Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.

Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.

BACKGROUND: Detection of bacteria by PCR applied for screening blood and blood products with special attention platelet concentrates. For practical use is expected that the detection system including Gram-positive, Gram-negative and Gram-bacterial non-stainable. It is quite challenging to achieve high sensitivity with clear negative controls with PCR reagents, due mainly Taq DNA polymerase is contaminated with traces of bacteria.

METHODS: Bacterial decontamination of Taq DNA polymerase tested by two different methods using restriction enzymes Sau 3A1 and microfiltration. Besides the commercially available Taq polymerase depleted bacterial DNA was included.A published real-time PCR specific for Gram-negative bacteria adapted to Gram-positive bacteria, including Staphylococcus species specific and Mycobacteria, and is used to fill three Taq polymer-ases discharged from bacteria

RESULTS DNA contamination: Despite published reports of successful DNA decontamination , all three approaches perform poorly in experiments conducted in this study. Sensitivity ranges from about 50-100 colony units (CFU) per PCR reaction to Escherichia coli and Staphylococcus epidermidis, corresponding to 1.250 to 2.500 CFU / ml sample material. Conclusion: It seems unsatisfactory to receive a high detection limit for the PCR bacteria even if highly multiplexed diagnostics. a reliable method for the decontamination of Taq DNA polymerase is required and will present one important step towards the detection of bacterial DNA with high sensitivity.


DNA analysis is the main procedure in genetic engineering. This time the analysis is often done by PCR with Taq DNA polymerase. Although the price of the last enzyme is quite low, the demand for analysis of various results in a lot of spending money that is not affordable for many laboratories. Meanwhile, many screening duties do not require highly purified enzyme. Taking into account the unique nature of the enzyme that allows for little simplifies the production without the use of expensive techniques such as column chromatography or the length and / or dialysis.

The following data routine use of Taq DNA polymerase is prepared in accordance with the protocol developed in our laboratory are presented. protocols only take a few hours to realize and does not need to be a qualified technician or costly equipment. But give an enzyme preparation suitable for most screening purposes. Taq DNA polymerase isolated stock can be stored as ammonium sulfate suspension in the refrigerator for a long time, not less than 6 months. Working enzyme solution prepared from stock suspension on demand, no more than once a month and can be stored well in the refrigerator.

 Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.
Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.

Selection of Taq DNA polymerase efficiently to optimize the T-DNA genotyping method for the rapid detection of mutant Arabidopsis thaliana plants.

Port plant homolog of various animal genes involved in the metabolism of phosphorus, telomere biology and other cellular processes. Compared to experiment with many other multicellular organisms, research in the model plant Arabidopsis thaliana take advantage of a short generation time and increasing warehouse of genetic tools and GMOs, including a large collection of T-DNA knockout and line activation.

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 108

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 746.87

Taq DNA Polymerase

BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

L7051001 1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

9001-2500
EUR 512

Taq DNA Polymerase

9001-500
EUR 191

Tsg DNA Polymerase, 5u/ul

D0081 5X200U
EUR 93.5

T4 DNA Polymerase, 5u/ul

BEP0061 100U
EUR 128.31

Pfu DNA Polymerase, 5u/ul

B0093(D0090P) 500U
EUR 137

Maximo Taq DNA Polymerase

S101 500 units
EUR 46

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 107

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 328

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 153
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 257
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 140
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 218
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 174.41

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 485

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1028.75

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 348.85

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 592

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 230

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 328

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 131

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 192

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 313

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 205

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 87

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 309

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 568
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 177

Taq Plus DNA Polymerase

P201-01 250 U
EUR 123

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 178

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 319

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S111 500 units
EUR 53

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S112 5x500 units
EUR 124

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S128 20x500 units
EUR 406

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)

S129 100x500 units or as bulk
EUR 1785

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U
EUR 217

ACTaq? Taq DNA Polymerase, 250U

E2100-250U
EUR 102

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 350.54

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 592

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 230

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 328

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 517

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 333

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 239

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 164

Taq Plus DNA Polymerase (PCR)

G012 250 U (50 ul)
EUR 73

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
EUR 106

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

Taq DNA Polymerase Polyclonal Antibody

7018-100
EUR 354

Taq DNA Polymerase Polyclonal Antibody

7018-30T
EUR 146

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Bst DNA Polymerase, Large Fragment

M1213-200
EUR 289

Bsu DNA Polymerase Large Fragment

M1214-200
EUR 398

Bst DNA Polymerase Large Fragment

P701-01 800 U
EUR 122

Bst DNA Polymerase Large Fragment

P701-02 8000 U
EUR 271

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U
EUR 227

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U
EUR 105

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 147

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 228

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 296
Description: Minimum order quantity: 1 unit of 500EU

