Zinc (Zn, atomic number: 30) is an essential mineral of great biological importance. It is a metal with characteristics similar to Magnesium due to its size and oxidation state +2. It is the second
Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR)
The methodologies of the directed evolution benefit from quantitative reading quantitatively genotype at the phenotype. We have therefore designed a method flowing from protein-peptide interactions
Transient Stem-Loop Structure of Nucleic Acid Template May Interfere with Polymerase Chain Reaction through Endonuclease Activity of Taq DNA Polymerase
As a standard molecular biology technique, PCR uses DNA polymerase to detect, amplify and manipulate DNA targets. Due to its exponential amplification effect, the PCR can achieve a high sensitivity
Development of three duplex real-time RT-PCR assays for the sensitive and rapid detection of a phytoplasma and five viral pathogens affecting stone fruit trees
Trial chain tests of the three duplex transcription of the three duplex transcription (real-time RT-PCR) transcription based on Taqman chemistry, have been developed for simultaneous detection and
DNA analysis is a key procedure in genetic engineering. Nowadays, the analysis is often made by PCR with the Taq DNA polymerase. Although the last enzyme price is quite low, the demand for many
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.
The polymerases are enzymes that synthesize long chains or nucleic acid polymers, including DNA or Nucleotide RNA. They assemble nucleic acids by copying a strand of DNA or RNA model using basic
Microsatellite (SSR) amplification by PCR usually led to polymorphic bands: Evidence which shows replication slippage occurs in extend or nascent DNA strands.
Microsatellites or simple sequence repetitions (SSR) are very effective molecular markers in the population genetics, genome mapping, taxonomic study and other large-scale studies. The variation in
Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering.
DNA polymerase is widely used for manipulating DNA in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. thermostable DNA polymerase is very useful and can be
Reduced reaction volumes and increased Taq DNA polymerase concentration improve STR profiling outcomes from a real-world low template DNA source: telogen hairs.
OBJECTIVEThe main method for the analysis of low template DNA (LTDNA) is known as a method of low copy number (LCN), which involves increasing the number of PCR cycles (typically 34). In common with
Effect of surface hydrophobicity on activity of immobilized Taq DNA polymerase, its reusability and storage.
Oriented methods and activity-preserved immobilization of biologically active proteins based on the concept of active-site masking and previously reported kinetic control. We extended our research