NM_024827) untagged, MW= 39.6 kDa, expressed in baculovirus expression system.

Description: The Sirtuin 6 Fluorogenic Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.

Description: HDAC assay developer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included as an 'inhibitor stop' for class I and II HDACs.

Description: HDAC assay buffer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.) in HDAC assay buffer. Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included asan 'inhibitor stop' for class I and II HDACs.

Description: A fluorogenic, acetylated peptide substrate for HDAC2, HDAC3, and HDAC6 (tested so far). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. It should be used in conjunction with HDAC Developer, Cat. # 50030.

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening and_x000D_profiling applications. It comes in a convenient 96-well format, with all the reagents_x000D_necessary for 100 fluorescent HDAC activity measurements. In addition, the kit_x000D_includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,_x000D_for use as a positive and negative control. The Fluorogenic HDAC Assay_x000D_Kit is based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed_x000D_to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing_x000D_an acetylated lysine side chain, is incubated with purified HDAC enzyme._x000D_The deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence reader.

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate

Description: A fluorogenic, acetylated peptide substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC substrate 2 (green), BPS catalog #50038. For class 2a HDACs (HDACs 4, 5, 7, and 9) or HDACs 8 and 11, we recommend #50040.

Description: A fluorogenic, acetylated peptide substrate for HDACs. It is a suitable substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 1/2b HDAC substrate, #50037, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend HDAC substrate 3, #50037. For autofluorescent samples using HDACs 4, 5, 7, 8, 9, and 11, we recommend substrate #50042.

Description: A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7, and 9). It also works well for HDACs 8 and 11. This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC class 2a substrate 2 (green), BPS catalog #50042. For HDACs 1-3 ,6, and 10, we recommend substrate #50037.

Description: A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7 and 9). It also works well for HDACs 8 and 11. This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 2a HDAC substrate, #50040, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend product #50040. For autofluorescent samples using HDACs 1-3 ,6, and 10, we recommend substrate #50038.

Description: Human HDAC5 , GenBank Accession No. NM_005474, full length with N-terminal GST-tag, MW= 150 kDa, expressed in baculovirus expression system.

Description: Human HDAC6 with H216A mutation (GenBank Accession No. NM_006044), full length with N-terminal GST-tag, MW= 161 kDa, expressed in a baculovirus expression system.

Description: Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal FLAG and C-terminal His-tag, MW= 56 kDa, expressed in baculovirus expression system.