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1157

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 5450

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 131

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-100 2x500 units
EUR 192

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-200 4x500 units
EUR 313

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 164

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 397

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 262

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 193

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 262

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 120

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 367

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

DNA Polymerase I Large (Klenow) Fragment

M1212-100
EUR 278

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-010 100 units
EUR 150

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3

P0330-020 2x100 units
EUR 239

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-050 5X100 units
EUR 388

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-250 25X100 units
EUR 1670

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-500 50X100 units
EUR 2993

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1280.62

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 292.73

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 907.39

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 432.37

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 190

Hot Start Taq DNA Polymerase - 1000 units

3293 1/EA
EUR 310

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

Tsg PlusDNA Polymerase, 5u/ul

D0088 200U
EUR 79

Accuris Taq Polymerase

PR1000-1000 1 PC
EUR 172.66

Accuris Taq Polymerase

PR1000-500 1 PC
EUR 132.79

Accuris Taq Polymerase

PR1000-6000 1 PC
EUR 618.47

Accuris Taq Polymerase

PR1000-S 1 PC
EUR 69.79

Bsu DNA Polymerase Large Fragment - 1,000 units

3585 1/EA
EUR 305

Sau DNA Polymerase Large Fragment - 1,000 units

3595 1/EA
EUR 508

Reversible 1-min Hot Start Taq DNA Polymerase

P950 - Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase

P950L - Ask for price

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 165

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 300

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 583

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-100 100,000 units
EUR 2885

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B01 10,000 units
EUR 1157

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

T2201-B05 5x10,000 units
EUR 5450

Genorise® CLT Taq DNA Polymerase (1000 U)

GR108003 1000 U
EUR 199

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 409

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 136

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 267

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 409

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1525

T4 DNA Ligase, 5u/ul

B1122 1KU
EUR 158.75

T4 DNA Ligase, 5u/ul

B1125 200U
EUR 77.84

EZ Taq Polymerase (HotStart)

9K-005-0020 500U
EUR 204.86

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-025 250 unit
EUR 167

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-050 2X250 unit
EUR 250

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-100 4X250 Unit
EUR 421

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-250 10X250 Unit
EUR 739

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 479.35

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2054.48

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 320.14

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 879.98

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2399

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 669

Cenarchaeum symbiosum DNA polymerase II large subunit (polC)

1-CSB-EP370095DRA
  • EUR 621.00
  • EUR 381.00
  • EUR 1943.00
  • EUR 882.00
  • EUR 1335.00
  • EUR 451.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial expressed in E.coli

DNA Polymerase I, Large (Klenow) Fragment - 1,000 units

3262 1/EA
EUR 289

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S113 2x100 rcs
EUR 66

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)

S114 10x100 rcs
EUR 193

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 72

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 193

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-050 500 units
EUR 165

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-102 25x500 units
EUR 1427

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-500 10X500 units
EUR 739

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

Fast DNA Ligation Kit (5u/ul, T4)

BS511 10prep
EUR 70.88

Fast DNA Ligation Kit (5u/ul, T4)

BS512 50prep
EUR 132.65

T4 DNA Ligase with PEG, 5u/ul

B1442 1KU
EUR 163.1

T4 DNA Ligase with PEG, 5u/ul

B1445 200U
EUR 80.45

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-500 500 units
EUR 219

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-502 2x500 units
EUR 354

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-510 10x500 units
EUR 1373

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-525 25x500 units
EUR 3020

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-550 50x500 units
EUR 5383

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 357

Taq DNA Ligase - 10,000 units

3219 1/EA
EUR 253

Recombinant MeV Large Polymerase Protein

VAng-Lsx0385-inquire inquire Ask for price
Description: Recombinant MeV Large Polymerase containing the large polymerase immunodominant regions, 58-149 amino acids was expressed in E. coli and purified by proprietary chromatographic technique.

Pfu DNA Polymerase

S116 1x250 units
EUR 76

Pfu DNA Polymerase

S117 2x250 units
EUR 94

Pfu DNA Polymerase

S118 10x250 units
EUR 303

Bst DNA Polymerase

S600 2000 U
EUR 107

DNA polymerase ? (Rat)

10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

Available to the public availability of thousands of transgenic Arabidopsis lines provide a unique opportunity to address a number of important biological questions. However, the identification of T-DNA mutant of an individual plant seedlings provided by the distribution centers stock biological remains laborious and time-consuming procedure.

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