Biotium Laboratories manufactures the nucview fluorogenic substrates reagents distributed by Genprice. The Nucview Fluorogenic Substrates reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Biotium. Other Nucview products are available in stock. Specificity: Nucview Category: Fluorogenic Group: Substrates
HDAC Fluorogenic Assay Kit |
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50033 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening and_x000D_profiling applications. It comes in a convenient 96-well format, with all the reagents_x000D_necessary for 100 fluorescent HDAC activity measurements. In addition, the kit_x000D_includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,_x000D_for use as a positive and negative control. The Fluorogenic HDAC Assay_x000D_Kit is based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed_x000D_to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing_x000D_an acetylated lysine side chain, is incubated with purified HDAC enzyme._x000D_The deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence reader. |
Fluorogenic HDAC substrate 3 |
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50037 | BPS Bioscience | 250 nmole | EUR 255 |
Description: A fluorogenic, acetylated peptide substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC substrate 2 (green), BPS catalog #50038. For class 2a HDACs (HDACs 4, 5, 7, and 9) or HDACs 8 and 11, we recommend #50040. |
HDAC1 Fluorogenic Assay Kit |
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50061 | BPS Bioscience | 96 rxns. | EUR 465 |
Description: The Fluorogenic HDAC1 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified_x000D_HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC1. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_ |
HDAC2 Fluorogenic Assay Kit |
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50062 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC2 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 2 (HDAC2) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified_x000D_HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC2 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC2. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader. |
HDAC4 Fluorogenic Assay Kit |
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50064 | BPS Bioscience | 96 rxns. | EUR 465 |
Description: The Fluorogenic HDAC4 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 4 (HDAC4) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC4 activity measurements. In addition, the kit includes purified_x000D_HDAC4 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC4 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC4 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC4. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D__x000D_ |
HDAC5 Fluorogenic Assay Kit |
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50065 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC5 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 5 (HDAC5) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC5 activity measurements. In addition, the kit includes purified_x000D_HDAC5 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC5 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC5 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC5. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_ |
HDAC7 Fluorogenic Assay Kit |
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50067 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC7 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 7 (HDAC7) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC7 activity measurements. In addition, the kit includes purified_x000D_HDAC7 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC7 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC7 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC7. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_ |
HDAC8 Fluorogenic Assay Kit |
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50068 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC 8 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) 8 activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC activity measurements. In addition, the kit includes purified_x000D_HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative_x000D_control. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate_x000D_and developer combination. This assay method eliminates dealing with the radioactivity,_x000D_extraction, and chromatography aspects of traditional assays. Using this kit, only twoa_x000D_simple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First,_x000D_the HDAC fluorometric substrate is incubated with purified HDAC8 enzyme. The_x000D_deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence_x000D_reader. |
HDAC9 Fluorogenic Assay Kit |
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50069 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC9 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 9 (HDAC9) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC9 activity measurements. In addition, the kit includes purified_x000D_HDAC9 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC9 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC9 activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC9. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_ |
HDAC3 Fluorogenic Assay Kit |
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50073 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC3 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 3 (HDAC3) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified_x000D_HDAC3 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC3 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader. |
HDAC6 Fluorogenic Assay Kit |
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50076-1 | BPS Bioscience | 96 rxns. | EUR 565 |
Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_ HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_ substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader. |
HDAC6 Fluorogenic Assay Kit |
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50076-2 | BPS Bioscience | 384 rxns. | EUR 900 |
Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader. |
Fluorogenic Sirtuin 5 Substrate |
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50126 | BPS Bioscience | 250 nmole | EUR 235 |
Description: A fluorogenic, succinylated peptide substrate for Sirtuin 5. This substrate should be used in conjunction with SIRT Developer, #50089. Optimal excitation wavelength 350-380 nm, emission wavelength 440-460 nm. |
HDAC11 Fluorogenic Assay Kit |
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50687 | BPS Bioscience | 96 rxns. | EUR 465 |
Description: The HDAC11 Fluorogenic Assay Kit is a complete assay system designed to measure histone deacetylase 11 (HDAC11) activity for screening and profiling applications. The HDAC11 Fluorogenic Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC11 activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC11. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader._x000D_HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. The amount of fluorescence is therefore directly related to the level of HDAC activity._x000D_ |
Fluorogenic Sirtuin 6 Substrate |
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50866 | BPS Bioscience | 50 µl | EUR 235 |
Description: A fluorogenic, myristoylated lysine peptide substrate for Sirtuin 6. This substrate should be used in conjunction with SIRT Developer, #50089. Optimal excitation wavelength 350-380 nm, emission wavelength 440-460 nm. |
IDO1 Fluorogenic Reaction Solution |
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73009 | BPS Bioscience | 10 ml | EUR 205 |
Description: IDO1 Fluorogenic Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO1-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS Human IDO1 Fluorogenic Inhibitor Screening Assay Kit (BPS Cat. #72037 &Cat. #72047) and/or with purified, active IDO1, His-tag (BPS Cat. #71182) in combination with 1x IDO1 Assay Buffer (BPS Cat. #73002). |
TDO Fluorogenic Reaction Solution |
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73010 | BPS Bioscience | 10 ml | EUR 205 |
Description: TDO Fluorogenic Reaction Solution is an optimized, proprietary_x000D_formulation containing all the necessary reaction components for TDO-catalyzed_x000D_conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays._x000D_This product is intended to be used with the BPS Human TDO Fluorogenic Inhibitor_x000D_Screening Assay Kits (BPS Cat. #72039 & Cat. #72049) and/or with purified, active TDO,_x000D_His-tag (BPS Cat. #71195) in combination with 1x TDO Assay Buffer (BPS Cat. #73006). |